Visualization of microtubules in living cells of transgenicArabidopsis thaliana

Ueda, Katsumi; Matsuyama, Takashi; Hashimoto, Takashi

doi:10.1007/bf01279267

Cited by 217 publications

(206 citation statements)

References 28 publications

Supporting

Mentioning

203

Contrasting

Order By: Relevance

“…No signal was observed in the leaves of 35S:AtSulA-GFP plants, and a very weak signal was detectable in their roots, whereas a very strong signal was detected in control plants expressing a tubulin-GFP fusion (Ueda et al, 1999) (Figure 7D). Thus, the overall accumulation of AtSulA-GFP in 35S:AtSulA-GFP plants is very low.…”

Section: The Variability Of the Phenotype Is Independent Of Transcripmentioning

confidence: 99%

An Arabidopsis Homolog of the Bacterial Cell Division Inhibitor SulA Is Involved in Plastid Division[W]

et al. 2004

View full text Add to dashboard Cite

Plastids have evolved from an endosymbiosis between a cyanobacterial symbiont and a eukaryotic host cell. Their division is mediated both by proteins of the host cell and conserved bacterial division proteins. Here, we identified a new component of the plastid division machinery, Arabidopsis thaliana SulA. Disruption of its cyanobacterial homolog (SSulA) in Synechocystis and overexpression of an AtSulA-green fluorescent protein fusion in Arabidopsis demonstrate that these genes are involved in cell and plastid division, respectively. Overexpression of AtSulA inhibits plastid division in planta but rescues plastid division defects caused by overexpression of AtFtsZ1-1 and AtFtsZ2-1, demonstrating that its role in plastid division may involve an interaction with AtFtsZ1-1 and AtFtsZ2-1.

show abstract

Section: The Variability Of the Phenotype Is Independent Of Transcripmentioning

confidence: 99%

An Arabidopsis Homolog of the Bacterial Cell Division Inhibitor SulA Is Involved in Plastid Division[W]

et al. 2004

View full text Add to dashboard Cite

show abstract

“…Arabidopsis thaliana ecotype Col-0 was used. Seeds of Arabidopsis plants expressing the green fluorescent protein-tubulin α6 (GFP-TUA6) marker were kindly provided by T. Hashimoto (Ueda et al 1999), and transgenic plants expressing the GFP-mTalin, were generated in the lab with a plasmid kindly provided by N. Chua (Kost et al 1998).…”

Section: Methodsmentioning

confidence: 99%

Herbicidal Activity of Monoterpenes Is Associated with Disruption of Microtubule Functionality and Membrane Integrity

et al. 2016

View full text Add to dashboard Cite

Aromatic plants and their volatile compounds affect seed germination and plant growth, and therefore hold potential for agriculture uses as plant growth regulators and bioherbicides. In the present study 17 major monoterpenes were selected, and their mechanisms of plant toxicity were elucidated using transgenic Arabidopsis thaliana at various growth stages. Microtubulin and the plant cell membrane were identified as the focal targets through which phytotoxicity and herbicidal activity acted. Variability in monoterpene mechanisms was observed. Limonene and (+ )-citronellal had strong antimicrotubule efficacy, whereas citral, geraniol, (−)-menthone, (+)-carvone, and (−)-citronellal demonstrated moderate antimicrotubule efficacy. Pulegone, (−)-carvone, carvacrol, nerol, geranic acid, (+)/(−)-citronellol, and citronellic acid lacked antimicrotubule capacity. An enantioselective disruption of microtubule assembly was recorded for (+)/(−)-citronellal and (+)/(−)-carvone. The (+) enatiomers were more potent than their (−) counterparts. Citral, limonene, carvacrol, and pulegone were also tested for phytotoxicity and herbicidal activity. Pulegone had no detectable effect on microtubules or membranes. Citral disrupted microtubules but did not cause membrane damage. Carvacrol lacked a detectable effect on microtubules but incited membrane leakage, and limonene disrupted microtubules and membrane leakage. Therefore, only limonene was herbicidal at the tested concentrations. In planta quantification of residues revealed that citral was biotransformed into nerol and geraniol, and limonene was converted into carvacrol, which could explain its dual capacity with respect to microtubules and membrane functionality. The results obtained are an important added value to commercial efforts in selecting appropriate aromatic plants to be sources of bioherbicidal compounds for sustainable weed management with a limited potential for herbicide resistance evolution in weed populations.

show abstract

“…In plants, specific targeting of GFP has allowed the visualization of individual subcellular structures such as endoplasmic reticulum (Haseloff et al, 1997), Golgi stacks (Boevink et al, 1998;Nebenfü hr et al, 1999), mitochondria (Kö hler et al, 1997b), nuclei (Grebenok et al, 1997), plastids (Kö hler et al, 1997a;Sidorov et al, 1999), plasma membrane (Bischoff et al, 2000), vacuoles (Di Sansebastiano et al, 1998;Mitsuhashi et al, 2000), cell wall (Scott et al, 1999), actin microfilaments (Kost et al, 1998), and microtubules (Marc et al, 1998;Ueda et al, 1999). GFPlabeled peroxisomes have been visualized by Cutler et al (2000) and Mano et al (1999).…”

Section: Peroxisome Labeling and Covisualization With Cytoskeletal Elmentioning

confidence: 99%

Simultaneous Visualization of Peroxisomes and Cytoskeletal Elements Reveals Actin and Not Microtubule-Based Peroxisome Motility in Plants,

2002

View full text Add to dashboard Cite

Peroxisomes were visualized in living plant cells using a yellow fluorescent protein tagged with a peroxisomal targeting signal consisting of the SKL motif. Simultaneous visualization of peroxisomes and microfilaments/microtubules was accomplished in onion (Allium cepa) epidermal cells transiently expressing the yellow fluorescent protein-peroxi construct, a green fluorescent protein-mTalin construct that labels filamentous-actin filaments, and a green fluorescent proteinmicrotubule-binding domain construct that labels microtubules. The covisualization of peroxisomes and cytoskeletal elements revealed that, contrary to the reports from animal cells, peroxisomes in plants appear to associate with actin filaments and not microtubules. That peroxisome movement is actin based was shown by pharmacological studies. For this analysis we used onion epidermal cells and various cell types of Arabidopsis including trichomes, root hairs, and root cortex cells exhibiting different modes of growth. In transient onion epidermis assay and in transgenic Arabidopsis plants, an interference with the actin cytoskeleton resulted in progressive loss of saltatory movement followed by the aggregation and a complete cessation of peroxisome motility within 30 min of drug application. Microtubule depolymerization or stabilization had no effect.

show abstract

Visualization of microtubules in living cells of transgenicArabidopsis thaliana

Cited by 217 publications

References 28 publications

An Arabidopsis Homolog of the Bacterial Cell Division Inhibitor SulA Is Involved in Plastid Division[W]

An Arabidopsis Homolog of the Bacterial Cell Division Inhibitor SulA Is Involved in Plastid Division[W]

Herbicidal Activity of Monoterpenes Is Associated with Disruption of Microtubule Functionality and Membrane Integrity

Simultaneous Visualization of Peroxisomes and Cytoskeletal Elements Reveals Actin and Not Microtubule-Based Peroxisome Motility in Plants,

Contact Info

Product

Resources

About