Visual electrochemiluminescence detection of telomerase activity based on multifunctional Au nanoparticles modified with G-quadruplex deoxyribozyme and luminol

Zhang, Huairong; Wang, Yin-Zhu; Wu, Mei-Sheng; Feng, Qiumei; Shi, Hang; Chen, Hong‐Yuan; Xu, Jing‐Juan

doi:10.1039/c4cc06302c

Cited by 75 publications

(35 citation statements)

References 20 publications

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“…A readily detectable PGM signal was distinguishable from the background signal (S/N = 3) in the presence of telomerase-containing extracts of 20 HeLa cells. This sensitivity is comparable to those of most elaborate instrument-based methods 25 34 46 . Simultaneously, the proposed method also exhibits a significantly enhanced dynamic range compared to instrument-based methods.…”

Section: Resultsmentioning

confidence: 59%

“…Additionally, this method is not applicable to the detection of short telomerase elongation products, such as telomerase substrate primers with two repetitive sequences of (TTAGGG) 17 . Alternative methods seeking to resolve these challenges include in situ monitoring methods that use gold nanoparticles and mesoporous silica nanoparticles 18 19 , colorimetric 20 21 22 , fluorescent 23 24 25 26 27 28 , electrochemistry-based methods 29 30 31 32 , electrochemiluminescence-based methods 33 34 , surface plasmon resonance (SPR) methods 35 36 , and enzyme-linked immunosorbent (ELISA) assays 37 . These methods are useful platforms for telomerase activity detection and related research, but the use of relatively complex instruments limits their further application in remote and less-developed areas where medical facilities and resources are limited.…”

mentioning

confidence: 99%

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Coupling a DNA-Based Machine with Glucometer Readouts for Amplified Detection of Telomerase Activity in Cancer Cells

Wang

Huang

et al. 2016

Sci Rep

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The strong correlation between cancer and telomerase activity has inspired the development of new strategies to evaluate telomerase activity. Here, a personal glucose meter (PGM) system that uses DNA-based machine amplification to detect telomerase in cancer cells is reported. In this assay, telomerase elongation products are amplified in the form of another type of product by a DNA-based machine. This process can only be activated by the hybridization of the extended telomerase substrate (TS) probe and the complementary primer in the presence of telomerase. The obtained products are then transformed to glucose-related signals via a three-component assay, which enables the simple use of a PGM to indirectly quantify the telomerase activity. The proposed method realizes sensitive telomerase activity detection down to 20 HeLa cells with a significantly enhanced dynamic range. Additionally, short telomerase elongation products, such as telomerase substrate probes with two repetitive sequences, that cannot be detected using the most widely used telomeric repeat amplification protocol assay were detected.

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Section: Resultsmentioning

confidence: 59%

mentioning

confidence: 99%

Coupling a DNA-Based Machine with Glucometer Readouts for Amplified Detection of Telomerase Activity in Cancer Cells

Wang

Huang

et al. 2016

Sci Rep

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“…Hitherto, a variety of methods have been developed to investigate the telomerase activity by using various techniques, such as fluorescence 12 – 15 , electrochemistry 16 – 19 , surface enhanced Raman scattering 20 , surface plasma resonance 21 , 22 , colorimetry 23 – 25 , chemiluminescence 26 , and electrochemiluminescence 27 , 28 . However, these technologies can only analyze the bulk samples in vitro , such as cell extraction and urine 12 , 14 .…”

Section: Introductionmentioning

confidence: 99%

Molecular imaging of telomerase and the enzyme activity-triggered drug release by using a conformation-switchable nanoprobe in cancerous cells

Shi

Gao

Liu

et al. 2018

Sci Rep

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So far, the development of a unique strategy for specific biomolecules activity monitoring and precise drugs release in cancerous cells is still challenging. Here, we designed a conformation-switchable smart nanoprobe to monitor telomerase activity and to enable activity-triggered drug release in cancerous cells. The straightforward nanoprobe contained a gold nanoparticle (AuNP) core and a dense layer of 5-carboxyfluorescein (FAM)-labeled hairpin DNA shell. The 3′ region of hairpin DNA sequence could function as the telomerase primer to be elongated in the presence of telomerase, resulting in the conformational switch of hairpin DNA. As a result, the FAM fluorescence was activated and the anticancer drug doxorubicin (Dox) molecules which intercalated into the stem region of the hairpin DNA sequence were released into cancerous cells simultaneously. The smart method could specifically distinguish cancerous cells from normal cells based on telomerase activity. It also showed a good performance for monitoring telomerase activity in the cytoplasm by molecular imaging and precise release of Dox triggered by telomerase activity in cancerous cells. These advantages may offer a great potential of this method for monitoring telomerase activity in cancer progression and estimating therapeutic effect.

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“…DNA with G‐rich or C‐rich sequences can form G‐quadruplex and i‐motif structures. Therefore, AuNPs conjugated to these DNA have been utilized for the detection of protein kinases and telomerases (a specialized reverse transcriptase) . In addition to sensing application, DNA–AuNPs have been demonstrated as potential drugs and drug carriers .…”

Section: Introductionmentioning

confidence: 99%

Biomedical Applications of DNA‐Conjugated Gold Nanoparticles

Wang

et al. 2016

ChemBioChem

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Gold nanoparticles (AuNPs) are useful for diagnostic and biomedical applications, mainly because of their ease in preparation and conjugation, biocompatibility, and size-dependent optical properties. However, bare AuNPs do not possess specificity for targets. AuNPs conjugated with DNA aptamers offer specificity for various analytes, such as proteins and small molecules/ions. Although DNA aptamers themselves have therapeutic and target-recognizing properties, they are susceptible to degradation in vivo. When DNA aptamers are conjugated to AuNPs, their stability and cell uptake efficiency both increase, making aptamer-AuNPs suitable for biomedical applications. Additionally, drugs can be efficiently conjugated with DNA aptamer-AuNPs to further enhance their therapeutic efficiency. This review focuses on the applications of DNA aptamer-based AuNPs in several biomedical areas, including anticoagulation, anticancer, antibacterial, and antiviral applications.

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Visual electrochemiluminescence detection of telomerase activity based on multifunctional Au nanoparticles modified with G-quadruplex deoxyribozyme and luminol

Abstract: A novel visual electrochemiluminescence (ECL) analysis strategy for detection of telomerase activity is reported on a microarray chip, with G-quadruplex deoxyribozyme (DNAzyme) and luminol modified Au nanoparticles (NPs) as double-catalytic amplification labels.

Cited by 75 publications

References 20 publications

Coupling a DNA-Based Machine with Glucometer Readouts for Amplified Detection of Telomerase Activity in Cancer Cells

Coupling a DNA-Based Machine with Glucometer Readouts for Amplified Detection of Telomerase Activity in Cancer Cells

Molecular imaging of telomerase and the enzyme activity-triggered drug release by using a conformation-switchable nanoprobe in cancerous cells

Biomedical Applications of DNA‐Conjugated Gold Nanoparticles

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