2014
DOI: 10.1089/bio.2014.0011
|View full text |Cite
|
Sign up to set email alerts
|

Visual Observation of African Trypanosomes During Cryopreservation

Abstract: Protozoa have been widely used for the study of cryopreservation. The survival rate after cryopreservation has always received the most attention, while the cell viability during the process of freezing and thawing has been much less studied. In the present study, we report successful cryopreservation of Trypanosoma brucei, a parasitic protozoa of human and animals, using controlled-rate freezing at 5°C/min, and real-time observation of activity using a microscope differential scanning calorimeter system durin… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
5
0

Year Published

2016
2016
2024
2024

Publication Types

Select...
2

Relationship

1
1

Authors

Journals

citations
Cited by 2 publications
(5 citation statements)
references
References 25 publications
0
5
0
Order By: Relevance
“…Although 0.4 M trehalose provided the highest survival capacity of T. brucei in frozen blood of as reported in our previous studies (Wang et al, 2014), we also evaluated the cryoprotective effect of CPA cocktails for trypanosomes in blood. The motility of trypanosomes in blood was almost the same after cryopreservation in all CPAs and CPA cocktails (P> 0.05) (Fig.…”
Section: Optimal Cryoprotectants For Trypanosomes In Bloodmentioning
confidence: 90%
See 4 more Smart Citations
“…Although 0.4 M trehalose provided the highest survival capacity of T. brucei in frozen blood of as reported in our previous studies (Wang et al, 2014), we also evaluated the cryoprotective effect of CPA cocktails for trypanosomes in blood. The motility of trypanosomes in blood was almost the same after cryopreservation in all CPAs and CPA cocktails (P> 0.05) (Fig.…”
Section: Optimal Cryoprotectants For Trypanosomes In Bloodmentioning
confidence: 90%
“…Over dose of trehalose may lead to dehydration resulting in cell death (Fowler & Toner, 2005). We previously found that at concentrations of more than 0.4 M of trehalose, T. brucei motility was significantly impaired (Wang et al, 2014). In order to avoid such damage, a combination strategy using two or more CPAs, known as CPA cocktails, could help to reduce the required amount of each corresponding CPA.…”
Section: Discussionmentioning
confidence: 99%
See 3 more Smart Citations