Visual detection of <i>Cronobacter sakazakii</i> on a microfluidic chip fabricated by a 3D molding method

Chen, Xu; Ma, Yue; Miao, Shuangyu; Li, Dongnan; Zhang, Ye

doi:10.1039/d2an02002e

Cited by 4 publications

(1 citation statement)

References 34 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…53,54 Indeed AM can bring about outcomes comparable to traditional lithography in terms of temporal and spatial resolution, material, and mechanical properties while being cost-effective, rapid, and scalable. 55,56 While the integration of LAMP onto additively manufactured devices is gaining traction, 20,[57][58][59] some challenges still exist with the combination of all the sequential steps onto a modular userfriendly platform.…”

Section: Introductionmentioning

confidence: 99%

Additive manufacturing leveraged microfluidic setup for sample to answer colorimetric detection of pathogens

Yedire,

Hosseini,

Shieh

et al. 2023

Lab Chip

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Additive manufacturing leveraged microfluidic setup for sample to answer colorimetric detection of pathogens

Yedire,

Hosseini,

Shieh

et al. 2023

Lab Chip

View full text Add to dashboard Cite

show abstract

Novel insights into the integrated and extensive approach for microbiological quality and safety of powdered infant formula

Cho,

Rhee

2023

Current Opinion in Food Science

View full text Add to dashboard Cite

Additive Manufacturing Leveraged Microfluidic Setup for Sample to Answer Colorimetric Detection of Pathogens

Yedire,

Hosseini,

Shieh

et al. 2023

Preprint

View full text Add to dashboard Cite

Colorimetric readout for the detection of infectious diseases is gaining traction at the point of care/need owing to its ease of analysis and interpretation, and integration potential with highly specific Loop-mediated amplification (LAMP) assays. However, coupling colorimetric readout with LAMP is rife with challenges including, rapidity, inter-user variability, colorimetric signal quantification, and user involvement in sequential steps of the LAMP assay, hindering its application. To address these challenges, for the first time, we propose a remotely smartphone-operated automated setup consisting of (i) an additively manufactured microfluidic cartridge, (ii) a portable reflected-light imaging setup with controlled epi-illumination (PRICE) module, and (iii) a control and data analysis module. The microfluidic cartridge facilitates sample collection, lysis, mixing of amplification reagents stored on-chip, and subsequent isothermal heating for initiation of amplification in a novel way by employing tunable elastomeric chambers and auxiliary components (heaters and linear actuators). PRICE offers a new imaging setup that captures the colorimetric change of the amplification media over a plasmonic nanostructured substrate in a controlled and noise-free environment for rapid minute-scale nucleic acid detection. The control and data analysis module employs microprocessors to automate cartridge operation in tandem with the imaging module. The different device components were characterized individually and finally, as a proof of concept, SARS-CoV-2 wild-type RNA was detected with a turnaround time of 13 minutes, showing the clinical feasibility. The suggested automated device can be adopted in future iterations for other detection and molecular assays that require sequential fluid handling steps.

show abstract

Visual detection of Cronobacter sakazakii on a microfluidic chip fabricated by a 3D molding method

Abstract: PDMS microfluidic chips were fabricated using a 3D molding method and a multiplex CAMP assay was proposed on the chip for the visual detection of C. sakazakii in powdered infant formula.

Cited by 4 publications

References 34 publications

Additive manufacturing leveraged microfluidic setup for sample to answer colorimetric detection of pathogens

Additive manufacturing leveraged microfluidic setup for sample to answer colorimetric detection of pathogens

Novel insights into the integrated and extensive approach for microbiological quality and safety of powdered infant formula

Additive Manufacturing Leveraged Microfluidic Setup for Sample to Answer Colorimetric Detection of Pathogens

Contact Info

Product

Resources

About