Visualization of Cell Composition and Maturation in the Bone Marrow Using 10‐Color Flow Cytometry and Radar Plots

Abstract

We demonstrated that the cell clusters of normal BM can be mapped on multidimensional radar plots, which provide an inclusive insight into BM cell composition and maturation. These reproducible RPs present a comprehensive and comprehensible visual display of differentiation and maturation of haematopoietic cells in normal BM, and can be used as a reference map to assess abnormal haematopoiesis in MDS. © 2017 International Clinical Cytometry Society.

Clinical Implication of Multi-Parameter Flow Cytometry in Myelodysplastic Syndromes

“…Most visualization tools apply dimensionality reduction to comprehensively visualize multi-dimensional data in a two-dimensional manner. Jafari et al [54] applied a visualization method on MFC data of MDS patients and healthy patients called radar-plots. They showed that this method is able to group cells in accordance with manual gating strategies.…”

Analysis of erythroid maturation in the nonlysed bone marrow with help of radar plots facilitates detection of flow cytometric aberrations in myelodysplastic syndromes

“…We have previously characterized blast region in normal bone marrow and in MDS patients using multivariate radar plots (Jafari et al, 2018). In the present study, we illustrate the normal erythroid maturation on conventional bivariate dot plots and display the normal maturation pattern on RP.…”

“…Both methods can be used to assess distribution of different subsets of the erythroid population, considering optimal acquisition of erythroid lineage using a no-lyse method. RP is a graphical display making it easier to evaluate of the FCM aberrancies (Jafari et al, 2018). We recognized three different abnormal erythropoietic patterns by using RP.…”

Issue Highlights – September 2020

“…Because of the difficulty examining the numerous phenotypes associated with this heterogenous disease investigators have often used a variety of unsupervised clustering algorithms including SPADE, viSNE, and FlowSOM to enhance the diagnostic utility of flow cytometry for MDS (9‐11). Members of this group previously used Kaluza radar plots to reproducibility display the differentiation and maturation of hematopoietic cells in normal bone marrow (12). Here they convincingly demonstrate how radar plots can facilitate the evaluation of erythropoietic maturation patterns.…”