Visualization of in vitro deep blood vessels using principal component analysis based laser speckle imaging

Arias-Cruz, Jose Angel; Chiu, Roger; Peregrina-Barreto, Hayde; Ramos-Garcı́a, R.; Spezzia-Mazzocco, Teresita; Ramírez-San-Juan, J. C.

doi:10.1364/boe.10.002020

Cited by 18 publications

(7 citation statements)

References 21 publications

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“…Due to its popularity, LSCI instrument development is an active area of research with microfluidic studies commonly used to characterize performance in controlled flow environments. Typically, studies designed to mimic blood flow involve the use of a flow channel embedded in a polydimethylsiloxane (PDMS) substrate, 14 glass capillary tubing, 22 or clear plastic tubing 23 . More complex microfluidic networks have also been fabricated in glass, plastic, or epoxy substrates to represent superficial heterogenous vasculature 24 .…”

Section: Introductionmentioning

confidence: 99%

“…More complex microfluidic networks have also been fabricated in glass, plastic, or epoxy substrates to represent superficial heterogenous vasculature 24 . A scattering solution 14 , 22 or whole blood 23 is then flowed through the channel at controlled rates and imaged using LSCI. For applications in rodent studies, 0.1 to 10 mm/s is a commonly selected range of flow speeds that corresponds with experimentally measured capillary speeds 25 .…”

Section: Introductionmentioning

confidence: 99%

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Using pressure-driven flow systems to evaluate laser speckle contrast imaging

et al. 2023

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Significance: Microfluidic flow phantom studies are commonly used for characterizing the performance of laser speckle contrast imaging (LSCI) instruments. The selection of the flow control system is critical for the reliable generation of flow during testing. The majority of recent LSCI studies using microfluidics used syringe pumps for flow control.Aim: We quantified the uncertainty in flow generation for a syringe pump and a pressure-regulated flow system. We then assessed the performance of both LSCI and multi-exposure speckle imaging (MESI) using the pressure-regulated flow system across a range of flow speeds. Approach:The syringe pump and pressure-regulated flow systems were evaluated during stepped flow profile experiments in a microfluidic device using an inline flow sensor. The uncertainty associated with each flow system was calculated and used to determine the reliability for instrument testing. The pressure-regulated flow system was then used to characterize the relative performance of LSCI and MESI during stepped flow profile experiments while using the inline flow sensor as reference.Results: The pressure-regulated flow system produced much more stable and reproducible flow outputs compared to the syringe pump. The expanded uncertainty for the syringe pump was 8 to 20× higher than that of the pressure-regulated flow system across the tested flow speeds. Using the pressure-regulated flow system, MESI outperformed single-exposure LSCI at all flow speeds and closely mirrored the flow sensor measurements, with average errors of 4.6% AE 2.6% and 15.7% AE 4.6%, respectively.Conclusions: Pressure-regulated flow systems should be used instead of syringe pumps when assessing the performance of flow measurement techniques with microfluidic studies. MESI offers more accurate relative flow measurements than traditional LSCI across a wide range of flow speeds.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Using pressure-driven flow systems to evaluate laser speckle contrast imaging

et al. 2023

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“…Typically, studies designed to mimic blood flow involve the use of a flow channel embedded in a polydimethylsiloxane (PDMS) substrate, 14 glass capillary tubing, 22 or clear plastic tubing. 23 More complex microfluidic networks have also been fabricated in glass, plastic, or epoxy substrates to represent superficial heterogenous vasculature.…”

Section: Introductionmentioning

confidence: 99%

“…Due to its popularity, LSCI instrument development is an active area of research with microfluidic studies commonly used to characterize performance in controlled flow environments. Typically, studies designed to mimic blood flow involve the use of a flow channel embedded in a polydimethylsiloxane (PDMS) substrate, 14 glass capillary tubing, 22 or clear plastic tubing. 23 More complex microfluidic networks have also been fabricated in glass, plastic, or epoxy substrates to represent superficial heterogenous vasculature.…”

Section: Introductionmentioning

confidence: 99%

“…23 More complex microfluidic networks have also been fabricated in glass, plastic, or epoxy substrates to represent superficial heterogenous vasculature. 24 A scattering solution 14, 22 or whole blood 23 is then flowed through the channel at controlled rates and imaged using LSCI. For applications in rodent studies, 0.1 – 10 mm/s is a commonly selected range of flow speeds that corresponds with experimentally measured capillary speeds.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Using pressure-driven flow systems to evaluate laser speckle contrast imaging

Sullender

Santorelli

Richards

et al. 2022

Preprint

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Significance: Microfluidic flow phantom studies are commonly used for characterizing the performance of laser speckle contrast imaging (LSCI) instruments. The selection of the flow control system is critical for the reliable generation of flow during testing. The majority of recent LSCI studies using microfluidics used syringe pumps for flow control. Aim: We quantified the uncertainty in flow generation for a syringe pump and a pressure-regulated flow system. We then assessed the performance of both LSCI and multi-exposure speckle imaging (MESI) using the pressure-regulated flow system across a range of flow speeds. Approach: The syringe pump and pressure-regulated flow systems were evaluated during stepped flow profile experiments in a microfluidic device using an inline flow sensor. The uncertainty associated with each flow system was calculated and used to determine the reliability for instrument testing. The pressure-regulated flow system was then used to characterize the relative performance of LSCI and MESI during stepped flow profile experiments while using the inline flow sensor as reference. Results: The pressure-regulated flow system produced much more stable and reproducible flow outputs compared to the syringe pump. The expanded uncertainty for the syringe pump was 8-20× higher than that of the pressure-regulated flow system across the tested flow speeds. Using the pressure-regulated flow system, MESI outperformed single-exposure LSCI at all flow speeds and closely mirrored the flow sensor measurements, with average errors of 4.6±2.6% and 15.7±4.6%, respectively. Conclusions: Pressure-regulated flow systems should be used instead of syringe pumps when assessing the performance of flow measurement techniques with microfluidic studies. MESI offers more accurate relative flow measurements than traditional LSCI across a wide range of flow speeds.

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