Visual object detection biases escape trajectories following acoustic startle in larval zebrafish

Zwaka, Hanna; McGinnis, Olivia J; Pflitsch, Paula; Prabha, Srishti; Mansinghka, Vikash K.; Engert, Florian; Bolton, Andrew D.

doi:10.1016/j.cub.2022.10.050

Cited by 11 publications

(3 citation statements)

References 47 publications

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“…The escape response in zebrafish is a rapid evasion behavior typically triggered in response to external sound stimuli or dangerous objects such as natural enemies. And the key neurons that drive the escape response are Mauthner cells [ 48 , 49 ]. Mauthner neuron cell bodies located in the brain can sense stimuli and transmit signals to the spinal cord, thereby driving muscles to move away from the stimulus [ 50 , 51 ].…”

Section: Resultsmentioning

confidence: 99%

“…Certainly, the escape response in zebrafish encompasses two distinct behaviors: C-start, which is propelled by the Mauthner cells, and the escape swimming behavior. Previous studies have established a notable correlation between the recuperation of Mauthner cells and the C-start response [ 48 – 51 ]. Our above results revealed that her6 −/− zebrafish had a significant increase in axon regeneration (Fig.…”

Section: Resultsmentioning

confidence: 99%

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MicroRNA-9 promotes axon regeneration of mauthner-cell in zebrafish via her6/ calcium activity pathway

Shen,

Chen,

Song

et al. 2024

Cell. Mol. Life Sci.

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MicroRNA (miRNA), functioning as a post-transcriptional regulatory element, plays a significant role in numerous regulatory mechanisms and serves as a crucial intrinsic factor influencing axon regeneration. Prior investigations have elucidated the involvement of miRNA-9 in various processes, however, its specific contribution to axon regeneration in the central nervous system (CNS) remains uncertain. Hence, the zebrafish Mauthner axon regeneration model was employed to manipulate the expression of miRNA-9 in single cells, revealing that upregulation of miRNA-9 facilitated axon regeneration. Additionally, her6, a downstream target gene of miRNA-9, was identified as a novel gene associated with axon regeneration. Suppression of her6 resulted in enhanced Mauthner axon regeneration, as evidenced by the significantly improved regenerative capacity observed in her6 knockout zebrafish. In addition, modulation of her6 expression affects intracellular calcium levels in neurons and promoting her6 expression leads to a decrease in calcium levels in vivo using the new NEMOf calcium indicator. Moreover, the administration of the neural activity activator, pentylenetetrazol (PTZ) partially compensated for the inhibitory effect of her6 overexpression on the calcium level and promoted axon regeneration. Taken together, our study revealed a role for miRNA-9 in the process of axon regeneration in the CNS, which improved intracellular calcium activity and promoted axon regeneration by inhibiting the expression of downstream target gene her6. In our study, miRNA-9 emerged as a novel and intriguing target in the intricate regulation of axon regeneration and offered compelling evidence for the intricate relationship between calcium activity and the facilitation of axon regeneration. Graphical Abstract miRNA-9 can promote intracellular calcium activity in neurons by inhibiting the expression of its downstream target gene her6, which in turn promotes axonal regeneration.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

MicroRNA-9 promotes axon regeneration of mauthner-cell in zebrafish via her6/ calcium activity pathway

Shen,

Chen,

Song

et al. 2024

Cell. Mol. Life Sci.

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“…The accessibility of M-cells for in vivo intracellular recordings provides an excellent opportunity to investigate the cellular mechanisms of multisensory integration. Moreover, the one-to-one relationship between a single M-cell action potential and the initiation of a C-start (Zottoli, 1977; Weiss et al, 2006; Zwaka et al, 2022) provides a clear link between behavior and the observed integration in the M-cell. As such, the goal of this study was to reveal the cellular mechanism that underlie the multimodal integration of M-cell initiated startle escapes we observed in the behavioral experiments (McIntyre and Preuss, 2019; Martorell and Medan, 2022).…”

Section: Introductionmentioning

confidence: 99%

Multisensory integration enhances audiovisual responses in the Mauthner cell

Coronel

Preuss

Medan

2023

Preprint

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Multisensory integration combines information from multiple sensory modalities to create a coherent perception of the world. In contexts where sensory information is limited or equivocal, it also allows animals to integrate individually ambiguous stimuli into a clearer or more accurate percept and, thus, react with a more adaptive behavioral response. Although responses to multisensory stimuli have been described at the neuronal and behavioral levels, a causal or direct link between these two is still missing. In this study, we studied the integration of audiovisual inputs in the Mauthner cell, a command neuron necessary and sufficient to trigger a stereotypical escape response in fish. We performed intracellular recordings while presenting a diverse range of stimuli to determine which stimulus properties affect their integration. Our results show that stimulus modality, intensity, temporal structure, and interstimulus delay affect input summation. Mechanistically, we found that the distinct decay dynamics of feedforward inhibition triggered by auditory and visual stimuli can account for certain aspects of input integration. Altogether, this is the first study to investigate multisensory integration in a cell of clear behavioral relevance and provides a phenomenological and mechanistic characterization of how multisensory integration depends on stimulus properties.

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Days-old zebrafish rapidly learn to recognize threatening agents through noradrenergic and forebrain circuits

Zocchi,

Nguyen,

Marquez-Legorreta

et al. 2024

Current Biology

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Visual object detection biases escape trajectories following acoustic startle in larval zebrafish

Cited by 11 publications

References 47 publications

MicroRNA-9 promotes axon regeneration of mauthner-cell in zebrafish via her6/ calcium activity pathway

MicroRNA-9 promotes axon regeneration of mauthner-cell in zebrafish via her6/ calcium activity pathway

Multisensory integration enhances audiovisual responses in the Mauthner cell

Days-old zebrafish rapidly learn to recognize threatening agents through noradrenergic and forebrain circuits

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