2023
DOI: 10.1101/2023.10.19.563166
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Visual impairment cell non-autonomously dysregulates brain-wide proteostasis

Shashank Shekhar,
Katherine J Wert,
Helmut Krämer

Abstract: Loss of hearing or vision has been identified as a significant risk factor for dementia but underlying molecular mechanisms are unknown. In different Drosophila models of blindness, we observe non-autonomous induction of stress granules in the brain and their reversal upon restoration of vision. Stress granules include cytosolic condensates of p62, ATF4 and XRP1. This cytosolic restraint of the ATF4 and XRP1 transcription factors dampens expression of their downstream targets during cellular stress. Cytosolic … Show more

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“…Primary antibodies were incubated overnight at 4°C. The following antibodies were used: rabbit anti-ATG8 (1:1000, abcam# ab109364), rabbit anti-p62 (1:5000, generously provided by Helmut Kramer 65 ), anti-rabbit RFP (1:1000, Rockland 600 401 379), mouse anti-tubulin (1:4000, Developmental Studies Hybridoma Bank #E7c), and mouse anti-V5 (1:5000,ThermoFisher Scientific). All HRP-conjugated secondary antibodies (Jackson Immunochemicals) were used at 1:10000 dilutions and incubated for 2 hours at room temperature.…”
Section: Methods Detailsmentioning
confidence: 99%
“…Primary antibodies were incubated overnight at 4°C. The following antibodies were used: rabbit anti-ATG8 (1:1000, abcam# ab109364), rabbit anti-p62 (1:5000, generously provided by Helmut Kramer 65 ), anti-rabbit RFP (1:1000, Rockland 600 401 379), mouse anti-tubulin (1:4000, Developmental Studies Hybridoma Bank #E7c), and mouse anti-V5 (1:5000,ThermoFisher Scientific). All HRP-conjugated secondary antibodies (Jackson Immunochemicals) were used at 1:10000 dilutions and incubated for 2 hours at room temperature.…”
Section: Methods Detailsmentioning
confidence: 99%