Visualization of DNA in highly processed botanical materials

Lu, Zhengfei; Rubinsky, Maria; Babajanian, Silva; Zhang, Yanjun; Chang, Peter; Swanson, Gary

doi:10.1016/j.foodchem.2017.11.067

Cited by 15 publications

(15 citation statements)

References 28 publications

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“…Moreover, the fragments of 836 and 1487 bp were found to be largely degraded after the first ethanol extraction, and almost no fragment of 1487 bp could be observed after the fourth ethanol extraction. Thus, our results confirmed previous findings that DNA in botanicals could be either heavily degraded or completely eliminated upon solvent extraction [24].…”

Section: Dna Fragmentation Along Soya Bean Protein Concentrate Preparsupporting

confidence: 92%

Monitoring and traceability of genetically modified soya bean event GTS 40-3-2 during soya bean protein concentrate and isolate preparation

Chen

et al. 2020

R. Soc. open sci.

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To evaluate DNA fragmentation and GMO quantification during soya bean protein concentrate and isolate preparation, genetically modified soya bean event GTS 40-3-2 (Roundup Ready TM soya bean, RRS) was blended with conventional soya beans at mass percentages of 0.9%, 2%, 3%, 5% and 10%. Qualitative PCR and real-time PCR were used to monitor the taxon-specific lectin and exogenous cp4 epsps target levels in all of the main products and by-products, which has practical significance for RRS labelling threshold and traceability. Along the preparation chain, the majority of DNA was distributed in main products, and the DNA degradation was noticed. From a holistic perspective, the lectin target degraded more than cp4 epsps target during both of the two soya bean proteins preparations. Therefore, the transgenic contents in the final protein products were higher than the actual mass percentages of RRS in raw materials. Our results are beneficial to the improvement of GMO labelling legislation and the protection of consumer rights.

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Section: Dna Fragmentation Along Soya Bean Protein Concentrate Preparsupporting

confidence: 92%

Monitoring and traceability of genetically modified soya bean event GTS 40-3-2 during soya bean protein concentrate and isolate preparation

Chen

et al. 2020

R. Soc. open sci.

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“…Raw materials have sufficient DNA that is of good quality for both DNA barcoding and in some cases NGS. This claim is supported by several studies (Cheng et al., 2015; Gao et al., 2017; Han et al., 2016; Lu et al., 2018; Newmaster et al., 2013; Palhares et al., 2015; Raclariu et al., 2017; Seethapathy et al., 2015; Shanmughanandhan et al., 2016) that have demonstrated the ability to recover high quality DNA sequences from raw materials. Some industry members have postulated that highly processed NHPs may not have any DNA present.…”

Section: Introductionmentioning

confidence: 65%

“…Some industry members have postulated that highly processed NHPs may not have any DNA present. Recent research by Lu et al. (2018) demonstrated the ability to visualize DNA in highly processed botanicals using adapter-ligation and PCR amplification methods.…”

Section: Introductionmentioning

confidence: 99%

Exploring DNA quantity and quality from raw materials to botanical extracts

Ragupathy

Faller

Dhivya

et al. 2019

Heliyon

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Objectives The aim of this study was to explore the variability in DNA quality and quantity along a gradient of industrial processing of botanical ingredients from raw materials to extracts. Methods A data matrix was assembled for 1242 botanical ingredient samples along a gradient of industrial processing commonly used in the Natural Health Product (NHP) industry. Multivariate statistics was used to explore dependant variables for quality and quantity. The success of attaining a positive DNA test result along a gradient of industrial processing was compared among four biotechnologies: DNA barcoding, NGS, Sanger sequencing and qPCR. Results There was considerable variance in DNA quality and quantity among the samples, which could be interpreted along a gradient from raw materials with greater quantities (50–120 ng/μL) of DNA and longer DNA (400-500bp) sequences to extracts, which were characterized by lower quantities (0.1–10.0 ng/μL) and short fragments (50-150bp). Conclusions Targeted molecular diagnostic tests for species identity can be used in the NHP industry for raw and processed samples. Non-targeted tests or the use of NGS for any identity test needs considerable research and development and must be validated before it can be used in commercial operations as these methods are subject to considerable risk of false negative and positive results. Proper use of these tools can be used to ensure ingredient authenticity, and to avert adulteration, and contamination with plants that are a health concern. Lastly these tools can be used to prevent the exploitation of rare herbal species and the harvesting of native biodiversity for commercial purposes.

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“…Medicinal plants have been used in traditional medicine for centuries due to their therapeutic properties. Although they are mostly consumed as herbal infusions and tinctures, employment as ingredients of herbal and food supplements is increasing worldwide [1]. Consumers' awareness about healthy diets and their benefits is expanding the botanicals and herbal supplements market [1].…”

Section: Introductionmentioning

confidence: 99%

DNA-Based Herbal Teas’ Authentication: An ITS2 and psbA-trnH Multi-Marker DNA Metabarcoding Approach

Frigerio

Agostinetto

Mezzasalma

et al. 2021

Plants

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Medicinal plants have been widely used in traditional medicine due to their therapeutic properties. Although they are mostly used as herbal infusion and tincture, employment as ingredients of food supplements is increasing. However, fraud and adulteration are widespread issues. In our study, we aimed at evaluating DNA metabarcoding as a tool to identify product composition. In order to accomplish this, we analyzed fifteen commercial products with DNA metabarcoding, using two barcode regions: psbA-trnH and ITS2. Results showed that on average, 70% (44–100) of the declared ingredients have been identified. The ITS2 marker appears to identify more species (n = 60) than psbA-trnH (n = 35), with an ingredients’ identification rate of 52% versus 45%, respectively. Some species are identified only by one marker rather than the other. Additionally, in order to evaluate the quantitative ability of high-throughput sequencing (HTS) to compare the plant component to the corresponding assigned sequences, in the laboratory, we created six mock mixtures of plants starting both from biomass and gDNA. Our analysis also supports the application of DNA metabarcoding for a relative quantitative analysis. These results move towards the application of HTS analysis for studying the composition of herbal teas for medicinal plants’ traceability and quality control.

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Visualization of DNA in highly processed botanical materials

Cited by 15 publications

References 28 publications

Monitoring and traceability of genetically modified soya bean event GTS 40-3-2 during soya bean protein concentrate and isolate preparation

Monitoring and traceability of genetically modified soya bean event GTS 40-3-2 during soya bean protein concentrate and isolate preparation

Exploring DNA quantity and quality from raw materials to botanical extracts

DNA-Based Herbal Teas’ Authentication: An ITS2 and psbA-trnH Multi-Marker DNA Metabarcoding Approach

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