Visualization of active biomass distribution in a BGAC fluidized bed reactor using GFP tagged Pseudomonas putida F1

Herzberg, Moshe; Dosoretz, Carlos G.; Kuhn, Jonathan; Klein, Shiri; Green, Michal

doi:10.1016/j.watres.2006.05.006

Cited by 21 publications

(10 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The biofilm thicknesses are similar to those found by Hwang et al (22) for OSPW biofilms produced on polyethylene (PE) and polyvinylchloride (PVC) coupons in continuous bioreactors, where a continuous (full covered) biofilm on the surface was observed on these relatively smooth surfaces. This is expected, as the bacteria form a complex structure of aerobic biofilms formed by discrete aggregates of densely packed cells and interstitial voids on the GAC surfaces as reported previously (38,39). The ozonated OSPW treatment showed faster initial attachment of bacteria (by day 18), despite having a biofilm thickness similar to that of raw OSPW after 48 days.…”

Section: Methodssupporting

confidence: 78%

“…The GAC biofilm thicknesses were greater than the previously observed biofilm thickness (34 Ϯ 5 m) for the treatment of OSPW using fluidized bed biofilm reactors (15,16). The greater biofilm thickness on the GAC from the current study might be attributed to a reduction in biofilm detachment from the lower shear stress in the batch reactors compared with that in the fluidized bed biofilm reactors (39,41).…”

Section: Methodscontrasting

confidence: 67%

See 1 more Smart Citation

Next-Generation Pyrosequencing Analysis of Microbial Biofilm Communities on Granular Activated Carbon in Treatment of Oil Sands Process-Affected Water

Islam

Zhang

McPhedran

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

The development of biodegradation treatment processes for oil sands process-affected water (OSPW) has been progressing in recent years with the promising potential of biofilm reactors. Previously, the granular activated carbon (GAC) biofilm process was successfully employed for treatment of a large variety of recalcitrant organic compounds in domestic and industrial wastewaters. In this study, GAC biofilm microbial development and degradation efficiency were investigated for OSPW treatment by monitoring the biofilm growth on the GAC surface in raw and ozonated OSPW in batch bioreactors. The GAC biofilm community was characterized using a next-generation 16S rRNA gene pyrosequencing technique that revealed that the phylum Proteobacteria was dominant in both OSPW and biofilms, with further in-depth analysis showing higher abundances of Alpha-and Gammaproteobacteria sequences. Interestingly, many known polyaromatic hydrocarbon degraders, namely, Burkholderiales, Pseudomonadales, Bdellovibrionales, and Sphingomonadales, were observed in the GAC biofilm. Ozonation decreased the microbial diversity in planktonic OSPW but increased the microbial diversity in the GAC biofilms. Quantitative real-time PCR revealed similar bacterial gene copy numbers (>10 9 gene copies/g of GAC) for both raw and ozonated OSPW GAC biofilms. The observed rates of removal of naphthenic acids (NAs) over the 2-day experiments for the GAC biofilm treatments of raw and ozonated OSPW were 31% and 66%, respectively. Overall, a relatively low ozone dose (30 mg of O 3 /liter utilized) combined with GAC biofilm treatment significantly increased NA removal rates. The treatment of OSPW in bioreactors using GAC biofilms is a promising technology for the reduction of recalcitrant OSPW organic compounds.O pen-pit mining of Canada's Athabasca oil sands produces large quantities of bitumen coated sands. The bitumen is extracted from the sands using the Clark hot-water process, in which 1 m 3 of bitumen extracted from oil sands generates 4 m 3 of oil sands process-affected water (OSPW) (1-3). Over a billion cubic meters of this generated OSPW is currently stored in tailing ponds given the no-release policy for OSPW followed by the oil sands companies (4, 5). This volume of OSPW will continually increase as the oil sands bitumen extraction continues in the Athabasca region until suitable treatment technologies become available to allow for OSPW release (2). This OSPW is contaminated with a large number of inorganic and organic compounds during the extraction process that are known to be toxic to aquatic and terrestrial life (6). Thus, the production of OSPW associated with the extraction of oil sands bitumen is a great environmental issue that needs to be addressed in the near future for the remediation of the oil sands region.Fresh OSPW has acute, subchronic, and chronic toxicities to aquatic organisms, and the majority of this toxicity has been previously attributed to naphthenic acids (NAs) (2, 7). NAs are a mixture of organic surfactants containing carboxy...

