Visual LAMP Method for Detection of Vibrio vulnificus in Aquatic Products and Environmental Water

Abstract

Background A visualized, rapid, simple method was developed based on loop-mediated isothermal amplification (LAMP) assay to detect Vibrio vulnificus in aquatic products and aquaculture waters.Results Genomic DNA was extracted from Vibrio vulnificus using the boiling and column extraction methods and optimized primers were used to detect the gyrB gene using a visual LAMP method. The sensitivity of the assay was 10 fg/μL, and the results were stable and reliable. Of 655 aquatic product samples and 558 aquaculture waters samples, the positive rates of Vibrio vulnificus detection were 9.01% and 8.60%, respectively, which is markedly higher than that of the traditional culture identification methods. Conclusion The relatively simple technical requirements, low equipment costs, and rapid detection time make the visual LAMP method for detection of Vibrio vulnificus a convenient choice for field diagnosis in the aquaculture industry.