Visualization of galanin binding sites in the rat central nervous system

Physiology, Signaling, and Pharmacology of Galanin Peptides and Receptors: Three Decades of Emerging Diversity

“…Galanin receptors were initially mapped using radioligand binding autoradiography, first in the rat Melander et al, 1988) and then the primate brain (Kohler et al, 1989a,b;Kohler and Chan-Palay, 1990). However, in the mid-1990s the first galanin receptor gene, GAL 1 , was cloned from a human melanoma cell line (Habert-Ortoli et al, 1994).…”

“…125 I]galanin binding sites Melander et al, 1988), the distribution of GAL 1 , GAL 2 , and GAL 3 mRNAs was reported in rat and mouse brain (and spinal cord), using both in situ hybridization and RT-PCR (see below). Although there are literature reports of the distribution of GAL 1-3 proteins using polyclonal antisera and immunohistochemistry, the validity of these data has been questioned and must be considered only putative mappings that require independent validation (see section IV.C).…”

Upregulation of Galanin Binding Sites and GalR1 mRNA Levels in the Mouse Locus Coeruleus Following Chronic Morphine Treatments and Precipitated Morphine Withdrawal

“…The neuropeptide galanin (Melander et al 1986b) and galanin binding sites (Melander et al 1986a;Skofitsch et al 1986) are present in most norepinephrine containing neurons of the locus coeruleus (LC), a brain area that plays a role in opiate dependence and the expression of somatic withdrawal symptoms (Aghajanian 1978;Nestler 1992). In vitro studies have shown that activation of galanin receptors inhibits the spontaneous firing of LC neurons (Pieribone et al 1995;Seutin et al 1989;Sevcik et al 1993).…”

Aqueduct Occlusion Does Not Impair Feeding Induced by Either Third or Fourth Ventricle Galanin Injection

“…The hindbrain site responsible for stimulation of feeding by galanin may be the AP/NTS region, which contains galaninergic terminals, cell bodies and receptors (20,21,28,29) and contributes to body-weight regulation and food intake (3,7,8,11,15). Recent results indicate a specific involvement of AP/NTS galanin terminals in the control of food intake by fatty-acid metabolism.…”

“…Galanin terminals or cell bodies are located in several brain sites important for food intake, including the paraventricular nucleus of the hypothalamus (PVN), the area postrema/nucleus of the solitary tract (AP/NTS) and the central nucleus of the amygdala (20,21,28,29,32). Injection of exogenous galanin into these sites increases feeding (9,10,(16)(17)(18)30,33).…”