Visual detection of odorant geraniol enabled by integration of a human olfactory receptor into polydiacetylene/lipid nano-assembly

Kim, Tae-Gon; Moon, Dongseok; Park, Jin Hyuk; Yang, Heehong; Cho, Seongyeon; Park, Tai Hyun; Ahn, Dong June

doi:10.1039/c9nr00249a

Cited by 10 publications

(10 citation statements)

References 41 publications

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“…The chromogenic nature of PDAs endows a fascinating property of stimuli responsiveness with the typical blue-to-red colorimetric transition and has been utilized extensively for fabricating various sensing devices. − Since the blue-phase polymeric material of PMCDA-H + can be easily prepared by UV irradiation, the colorimetric response of PMCPDA-H + was tested against heat, organic solvents, and base (alkaline pH) for exploring the possibility of devising colorimetric sensor systems. For this purpose, the blue-phase PMCPDA-H + coated sensors were fabricated on a glass slide by a simple drop-casting technique followed by UV-light irradiation.…”

Section: Resultsmentioning

confidence: 99%

Protonation-Induced Self-Assembly of Flexible Macrocyclic Diacetylene for Constructing Stimuli-Responsive Polydiacetylene

2019

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The shape-persistent self-assembling characteristic of the macrocyclic structure has been extensively employed for creating columnar nanoarchitectures. The macrocyclic structure offers a definite structural confinement as well as conformational flexibility to the molecular frame and ensures the unidirectional self-assembly into hollow tubular channels. Upon introducing the judicious choice of functional groups, the macrocycle often functions as an adaptive receptor/host and accommodates structure-specific external guest within the predefined confined space. A new macrocycle, PMCDA, was constructed from a more flexible polymerizable diacetylene (DA) template by connecting with pyridine rings. Owing to the proton receptor nature of pyridine ring and π–π stacking characteristic of DA template, protonation-induced tubular structures are generated through the columnar assembly of PMCDA. Upon UV light irradiation, the monomeric PMCDA-H + are transformed into the robust covalently cross-linked blue-phase polydiacetylene (PMCPDA-H + ). Quite interestingly, the blue-phase PMCPDA-H + displays multistimuli-responsive colorimetric sensory responses: reversible thermochromism, selective solvatochrom for dimethyl sulfoxide and dimethylformamide, and organic/inorganic base sensing. The chromatic changes of PMCPDA-H + demonstrate potential applications in developing thermo-chemocolorimetric sensors.

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Section: Resultsmentioning

confidence: 99%

Protonation-Induced Self-Assembly of Flexible Macrocyclic Diacetylene for Constructing Stimuli-Responsive Polydiacetylene

2019

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“…Monochrome fluorescence images of the particles were taken on an inverted fluorescence microscope (Axiovert 200, Zeiss, Germany) connected with a scientific complementary metal-oxidesemiconductor (CMOS) camera (Prime, Photometrics, USA) and a fluorescence light source (Illuminator HXP 120V, Zeiss, Germany). For detecting fluorescence signals from Alexa fluor 488 cadaverine, biotin-PEG-FITC, and Alexa fluor 488 conjugated 2nd antibody, the light was filtered using a green fluorescence filter set (λex/λem = 492/517 nm) [44,45]. The images were taken using 20× objective with a camera exposure time of 100 ms.…”

Section: Image Analysismentioning

confidence: 99%

Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography

Lee

Maeng

Kim

et al. 2020

JCM

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Technologies for the detection and isolation of circulating tumor cells (CTCs) are essential in liquid biopsy, a minimally invasive technique for early diagnosis and medical intervention in cancer patients. A promising method for CTC capture, using an affinity-based approach, is the use of functionalized hydrogel microparticles (MP), which have the advantages of water-like reactivity, biologically compatible materials, and synergy with various analysis platforms. In this paper, we demonstrate the feasibility of CTC capture by hydrogel particles synthesized using a novel method called degassed mold lithography (DML). This technique increases the porosity and functionality of the MPs for effective conjugation with antibodies. Qualitative fluorescence analysis demonstrates that DML produces superior uniformity, integrity, and functionality of the MPs, as compared to conventional stop flow lithography (SFL). Analysis of the fluorescence intensity from porosity-controlled MPs by each reaction step of antibody conjugation elucidates that more antibodies are loaded when the particles are more porous. The feasibility of selective cell capture is demonstrated using breast cancer cell lines. In conclusion, using DML for the synthesis of porous MPs offers a powerful method for improving the cell affinity of the antibody-conjugated MPs.

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“…After the calibration of the colorimetric response against the concentration of the ligand in solution, PDA can be used as a colorimetric binding assay. 18,19 In contrast, the Jelinek group has discovered that different kinds of antimicrobial peptides stimulate PDAs embedded in phospholipid bilayers at various levels in the absence of such a specific interaction. 20,21 This indicates that the PDA color reflects the nature of the lipid−peptide interactions, opening a potential use of PDA as a functional assay.…”

Section: ■ Introductionmentioning

confidence: 99%

“…For the recognition of a specific molecule, often the head group of the PDA is functionalized so that it specifically binds to the ligand of interest. After the calibration of the colorimetric response against the concentration of the ligand in solution, PDA can be used as a colorimetric binding assay. , …”

Section: Introductionmentioning

confidence: 99%

Analysis of PDA Dose Curves for the Extraction of Antimicrobial Peptide Properties

Zhao

Sugihara

2021

J. Phys. Chem. B

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A mechanochromic polymer, polydiacetylene, changes color upon ligand binding, being a popular material in biosensing. However, whether it can also detect ligand functions in addition to binding is left understudied. In this work, we report that the polydiacetylene can be used to determine the net charges and the mode of actions (carpet model, toroidal pore model, etc.) of antimicrobial peptides and detergents via EC50 and Hill coefficients from the colorimetric response–dose curves. This opens a potential for high-throughput peptide screening by functions, which is difficult with the conventional methods.

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Visual detection of odorant geraniol enabled by integration of a human olfactory receptor into polydiacetylene/lipid nano-assembly

Abstract: We fabricated a polydiacetylene lipid/human olfactory receptor nano-assembly for the “visualization of odorant detection” upon specific interaction with the target odorant, triggering a colorimetric transition and a distinctive fluorescence increase.

Cited by 10 publications

References 41 publications

Protonation-Induced Self-Assembly of Flexible Macrocyclic Diacetylene for Constructing Stimuli-Responsive Polydiacetylene

Protonation-Induced Self-Assembly of Flexible Macrocyclic Diacetylene for Constructing Stimuli-Responsive Polydiacetylene

Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography

Analysis of PDA Dose Curves for the Extraction of Antimicrobial Peptide Properties

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