Visualisation of intramural coronary vasculature by an imaging cryomicrotome suggests compartmentalisation of myocardial perfusion areas

Spaan, Jos A. E.; Wee, Rene ter; Teeffelen, Jurgen W. G. E. van; Streekstra, Geert J.; Siebes, Maria; Kolyva, Christina; Vink, Hans; Fokkema, Dirk S.; VanBavel, Ed

doi:10.1007/bf02344722

Cited by 87 publications

(101 citation statements)

References 14 publications

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“…The experimental approach was the three-dimensional (3D) reconstruction of coronary vascular beds in a porcine model with slowly developing LCX occlusion via an ameroid constrictor. These reconstructions were made from a stack of images obtained by a novel imaging cryomicrotome (27,30), and collaterals were identified by a dedicated suite of imageprocessing software. An important novel finding is the identification of small collaterals within perfusion territories that likely developed in the absence of increased FSS.…”

mentioning

confidence: 99%

Porcine coronary collateral formation in the absence of a pressure gradient remote of the ischemic border zone

Wijngaard

Schulten

Horssen

et al. 2011

American Journal of Physiology-Heart and Circulatory Physiology

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van den Wijngaard JP, Schulten H, van Horssen P, ter Wee RD, Siebes M, Post MJ, Spaan JA. Porcine coronary collateral formation in the absence of a pressure gradient remote of the ischemic border zone. Am J Physiol Heart Circ Physiol 300: H1930 -H1937, 2011. First published March 11, 2011 doi:10.1152/ajpheart.00403.2010.-In the current paradigm on coronary collateral development, it is assumed that these vessels develop consequentially from increased fluid shear stress (FSS) through preexisting collateral arteries. The increased FSS follows from an increase in pressure gradient between the region at risk and well-perfused surroundings. The objective of this study was to test the hypothesis that, in the heart, collateral connections can form in the absence of an increased FFS and consequentially at any depth and region within the ventricular wall. In Yorkshire pigs, gradual left circumflex coronary artery occlusion was obtained over 6 wk by an ameroid constrictor, whereas the control group underwent a sham operation. Hearts were harvested and subsequently processed in an imaging cryomicrotome, resulting in 40-m voxel resolution threedimensional reconstructions of the intramural vascular vessels. Dedicated software segmented the intramural vessels and all continuous vascular pathways containing a collateral connection. In the ameroid group, 192 collaterals, 22-1,049 m in diameter, were detected with 62% within the subendocardium. Sixty percent of collaterals bridged from the left anterior descending artery to left circumflex coronary artery. A novel result is that 25% (n ϭ 48) of smaller-radius collaterals (P ϭ 0.047) connected with both origin and terminus in the nontarget area where perfusion was assumed uncompromised. In the porcine heart, collateral vessels develop not only in ischemic border zones with increased FSS but also away from such border zones where increased FSS is unlikely. The majority of collaterals were located at the subendocardium, corresponding to the region with highest prevalence for ischemia. imaging cryomicrotome; collaterals; ischemia; remodeling; fluid shear stress STIMULATING CORONARY COLLATERAL formation by neovascularization may serve as a last therapeutic resort for patients that cannot be successfully revascularized by coronary artery bypass surgery or percutaneous coronary intervention. However, further development of this therapeutic modality requires more detailed information on the mechanisms of collateral formation.There are differences of opinion whether in the porcine heart collaterals grow de novo, i.e., through angiogenesis stimulated by ischemia, or from innate nonfunctional collaterals that are already present, i.e., arteriogenesis stimulated by shear stress (23,29). In the hindlimb occlusion model, collaterals growing in the nonischemic area proximal to the experimentally induced flow obstruction clearly indicates the role of increased fluid shear stress (FSS) in outward remodeling (7, 9, 23). In the heart, however, regions of ischemia are intertwined with areas of inc...

