Visualisation of plastid outgrowths in potato (Solanum tuberosum L.) tubers by carboxyfluorescein diacetate staining

Borucki, Wojciech; Bederska, Magdalena; Sujkowska-Rybkowska, Marzena

doi:10.1007/s00299-015-1748-2

Cited by 8 publications

(3 citation statements)

References 23 publications

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“…In order to determine whether the accumulation of crocins was accompanied by changes at the structural level, the storage parenchyma cells of potato tubers of line O6 8E were analyzed. Large amyloplasts containing starch grains are prominent features of these potato tuber cells ( 68 ). An overview of the structure of these cells is presented in Figure 3 , where different cell compartments are visualized, as mitochondria, peroxisomes, vacuoles, and endoplasmic reticulum ( Figures 3A,B ).…”

Section: Resultsmentioning

confidence: 99%

Fortification and bioaccessibility of saffron apocarotenoids in potato tubers

Gómez

Morote²,

Frusciante³

et al. 2022

Front. Nutr.

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Carotenoids are C40 isoprenoids with well-established roles in photosynthesis, pollination, photoprotection, and hormone biosynthesis. The enzymatic or ROS-induced cleavage of carotenoids generates a group of compounds named apocarotenoids, with an increasing interest by virtue of their metabolic, physiological, and ecological activities. Both classes are used industrially in a variety of fields as colorants, supplements, and bio-actives. Crocins and picrocrocin, two saffron apocarotenoids, are examples of high-value pigments utilized in the food, feed, and pharmaceutical industries. In this study, a unique construct was achieved, namely O6, which contains CsCCD2L, UGT74AD1, and UGT709G1 genes responsible for the biosynthesis of saffron apocarotenoids driven by a patatin promoter for the generation of potato tubers producing crocins and picrocrocin. Different tuber potatoes accumulated crocins and picrocrocin ranging from 19.41–360 to 105–800 μg/g DW, respectively, with crocetin, crocin 1 [(crocetin-(β-D-glucosyl)-ester)] and crocin 2 [(crocetin)-(β-D-glucosyl)-(β-D-glucosyl)-ester)] being the main compounds detected. The pattern of carotenoids and apocarotenoids were distinct between wild type and transgenic tubers and were related to changes in the expression of the pathway genes, especially from PSY2, CCD1, and CCD4. In addition, the engineered tubers showed higher antioxidant capacity, up to almost 4-fold more than the wild type, which is a promising sign for the potential health advantages of these lines. In order to better investigate these aspects, different cooking methods were applied, and each process displayed a significant impact on the retention of apocarotenoids. More in detail, the in vitro bioaccessibility of these metabolites was found to be higher in boiled potatoes (97.23%) compared to raw, baked, and fried ones (80.97, 78.96, and 76.18%, respectively). Overall, this work shows that potatoes can be engineered to accumulate saffron apocarotenoids that, when consumed, can potentially offer better health benefits. Moreover, the high bioaccessibility of these compounds revealed that potato is an excellent way to deliver crocins and picrocrocin, while also helping to improve its nutritional value.

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Section: Resultsmentioning

confidence: 99%

Fortification and bioaccessibility of saffron apocarotenoids in potato tubers

Gómez

Morote²,

Frusciante³

et al. 2022

Front. Nutr.

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“…According to the proposed model stressed-induced autophagy sequesters stromules by forming an isolation membrane that eventually clips off a given stromule and its stromal contents, and then transports the cargo to the vacuole for degradation ( Ishida et al, 2008 ). Interestingly, both stromules and protrusions were identified in potato tuber amyloplasts ( Borucki et al, 2015 ). These protrusions, unlike stromules, are likely involved in starch accumulation in the parenchyma storage cells.…”

Section: Chloroplasts and Inter-organellar Signalingmentioning

confidence: 99%

Chloroplast signaling within, between and beyond cells

2015

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The most conspicuous function of plastids is the oxygenic photosynthesis of chloroplasts, yet plastids are super-factories that produce a plethora of compounds that are indispensable for proper plant physiology and development. Given their origins as free-living prokaryotes, it is not surprising that plastids possess their own genomes whose expression is essential to plastid function. This semi-autonomous character of plastids requires the existence of sophisticated regulatory mechanisms that provide reliable communication between them and other cellular compartments. Such intracellular signaling is necessary for coordinating whole-cell responses to constantly varying environmental cues and cellular metabolic needs. This is achieved by plastids acting as receivers and transmitters of specific signals that coordinate expression of the nuclear and plastid genomes according to particular needs. In this review we will consider the so-called retrograde signaling occurring between plastids and nuclei, and between plastids and other organelles. Another important role of the plastid we will discuss is the involvement of plastid signaling in biotic and abiotic stress that, in addition to influencing retrograde signaling, has direct effects on several cellular compartments including the cell wall. We will also review recent evidence pointing to an intriguing function of chloroplasts in regulating intercellular symplasmic transport. Finally, we consider an intriguing yet less widely known aspect of plant biology, chloroplast signaling from the perspective of the entire plant. Thus, accumulating evidence highlights that chloroplasts, with their complex signaling pathways, provide a mechanism for exquisite regulation of plant development, metabolism and responses to the environment. As chloroplast processes are targeted for engineering for improved productivity the effect of such modifications on chloroplast signaling will have to be carefully considered in order to avoid unintended consequences on plant growth and development.

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“…Stromules were named in 2000 in order to distinguish the structures from other types of tubular structures in the cell (Köhler and Hanson, 2000). Since then, stromules have been observed through fluorescent labeling in a wide variety of plant species and cell types (for review, see Gray et al, 2001;Kwok and Hanson, 2004a;Natesan et al, 2005;Hanson and Sattarzadeh, 2008;Borucki et al, 2015). Stromules are implicated as structures that function in a large variety of cellular activities (Box 1).…”

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confidence: 99%