Visualization of Food Structure by Confocal Laser Scanning Microscopy (CLSM)

Dürrenberger, Markus; Handschin, Stephan; Conde‐Petit, Béatrice; Escher, F.

doi:10.1006/fstl.2000.0739

Cited by 154 publications

(57 citation statements)

References 15 publications

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“…It is a method, which can provide detailed morphology by visualizing clear fluorescent images of structures of a biosystem 26 . Images of thick sections of specimen can be obtained 4 . CSLM allows staining of specific compounds, and hence the distribution of these compounds through the kernel can be more apparent then when other microscopy techniques are used.…”

Section: Introductionmentioning

confidence: 99%

Microstructure of Buckwheat and Barley During Malting Observed by Confocal Scanning Laser Microscopy and Scanning Electron Microscopy

Wijngaard

Renzetti

Arendt

2007

Journal of the Institute of Brewing

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The structures of buckwheat and barley were observed by Scanning Electron Microscopy (SEM) and Confocal Scanning Laser Microscopy (CSLM) throughout the malting process. SEM and CSLM have different merits when studying grains and seeds. SEM can be used to study the detailed structure (high magnifications can be achieved) and CSLM to study the overall change in structure during processing. SEM proved that buckwheat starch is degraded by both pitting and surface erosion. A concentric sphere structure was visible when buckwheat starch granules were partly broken down. With CSLM, the organization of protein and starch of buckwheat and barley could be examined independently in projections and images visualizing the 3D structure. CSLM proved that buckwheat starch is surrounded by a protein matrix. Buckwheat protein was structured and surrounded starch granules, while barley protein was present in an amorphous mass through the endosperm. The differences in overall structure between unmalted and malted seeds were evident, as the starch and protein in both the barley and buckwheat were degraded. CSLM may be used in the future to couple processing characteristics to the structure of grains and seeds.

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Section: Introductionmentioning

confidence: 99%

Microstructure of Buckwheat and Barley During Malting Observed by Confocal Scanning Laser Microscopy and Scanning Electron Microscopy

Wijngaard

Renzetti

Arendt

2007

Journal of the Institute of Brewing

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“…It is a method that can provide detailed morphology by visualizing clear images of structures of a bio system 20 . Optical sections through thick sections of a specimen can be obtained 4 . CSLM allows staining of specific compounds and hence the distribution of these compounds through the kernel can be more apparent compared to other microscopy techniques.…”

Section: Introductionmentioning

confidence: 99%

Proso millet (Panicum miliaceumL.): An Evaluation of the Microstructural Changes in the Endosperm during the Malting Process by Using Scanning-Electron and Confocal Laser Microscopy

Zarnkow

Mauch

Back

et al. 2007

Journal of the Institute of Brewing

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Scanning electron microscopy (SEM) and confocal scanning laser microscopy (CLSM) were used to investigate the microstructural changes in the endosperm during the malting process. SEM was a suitable tool to characterise the microstructural constitution of starch granules in proso millet and the changes occurring during malting. An early visible degradation (after 24 h) of starch granules located in the floury endosperm, which is close to the embryo, could be observed. Due to this degradation, using confocal scanning laser microscopy, a less dense packaging of this part of the endosperm was observed. Degradation of starch granules in the vitreous endosperm was obvious at a distinct later stage of malting (78 h) and a preferred attack of small granules (<2.5 µm) was detected. Changes in protein structure could not be detected by either SEM or CLSM.

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“…Recently, confocal laser scanning microscopy (CLSM) could be regarded as a potential tool for understanding the effect of processing and the infl uence on bio-macromolecules. A microstructural change in instant noodles during production has been studied using CLSM to monitor microstructural differences (Dürrenberger, et al, 2001;Lalana, 2001;Sangpring, et al, 2015).…”

Section: Microscopic Imagining Of Noodlesmentioning

confidence: 99%

Interactions of Bio-macromolecules During Processing of Instant Asian Noodles: A Review

Shrestha¹,

Prasad²,

Anal³

2016

J Food Sci Technol Nepal

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Visualization of Food Structure by Confocal Laser Scanning Microscopy (CLSM)

Cited by 154 publications

References 15 publications

Microstructure of Buckwheat and Barley During Malting Observed by Confocal Scanning Laser Microscopy and Scanning Electron Microscopy

Microstructure of Buckwheat and Barley During Malting Observed by Confocal Scanning Laser Microscopy and Scanning Electron Microscopy

Proso millet (Panicum miliaceumL.): An Evaluation of the Microstructural Changes in the Endosperm during the Malting Process by Using Scanning-Electron and Confocal Laser Microscopy

Interactions of Bio-macromolecules During Processing of Instant Asian Noodles: A Review

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