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mentioning

confidence: 99%

Porcine coronary collateral formation in the absence of a pressure gradient remote of the ischemic border zone

Wijngaard

Schulten

Horssen

et al. 2011

American Journal of Physiology-Heart and Circulatory Physiology

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“…Although no single modality is currently capable of capturing the complete human coronary vasculature, the resolution and acquisition volume of these devices are constantly improving. Notable examples include the micro-computed tomography (mCT), which can image a whole-rat organ vasculature at approximately 1 mm resolution (using a synchrotron X-ray source; Jorgensen et al 1998;Plouraboue et al 2004;Heinzer et al 2006); a custom-built cryomicrotome device that captures serially sectioned images of a whole human heart at approximately 25 mm resolution (Spaan et al 2005); and the extended volume confocal imaging device, capable of distinguishing multiple tissue types (Sands et al 2005). Although these modalities are applied destructively on tissue samples, combining them with functional imaging studies provides a possible means to deal with the problems of model parametrization and in vivo verification.…”

Section: Coronary Blood Flowmentioning

confidence: 99%

Coupling contraction, excitation, ventricular and coronary blood flow across scale and physics in the heart

Lee

Niederer

Nordsletten

et al. 2009

Phil. Trans. R. Soc. A.

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In this study, we review the development and application of multi-physics and multiscale coupling in the construction of whole-heart physiological models. Through an examination of recent computational modelling developments, we analyse the significance of coupling mechanisms for the increased understanding of cardiac function in the areas of excitation-contraction, coronary blood flow and ventricular fluid mechanical coupling. Within these physiological domains, we demonstrate and discuss the importance of model parametrization, imaging-based model anatomy and computational implementation.

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“…Frozen hearts were immersed in a cylindrical container containing 5% carboxymethylcellulose sodium solvent supplemented with Indian ink and frozen at Ϫ20°C. All samples were processed with a custom-built automated imaging cryomicrotome (26,27). Hearts were alternately cut at 14-m slice thickness and the block face was imaged using a 16-bit cooled CCD camera (Apogee Alta U-16) with an adjustable focus lens (Nikon 70 -180 mm).…”

Section: Methodsmentioning

confidence: 99%

Selective subepicardial localization of monocyte subsets in response to progressive coronary artery constriction

Hakimzadeh

Lier

Horssen

et al. 2016

American Journal of Physiology-Heart and Circulatory Physiology

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Hakimzadeh N, van Lier MG, van Horssen P, Daal M, Ly DH, Belterman C, Coronel R, Spaan JA, Siebes M. Selective subepicardial localization of monocyte subsets in response to progressive coronary artery constriction. Am J Physiol Heart Circ Physiol 311: H239-H250, 2016. First published May 20, 2016 doi:10.1152/ajpheart.00187.2016.-Following myocardial infarction and atherosclerotic lesion development, monocytes contribute to myocardial protection and repair, while also partaking in myocardial ischemic injury. The balance of proinflammatory and reparative monocyte subsets is crucial in governing these therapeutic and pathological outcomes. Myocardial ischemic damage displays heterogeneity across the myocardium, whereby the subendocardium shows greatest vulnerability to ischemic damage. In this study we examined the transmural distribution of monocyte subsets in response to gradual coronary artery occlusion. CD14 ϩ monocytes were isolated from peripheral blood of New Zealand White rabbits and divided into two subgroups based on the expression of CD62L. We employed a rabbit model of progressive coronary artery obstruction to induce chronic myocardial ischemia and reinfused fluorescently labeled autologous monocytes. The distribution of fluorescently labeled autologous monocytes was examined with a high-resolution three-dimensional imaging cryomicrotome. The subepicardial layer contained the largest infiltration of both monocyte subgroups, with a significantly greater proportion of CD14 ϩ CD62L ϩ monocytes at the time when the ischemic area was at a maximum. By targeting CD13 ϩ angiogenic vessels, we confirmed the presence of angiogenesis in epicardial and midmyocardial regions. These myocardial regions demonstrated the highest level of infiltration of both monocyte subsets. Furthermore, CD14 ϩ CD62L ϩ monocytes showed significantly greater migration towards monocyte chemoattractant protein-1, greater adhesive capacity, and higher expression of C-C chemokine receptor type-2 relative to CD14 ϩ CD62L Ϫ monocytes. In conclusion, we note selective subepicardial distribution of monocyte subpopulations, with changes in proportion depending on the time after onset of coronary narrowing. Selective homing is supported by divergent migratory properties of each respective monocyte subgroup.

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Visualisation of intramural coronary vasculature by an imaging cryomicrotome suggests compartmentalisation of myocardial perfusion areas

Cited by 87 publications

References 14 publications

Porcine coronary collateral formation in the absence of a pressure gradient remote of the ischemic border zone

Porcine coronary collateral formation in the absence of a pressure gradient remote of the ischemic border zone

Coupling contraction, excitation, ventricular and coronary blood flow across scale and physics in the heart

Selective subepicardial localization of monocyte subsets in response to progressive coronary artery constriction

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