show abstract

Section: Methodssupporting

confidence: 78%

Section: Methodscontrasting

confidence: 67%

Next-Generation Pyrosequencing Analysis of Microbial Biofilm Communities on Granular Activated Carbon in Treatment of Oil Sands Process-Affected Water

Islam

Zhang

McPhedran

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…A specific form of activated carbon, granular activated carbon (GAC), consists of irregularly shaped particles having a porous structure which can both absorb (into microorganism biofilm) and adsorb (directly) many chemical compounds. The porous structure can protect microorganisms from potentially high fluid shear forces, thus promoting microbial colonization which can lead to biodegradation (Herzberg et al 2006). In addition, GAC has a high adsorption capacity, due to a large specific surface area, which increases the availability of organic substrates and nutrients to microorganisms at the media surface and, subsequently enhances biodegradation efficiency (Herzberg et al 2006).…”

Section: Introductionmentioning

confidence: 99%

“…The porous structure can protect microorganisms from potentially high fluid shear forces, thus promoting microbial colonization which can lead to biodegradation (Herzberg et al 2006). In addition, GAC has a high adsorption capacity, due to a large specific surface area, which increases the availability of organic substrates and nutrients to microorganisms at the media surface and, subsequently enhances biodegradation efficiency (Herzberg et al 2006). The ability of GAC to be bioregenerated in biofilm reactors acts to enhance organics removal well beyond only the adsorption capacity which would normally be exhausted and need to be regenerated in the absence of this bioregeneration (Aktas and Cecen 2007).…”

Section: Introductionmentioning

confidence: 99%

Impact of ozonation pre-treatment of oil sands process-affected water on the operational performance of a GAC-fluidized bed biofilm reactor

et al. 2014

View full text Add to dashboard Cite

Treatment of oil sands process-affected water (OSPW) using biodegradation has the potential to be an environmentally sound approach for tailings water reclamation. This process is both economical and efficient, however, the recalcitrance of some OSPW constituents, such as naphthenic acids (NAs), require the pre-treatment of raw OSPW to improve its biodegradability. This study evaluated the treatment of OSPW using ozonation followed by fluidized bed biofilm reactor (FBBR) using granular activated carbon (GAC). Different organic and hydraulic loading rates were applied to investigate the performance of the bioreactor over 120 days. It was shown that ozonation improved the adsorption capacity of GAC for OSPW and improved biodegradation by reducing NAs cyclicity. Bioreactor treatment efficiencies were dependent on the organic loading rate (OLR), and to a lesser degree, the hydraulic loading rate (HLR). The combined ozonation, GAC adsorption, and biodegradation process removed 62 % of chemical oxygen demand (COD), 88 % of acid-extractable fraction (AEF) and 99.9 % of NAs under optimized operational conditions. Compared with a planktonic bacterial community in raw and ozonated OSPW, more diverse microbial communities were found in biofilms colonized on the surface of GAC after 120 days, with various carbon degraders found in the bioreactor including Burkholderia multivorans, Polaromonas jejuensis and Roseomonas sp.

show abstract

“…Others. Green fluorescent protein (GFP) was used as a marker on a phenol degrading bacterium, Pseudomonas putida F1, to monitor biofilm growth (Herzberg et al, 2006). Quantitative analysis of the biofilm, by using COMSTAT image-processing software, showed higher biomass concentration and higher viability in the granular activated carbon (GAC) covered biofilm vs. the non-activated carbon biofilm.…”

Section: Time Of Flight-secondary Ion Mass Spectrometrymentioning

confidence: 99%

Biological Fixed Film Systems

Sharma¹,

McSwain²,

Zhang³

et al. 2007

Water Environment Research

View full text Add to dashboard Cite

Visualization of active biomass distribution in a BGAC fluidized bed reactor using GFP tagged Pseudomonas putida F1

Cited by 21 publications

References 19 publications

Next-Generation Pyrosequencing Analysis of Microbial Biofilm Communities on Granular Activated Carbon in Treatment of Oil Sands Process-Affected Water

Next-Generation Pyrosequencing Analysis of Microbial Biofilm Communities on Granular Activated Carbon in Treatment of Oil Sands Process-Affected Water

Impact of ozonation pre-treatment of oil sands process-affected water on the operational performance of a GAC-fluidized bed biofilm reactor

Biological Fixed Film Systems

Contact Info

Product

Resources

About