Visualization of internal in situ cell structure by atomic force microscopy

Segura-Valdez, María L; Agredano-Moreno, Lourdes Teresa; Zamora-Cura, Alma; Lara‐Martínez, Reyna; Jiménez‐García, Luis Felipe

doi:10.1007/s00418-018-1721-6

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…In all these processes, the inherent underlying mechanism at the physical level may be viewed as a form of vibration. Sophisticated tools, including atomic force microscopy (AFM) [ 1 , 2 , 3 , 4 ], scanning tunneling microscopy (STM) [ 5 , 6 ], terahertz near-field microscopy (THz-NFM) [ 7 ], and hyperspectral imaging (HSI) [ 8 , 9 , 10 ], are now helping to offer a glimpse of vibrational patterning at the subcellular and cellular levels.…”

Section: An Ensemble Of Physical Energies Acting As the Control Softw...mentioning

confidence: 99%

Cell Responsiveness to Physical Energies: Paving the Way to Decipher a Morphogenetic Code

Tassinari¹,

Cavallini²,

Olivi³

et al. 2022

IJMS

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We discuss emerging views on the complexity of signals controlling the onset of biological shapes and functions, from the nanoarchitectonics arising from supramolecular interactions, to the cellular/multicellular tissue level, and up to the unfolding of complex anatomy. We highlight the fundamental role of physical forces in cellular decisions, stressing the intriguing similarities in early morphogenesis, tissue regeneration, and oncogenic drift. Compelling evidence is presented, showing that biological patterns are strongly embedded in the vibrational nature of the physical energies that permeate the entire universe. We describe biological dynamics as informational processes at which physics and chemistry converge, with nanomechanical motions, and electromagnetic waves, including light, forming an ensemble of vibrations, acting as a sort of control software for molecular patterning. Biomolecular recognition is approached within the establishment of coherent synchronizations among signaling players, whose physical nature can be equated to oscillators tending to the coherent synchronization of their vibrational modes. Cytoskeletal elements are now emerging as senders and receivers of physical signals, “shaping” biological identity from the cellular to the tissue/organ levels. We finally discuss the perspective of exploiting the diffusive features of physical energies to afford in situ stem/somatic cell reprogramming, and tissue regeneration, without stem cell transplantation.

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Section: An Ensemble Of Physical Energies Acting As the Control Softw...mentioning

confidence: 99%

Cell Responsiveness to Physical Energies: Paving the Way to Decipher a Morphogenetic Code

Tassinari¹,

Cavallini²,

Olivi³

et al. 2022

IJMS

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“…In addition to Young's modulus, the internal compound and products of vital activity of living cells are determining characteristics [16]. The ability to study these parameters, to obtain mechanical characteristics and topographic maps of living cells at the same time, will significantly expand the SICM toolkit.…”

Section: Introductionmentioning

confidence: 99%

Single-Cell Analysis with Silver-Coated Pipette by Combined SERS and SICM

Dubkov,

Overchenko,

Novikov

et al. 2023

Cells

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The study of individual cell processes that occur both on their surface and inside is highly interesting for the development of new medical drugs, cytology and cell technologies. This work presents an original technique for fabricating the silver-coated pipette and its use for the cell analysis by combination with surface-enhanced Raman spectroscopy (SERS) and scanning ion-conducting microscopy (SICM). Unlike the majority of other designs, the pipette opening in our case remains uncovered, which is important for SICM. SERS-active Ag nanoparticles on the pipette surface are formed by vacuum–thermal evaporation followed by annealing. An array of nanoparticles had a diameter on the order of 36 nm and spacing of 12 nm. A two-particle model based on Laplace equations is used to calculate a theoretical enhancement factor (EF). The surface morphology of the samples is investigated by scanning electron microscopy while SICM is used to reveal the surface topography, to evaluate Young’s modulus of living cells and to control an injection of the SERS-active pipettes into them. A Raman microscope–spectrometer was used to collect characteristic SERS spectra of cells and cell components. Local Raman spectra were obtained from the cytoplasm and nucleus of the same HEK-293 cancer cell. The EF of the SERS-active pipette was 7 × 105. As a result, we demonstrate utilizing the silver-coated pipette for both the SICM study and the molecular composition analysis of cytoplasm and the nucleus of living cells by SERS. The probe localization in cells is successfully achieved.

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“…As in the universe, in biological organisms, rhythmic oscillations and syn-chronization of oscillatory patterns are an essential requisite for recognition and connectedness. Sophisticated approaches, including atomic force microscopy (AFM)[1-4], scanning tunneling microscopy (STM)[5,6], terahertz field microscopy (TFM)[7], and hyperspectral imaging (HSI)[8-10] are now providing a dynamic picture of the cellular environment at a nanoscale level, showing that mobile elements of the cyto- and nucleo-skeleton are dancing with patterns that display features of coherence, short- and long-range signal propagation, networking, and memory. Tubulin dimers, and microtubules are now emerging as the constituents of a highly dynamic web, acting both as a source for the generation and the context for the interplay of physical energies[5,6].…”

Section: Introductionmentioning

confidence: 99%

Physical energies to the rescue of damaged tissues

Facchin

Canaider

Tassinari³

et al. 2019

WJSC

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Rhythmic oscillatory patterns sustain cellular dynamics, driving the concerted action of regulatory molecules, microtubules, and molecular motors. We describe cellular microtubules as oscillators capable of synchronization and swarming, generating mechanical and electric patterns that impact biomolecular recognition. We consider the biological relevance of seeing the inside of cells populated by a network of molecules that behave as bioelectronic circuits and chromophores. We discuss the novel perspectives disclosed by mechanobiology, bioelectromagnetism, and photobiomodulation, both in term of fundamental basic science and in light of the biomedical implication of using physical energies to govern (stem) cell fate. We focus on the feasibility of exploiting atomic force microscopy and hyperspectral imaging to detect signatures of nanomotions and electromagnetic radiation (light), respectively, generated by the stem cells across the specification of their multilineage repertoire. The chance is reported of using these signatures and the diffusive features of physical waves to direct specifically the differentiation program of stem cells in situ , where they already are resident in all the tissues of the human body. We discuss how this strategy may pave the way to a regenerative and precision medicine without the needs for (stem) cell or tissue transplantation. We describe a novel paradigm based upon boosting our inherent ability for self-healing.

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Visualization of internal in situ cell structure by atomic force microscopy

Cited by 5 publications

References 29 publications

Cell Responsiveness to Physical Energies: Paving the Way to Decipher a Morphogenetic Code

Cell Responsiveness to Physical Energies: Paving the Way to Decipher a Morphogenetic Code

Single-Cell Analysis with Silver-Coated Pipette by Combined SERS and SICM

Physical energies to the rescue of damaged tissues

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