Visualization of Cisplatin-DNA and Carboplatin-DNA Adducts in Tissues and Cultured Cells

Terheggen, P. M. A. B.; Begg, Adrian C.; Floot, Ben; Emondt, J.; Engelse, L. Den

doi:10.1007/978-1-4613-1717-3_17

Cited by 7 publications

(2 citation statements)

References 3 publications

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“…Two-micron sections were cut and mounted on slides, coated with a solution of chromium(III)-potassium sulphate (0.1%) and gelatin (1%). The immunocytochemical procedure was carried out essentially as described by Terheggen et al (1987). Briefly the staining procedure was as follows: sections were treated with methanol-hydrogen peroxide (to inactivate endogenous peroxidase) and ethanol-sodium hydroxide (to denature the DNA and/or to increase the accessibility to antibodies).…”

Section: Tumor Modelmentioning

confidence: 99%

Response of peritoneal solid tumours after intraperitoneal chemohyperthermia treatment with cisplatin or carboplatin

Los

Vugt²,

Pinedo³

1994

Br J Cancer

129

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The combination of heat and chemotherapy was studied in an intraperitoneal tumour model. Rats bearing peritoneal CC531 tumours (2-6 mm) were treated i.p. with cDDP or CBDCA [maximal tolerated dose (MTD)] in combination with regional hyperthermia (41.5 degrees C, 1 h) of the peritoneal cavity. The addition of hyperthermia to the i.p. treatment led to a decrease in the MTD of cDDP by 33.3% at 41.5 degrees C. This was due to increased nephrotoxicity. The MTD of CBDCA did not change as a result of hyperthermia treatment. The chemo-hyperthermia treatment resulted in more cDDP or CBDCA DNA adducts in peritoneal tumours after the combined treatment than after chemotherapy alone. The increased tumour platinum concentrations, rising from 1.3 micrograms Pt g-1 tumour at 37 degrees C to 5.4 micrograms Pt g-1 tumour at 41.5 degrees C for cDDP and from 0.2 microgram Pt g-1 tumor to 0.7 microgram Pt g-1 tumour at 41.5 degrees C for CBDCA, contributed considerably to the enhanced numbers of cDDP or CBDCA DNA adducts. As a result of the latter, i.p. chemotherapy combined with regional hyperthermia led to an increase in tumour growth delay (TGD) after increasing the temperature to 41.5 degrees C for cDDP and CBDCA (by 40 days for cDDP, 22 days for CBDCA). These data were in agreement with the in vitro findings, i.e. that higher temperatures led to increased cytotoxicity.

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Section: Tumor Modelmentioning

confidence: 99%

Response of peritoneal solid tumours after intraperitoneal chemohyperthermia treatment with cisplatin or carboplatin

Los

Vugt²,

Pinedo³

1994

Br J Cancer

129

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“…The high Pt accumulation in skin supports the application of HILP in melanoma. It might be more realistic to express tissue concentrations per g protein or DNA content instead of per tissue weight or to determine the amount of Pt-DNA adducts formed (Terheggen et al, 1988).…”

mentioning

confidence: 99%

Cisplatin and platinum pharmacokinetics during hyperthermic isolated limb perfusion for human tumours of the extremities

Guchelaar¹,

Hoekstra²,

Devries

et al. 1992

Br J Cancer

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Visualization and Computer-Assisted Quantification of DNA Modifications in Individual Cells

Engelse

Ploem

Wild

et al. 1989

DNA Damage and Repair

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Visualization of Cisplatin-DNA and Carboplatin-DNA Adducts in Tissues and Cultured Cells

Cited by 7 publications

References 3 publications

Response of peritoneal solid tumours after intraperitoneal chemohyperthermia treatment with cisplatin or carboplatin

Response of peritoneal solid tumours after intraperitoneal chemohyperthermia treatment with cisplatin or carboplatin

Cisplatin and platinum pharmacokinetics during hyperthermic isolated limb perfusion for human tumours of the extremities

Visualization and Computer-Assisted Quantification of DNA Modifications in Individual Cells

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