“…Primary antibodies were incubated overnight at 4°C. The following antibodies were used: rabbit anti-ATG8 (1:1000, abcam# ab109364), rabbit anti-p62 (1:5000, generously provided by Helmut Kramer 65 ), anti-rabbit RFP (1:1000, Rockland 600 401 379), mouse anti-tubulin (1:4000, Developmental Studies Hybridoma Bank #E7c), and mouse anti-V5 (1:5000,ThermoFisher Scientific). All HRP-conjugated secondary antibodies (Jackson Immunochemicals) were used at 1:10000 dilutions and incubated for 2 hours at room temperature.…”