Visualization of the distribution of anthraquinone components from madder roots in rat kidneys by desorption electrospray ionization-time-of-flight mass spectrometry imaging

Ishii, Yuji; Nakamura, Kenji; Mitsumoto, Tatsuya; Takimoto, Norifumi; Namiki, Moeka; Takasu, Shinji; Ogawa, Kumiko

doi:10.1016/j.fct.2022.112851

Cited by 9 publications

(2 citation statements)

References 32 publications

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“…Though previous reports suggested alizarin could be used as an anticancer drug [4,5,29], up to now, no sufficient in vivo data were available to support this proposal. On the contrary, assays with rats indicated the carcinogenic potential of alizarin by showing greater distribution in the outer stripe of the outer medulla, increasing 8-OHdG levels and exerting tumor-promoting effects in the kidney [10,30,31]. Consistently, our data also indicated the carcinogenesis of alizarin, where the DO analysis for the DEGs induced by alizarin showed that "Cancer" was one of the most enriched categories (Figure 4c).…”

Section: Chemicals and Cell Culturesupporting

confidence: 80%

Alizarin, an Agonist of AHR Receptor, Enhances CYP1A1 Enzyme Activity and Induces Transcriptional Changes in Hepatoma Cells

Liang,

Bo,

Zhang

et al. 2023

Molecules

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The phytopigment alizarin was previously characterized as an anti-tumor drug owing to its antioxidant or antigenotoxic activities. However, the safety of alizarin is currently still under dispute. In this study, we explored the activity of alizarin in the AHR-CYP1A1 pathway and analyzed the transcriptional changes affected by alizarin using human hepatoma cell line HepG2-based assays. The results showed that alizarin decreased HepG2 cell viability in a dose-dependent manner, with IC50 values between 160.4 and 216.8 μM. Furthermore, alizarin significantly upregulated the expression of CYP1A1 and increased the ethoxyresorufin-O-deethylase activity. