Visualization of Golgi Apparatus in Methacrylate Embedded Conifer Embryo Tissue Using the Monoclonal Antibody Jim 84

Abstract

Methacrylate embedding followed by resin removal has been used for the first time to visualize a membrane-associated antigen at the tissue level. Monoclonal antibody JIM 84 was used to stain the Golgi apparatus of gymnosperm (conifer) embryos by light microscope immunocytochemistry. Specificity of labelling was confirmed by electron microscope immunocytochemistry using LR-white resin. GA staining was evident in all stages of white spruce somatic embryo development from immature to mature. Some regions of the somatic embryos (e.g. root cap/suspensor region) stained more vigorously than other regions (hypocotyl/cotyledon end). GA also stained in roots of Monterey pine and Douglas fir. Unlike the situation in most angiosperms, JIM 84 antigen appears to be absent from the conifer plasma membrane. However, it appears to be present in representatives of both major classes of higher plants.

Structural requirements for Arabidopsisβ1,2‐xylosyltransferase activity and targeting to the Golgi

“…Furthermore, the immunolocalization of this fusion protein gave a similar pattern as observed after immunostaining for plant Le a structures. These glycoepitopes are acknowledged plant Golgi markers (Evans et al, 1997;Essl et al, 1999;Fitchette et al, 1999;Saint-Jore et al, 2002). Moreover and as previously described for other Golgi-specific labelings in plant cells, BFA induces a redistribution of XylT36::GFP from Golgi membranes to the ER (Driouich et al, 1993;Boevink et al, 1998;Pagny et al, 2000;SaintJore et al, 2002).…”

Overexpression of Arabidopsis AGD7 Causes Relocation of Golgi-Localized Proteins to the Endoplasmic Reticulum and Inhibits Protein Trafficking in Plant Cells

“…Anti-g-COP recognizes g-COP, which is present in COPI vesicles that localize to the Golgi complex (Pimpl et al, 2000), and the monoclonal antibody JIM84 recognizes Lewis a-containing N-glycans in the trans-Golgi (Evans et al, 1997;Fitchette et al, 1999). HA:AGD7 produced a punctate staining pattern in cryosections of transgenic plant tissues (Fig.…”

‘Airborne’ contact dermatitis due to Leica immersion oil

“…In neuroanatomy, silicone resins are used for the fixation of brain tissue, and polyester resins allow sections to be obtained that differentiate gray from white matter 18 . Methacrylate embedding followed by resin removal facilitates visualization of the Golgi apparatus or the cytoskeleton 19 , 20 . In nephrology, renal biopsies are studied by immunoelectron microscopy after embedding in epoxy resin 21 …”