Visualization of nanoconstructions with DNA-Aptamers for targeted molecules binding on the surface of screen-printed electrodes

Лапин, И. Н.; Shabalina, Anastasiia V.; Svetlichnyi, V. A.; Kolovskaya, Olga S.

doi:10.1117/12.2304745

Cited by 1 publication

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“…In this work, to check whether the aptamer’s binding ability was not affected by the immobilization at the surface and to study the aptamer layer structure possible change after contact with the real samples (blood plasma), we suggested, for the first time, to apply the CLSM method [ 64 , 65 ].…”

Section: Resultsmentioning

confidence: 99%

Development of Electrochemical Aptasensor for Lung Cancer Diagnostics in Human Blood

Shabalina

Sharko

Glazyrin

et al. 2021

Sensors

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We describe the preparation and characterization of an aptamer-based electrochemical sensor to lung cancer tumor markers in human blood. The highly reproducible aptamer sensing layer with a high density (up to 70% coverage) on the gold electrode was made. Electrochemical methods and confocal laser scanning microscopy were used to study the stability of the aptamer layer structure and binding ability. A new blocking agent, a thiolated oligonucleotide with an unrelated sequence, was applied to fill the aptamer layer’s defects. Electrochemical aptasensor signal processing was enhanced using deep learning and computer simulation of the experimental data array. It was found that the combinations (coupled and tripled) of cyclic voltammogram features allowed for distinguishing between the samples from lung cancer patients and healthy candidates with a mean accuracy of 0.73. The capacitive component from the non-Faradic electrochemical impedance spectroscopy data indicated the tumor marker’s presence in a sample. These findings allowed for the creation of highly informative aptasensors for early lung cancer diagnostics.

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Section: Resultsmentioning

confidence: 99%