Visualization of carious lesions with polarized and depolarized light microscopy

Grundmann, Julia; Golde, Jonas; Steuer, Svea; Tetschke, Florian; Kirsten, Lars; Walther, Julia; Koch, Edmund; Hannig, Christian

doi:10.1364/boe.514904

Cited by 4 publications

(1 citation statement)

References 48 publications

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“…Since its inception, optical coherence tomography (OCT) has undergone remarkable progress, evolving from time-domain to spectral-domain configurations and, more recently, embracing the sophisticated swept-source OCT (SS-OCT) [ 1 – 3 ]. These continuous technological innovations have firmly established OCT as an indispensable tool in various biomedical imaging applications, spanning clinical fields such as ophthalmology [ 4 – 7 ], dentistry [ 8 – 12 ], dermatology [ 13 – 17 ], endoscopy [ 18 – 23 ], neuroscience [ 24 – 27 ], and more.…”

Section: Introductionmentioning

confidence: 99%

High-speed, long-range and wide-field OCT for in vivo 3D imaging of the oral cavity achieved by a 600 kHz swept source laser

Shi,

Liu,

Wang

2024

Biomed. Opt. Express

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We report a high-speed, long-range, and wide-field swept-source optical coherence tomography (SS-OCT) system aimed for imaging microstructures and microcirculations in the oral cavity. This system operates at a scan speed of 600 kHz, delivering a wide imaging field of view at 42 × 42 mm2 and a ranging distance of 36 mm. To simultaneously meet the requirements of high speed and long range, it is necessary for the k-clock trigger signal to be generated at its maximum speed, which may induce non-linear phase response in electronic devices due to the excessive k-clock frequency bandwidth, leading to phase errors. To address this challenge, we introduced a concept of electrical dispersion and a global k-clock compensation approach to improve overall performance of the imaging system. Additionally, image distortion in the wide-field imaging mode is also corrected using a method based on distortion vector maps. With this system, we demonstrate comprehensive structural and blood flow imaging of the anterior oral cavity in healthy individuals. The high-speed, long-range, and wide-field SS-OCT system opens new opportunities for comprehensive oral cavity examinations and holds promise as a reliable tool for assessing oral health conditions.

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Section: Introductionmentioning

confidence: 99%

High-speed, long-range and wide-field OCT for in vivo 3D imaging of the oral cavity achieved by a 600 kHz swept source laser

Shi,

Liu,

Wang

2024

Biomed. Opt. Express

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Optical properties of dentin and enamel by spectroscopic ellipsometry

Castilho,

Nunes,

Barroso

et al. 2025

Optik

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Topographic Evaluation of the Remineralizing Potential of Biomimetic Scaffolds on Enamel White Spot Lesions: An In Vitro Study

Soliman,

Abdelfattah,

Mohamed

et al. 2024

J Esthet Restor Dent

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ObjectivesThe modern approach to managing noncavitated white spot lesions (WSLs) emphasizes noninvasive strategies and biomimetic remineralization. Biomimetic scaffolds are designed to regenerate dental tissues rather than simply repair them. This study aimed to assess lesion depth, enamel structure, and the elemental composition of artificially induced WSLs after treatment with biomimetic remineralization techniques.Materials and MethodsNinety‐six freshly extracted anterior teeth, free from caries or enamel defects and extracted due to periodontal disease, were used. White spot lesions were induced on the labial surfaces, and the samples were divided into three groups based on the remineralizing agent: Group I, treated with casein phosphopeptide‐amorphous calcium phosphate (CPP‐ACP), which served as the positive control; Group II, treated with self‐assembling peptide P11‐4 (SAP‐P11‐4); and Group III, treated with phosphorylated nano‐chitosan (Pchi‐ACP). Enamel topography was analyzed at baseline and after treatment using polarized light microscopy, micro‐Raman spectroscopy, and scanning electron microscopy. Statistical analysis was conducted using one‐way ANOVA, Kruskal–Wallis, and Dunn's post hoc test (p = 0.05).ResultsThe Kruskal–Wallis test indicated a significant difference in remineralization potential among the groups. Pchi‐ACP showed the greatest reduction in lesion depth (62.65%), demonstrating significant subsurface enamel remineralization. This group also exhibited a smooth, regular enamel surface with shallow linear depressions. Elemental analysis confirmed successful calcium phosphate precipitation in Pchi‐ACP, indicating a trend toward enamel regeneration.ConclusionPchi‐ACP represents a promising biomimetic and minimally invasive approach for treating early WSLs, signifying a transition toward regenerative dentistry. (SAP‐P11‐4), while effective, was less successful than phosphorylated nano‐chitosan but outperformed (CPP‐ACP).Clinical SignificancePchi‐ACP demonstrates significant potential for minimally invasive treatment of early noncavitated carious lesions. By preserving natural tooth structure, this approach could greatly enhance oral health outcomes in the long term.

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Visualization of carious lesions with polarized and depolarized light microscopy

Cited by 4 publications

References 48 publications

High-speed, long-range and wide-field OCT for in vivo 3D imaging of the oral cavity achieved by a 600 kHz swept source laser

High-speed, long-range and wide-field OCT for in vivo 3D imaging of the oral cavity achieved by a 600 kHz swept source laser

Optical properties of dentin and enamel by spectroscopic ellipsometry

Topographic Evaluation of the Remineralizing Potential of Biomimetic Scaffolds on Enamel White Spot Lesions: An In Vitro Study

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