Visualisation of mycorrhizal fungal structures and quantification of their surface area and volume using laser scanning confocal microscopy

Dickson, S.; Kolesik, Peter

doi:10.1007/s005720050268

Cited by 79 publications

(32 citation statements)

References 24 publications

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“…The volume and surface area estimates of these arbuscules are in the same range as those of arbuscules of Glomus coronatum (formally 'G. City Beach') measured in Allium porrum (Dickson 1999;Dickson and Kolesik 1999).…”

Section: Discussionmentioning

confidence: 52%

“…Collapsed arbuscules have been shown to autofluoresce strongly (Ames et al 1982;Vierheilig et al 1999Vierheilig et al , 2001). However, acid fuchsin staining has also been used to produce bright fluorescence under confocal conditions (Merryweather and Fitter 1991;Dickson and Kolesik 1999). The differences in fluorescence in the paired arbuscules in this study may be due to the lower uptake of acid fuchsin in active structures because of prestaining with succinate dehydrogenase solution or due to fluorescence quenching by the formazan precipitate.…”

Section: Discussionmentioning

confidence: 93%

“…Most images were further magnified by electronic zoom. A series of optical xyslices were collected at 1-µm intervals, which was corrected to 0.71 µm to allow for axial distortion of the immersion and mounting media (see Dickson and Kolesik 1999). Confocal images were recorded and stored as Leica files.…”

Section: Methodsmentioning

confidence: 99%

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Paired arbuscules in the Arum-type arbuscular mycorrhizal symbiosis with Linum usitatissimum

Dickson¹,

Schweiger²,

Smith³

et al. 2003

Can. J. Bot.

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Experiments were conducted to investigate the "paired" arbuscules characteristic of Arum-type mycorrhizal colonization in Linum usitatissimum L. The development and senescence of arbuscular structures were followed in a time course study. Roots were freeze-sectioned longitudinally and mycorrhizal structures visualized using nitroblue tetrazolium, a vital stain to indicate metabolically active arbuscules and intercellular hyphae, followed by acid fuchsin counterstaining. Arbuscules were imaged using laser scanning confocal microscopy. The volume and surface area of each arbuscule of a developing paired structure were measured using three-dimensional imaging software. Arbuscules occurred in pairs in adjacent cortical cells arising from a single, radial intercellular hypha. These "paired" arbuscules often appeared to be at different developmental stages. Logistic regression and measurement of surface area indicated that there was a delay in initiation of the second arbuscule.

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 93%

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Paired arbuscules in the Arum-type arbuscular mycorrhizal symbiosis with Linum usitatissimum

Dickson¹,

Schweiger²,

Smith³

et al. 2003

Can. J. Bot.

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“…Hickey et al (2005) have given a review of livecell imaging of filamentous fungi using vital fluorescent dyes and confocal microscopy. Dickson & Kolesik (1999) also used confocal microscopy for the visualisation of mycorrhizal fungal structures and quantification of their surface area and volume. Muller et al (2001Muller et al ( , 2002 employed magnetic resonance imaging for the detection of incipient fungal attack in wood.…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional X-Ray Imaging and Analysis of Fungi on and in Wood

et al. 2009

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As wood is prone to fungal degradation, fundamental research is necessary to increase our knowledge aiming at product improvement. Several imaging modalities are capable of visualizing fungi, but the X-ray equipment presented in this paper can envisage fungal mycelium in wood non-destructively in three dimensions with sub-micron resolution. Four types of wood subjected to the action of the white rot fungus Coriolus versicolor (Linnaeus) Quélet (CTB 863 A) were scanned using an X-ray based approach. Comparison of wood volumes before and after fungal exposure, segmented manually or semi-automatically, showed the presence of the fungal mass on and in the wood samples and therefore demonstrated the usefulness of computed X-ray tomography for mycological and wood research. Further improvements to the experimental set-up are necessary in order to resolve individual hyphae and enhance segmentation.2

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“…Recently, the use of a laser scanning confocal microscope (LSCM) resulted in images of the 3D structure of arbuscular mycorrhizal structures of much higher resolution than possible with conventional light microscopical techniques (Dickson & Kolesik 1999).…”

Section: Introductionmentioning

confidence: 99%

Visualisation of ectomycorrhizal rhizomorph structure using laser scanning confocal microscopy

Schweiger

Rouhier

Söderström

2002

Mycological Research

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A method for the observation of the three-dimensional structure of intact ectomycorrhizal rhizomorphs is described. The method is based on a combination of clearing the material with KOH followed by staining with congo red and subsequent imaging under a laser scanning confocal microscope (LSCM). The images obtained are of a much higher three-dimensional resolution than those obtained previously by use of conventional light microscopical techniques. The structure of highly differentiated and undifferentiated rhizomorphs is described. Applications of the method are briefly discussed.

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Visualisation of mycorrhizal fungal structures and quantification of their surface area and volume using laser scanning confocal microscopy

Cited by 79 publications

References 24 publications

Paired arbuscules in the Arum-type arbuscular mycorrhizal symbiosis with Linum usitatissimum

Paired arbuscules in the Arum-type arbuscular mycorrhizal symbiosis with Linum usitatissimum

Three-Dimensional X-Ray Imaging and Analysis of Fungi on and in Wood

Visualisation of ectomycorrhizal rhizomorph structure using laser scanning confocal microscopy

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