Visual detection of peptidase activity using fluorogenic substrates in a microtiter plate assay

Irvine, G. Brent; Ennis, M.P.; Williams, C. H.

doi:10.1016/0003-2697(90)90298-n

Cited by 19 publications

(7 citation statements)

References 6 publications

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“…The enzyme assays were carried out using Suc-Ala-Ala-Pro-Phe-AMC oligopeptide as a substrate. [32][33][34][35] In this system AMC becomes uorescent, when it is freed aer the peptide bond is hydrolysed. According to the activity assays, the protease encapsulated in the silica nanocapsules exhibits at 25 C in 0.1 M Tris-HCl buffer of pH 8.5 a catalytic activity of 55.27 AE 7.15 U mg À1 min À1 , meanwhile the activity of the free protease under the same conditions is 142.82 AE 5.82 U mg À1 min À1 , and it remains unchanged aer stirring in the same medium as that used for the encapsulation experiments (i.e.…”

Section: Resultsmentioning

confidence: 99%

Encapsulation of enzymes in silica nanocapsules formed by an amphiphilic precursor polymer in water

Zhang

Yan

et al. 2015

J. Mater. Chem. B

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Section: Resultsmentioning

confidence: 99%

Encapsulation of enzymes in silica nanocapsules formed by an amphiphilic precursor polymer in water

Zhang

Yan

et al. 2015

J. Mater. Chem. B

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“…The application and sensitivity of coumarin derivatives in measuring peptidase activity has been widely reported (15,(35)(36)(37). Gallagher et al (16,17) recently described fluorometric discontinuous assays in which TRHMCA was employed as the substrate for TRH-DE.…”

Section: Discussionmentioning

confidence: 99%

Development of a Continuous, Fluorometric Coupled Enzyme Assay for Thyrotropin-Releasing Hormone-Degrading Ectoenzyme

Kelly

Slator

Tipton

et al. 1999

Analytical Biochemistry

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“…Based on this property, Zimmerman et al first synthesized glutaryl-Phe-NH-Mec as a substrate for chymotrypsin [46]. Subsequently, many other AMC-based probes have been developed to detect enzymatic activity in cells, homogenates and solutions [47][48][49][50].…”

Section: Spectroscopic Probes With Peptidase-or Proteasecleavable Pepmentioning

confidence: 99%

Progress in Spectroscopic Probes with Cleavable Active Bonds

Chen¹,

Sun²,

2006

COC

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Spectroscopic probes may be defined as the molecules that can react with analytes (targets) accompanying the changes of their spectroscopic (chromogenic, fluorescent, or chemiluminescent) properties; based on such changes the targets can thus be determined. Spectroscopic probes have been extensively investigated and used widely in many fields because of their powerful ability to improve analytical sensitivity and to offer greater temporal and spatial sampling capability. In this review, special interest is devoted to a new type of spectroscopic probe that is constructed with a cleavable active bond as a linker. This type of spectroscopic probe, developed greatly in the past few years, has opened a novel alternate route to the specific determination of analytes with high hydrolytic reactivity, e.g., from metal ions to enzyme activity, and enabled many biological processes to be monitored in situ and in real-time. Theoretically, various photophysical processes, such as photoinduced electron transfer, photoinduced proton transfer, and fluorescence resonance energy transfer, can be used to design spectroscopic probes with cleavable active bonds for selective detection of analytes. Herein we review the progress and application of this type of spectroscopic probe, including spectroscopic response mechanism and the probes with active bonds cleavable by enzyme, metal ion and reactive oxygen species.

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Visual detection of peptidase activity using fluorogenic substrates in a microtiter plate assay

Cited by 19 publications

References 6 publications

Encapsulation of enzymes in silica nanocapsules formed by an amphiphilic precursor polymer in water

Encapsulation of enzymes in silica nanocapsules formed by an amphiphilic precursor polymer in water

Development of a Continuous, Fluorometric Coupled Enzyme Assay for Thyrotropin-Releasing Hormone-Degrading Ectoenzyme

Progress in Spectroscopic Probes with Cleavable Active Bonds

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