Visualization of Sorption Dynamics: Application of Confocal Laser Scanning Microscope Technique

Hasegawa, Takashi; Matsumoto, Kiyoichi; Matsui, T.

doi:10.5772/14551

Cited by 1 publication

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References 40 publications

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“…The concentration of HA in the gels was measured by confocal laser scanning microscopy (CLSM). CLSM measures the fluorescence intensity in optical sections of a sample 39 and has been used to follow mass transport phenomena including penetration of compounds into polymer matrices 40 and alginate gels. 41 After exposure to HA, the gels were placed on a microscopic slide and immediately analyzed on a Zeiss Axiovert 200 M microscope (Carl Zeiss, Germany) equipped with an argon laser set at 488 nm (stabilized for 30 min prior to measurements).…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Partitioning of Humic Acids between Aqueous Solution and Hydrogel: Concentration Profiling of Humic Acids in Hydrogel Phases

et al. 2014

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The partitioning of the natural polyelectrolyte humic acid (HA) from an aqueous dispersion into a model biomimetic gel (alginate) and a synthetic polyacrylamide gel (PAAm) is explored. In both gels, the spatial distribution of HA in the gel body, as measured by confocal laser scanning microscopy, is markedly nonhomogeneous. A striking feature is the enhanced accumulation of HA in a thin film of thickness ca. 15 μm at the surface of the gel body, resulting in average local concentrations that are, for PAAm and alginate respectively, a factor of 10 and 4 greater than that in the bulk solution. The time dependence of accumulation in the surface film is predominantly controlled by the diffusive supply of HA from the aqueous medium, with a time constant on the order of 10(3) s for both gels. The concentration of HA within the bulk gel body differs significantly from that in the bulk aqueous medium: substantially higher for PAAm but much lower for alginate. The results are significant for understanding the nature and rate of sink/source functioning at permeable phases in contact with aqueous media, e.g., biofilms and gel-like layers at biological interfaces or employed in chemical speciation sensors.

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Section: ■ Experimental Sectionmentioning

confidence: 99%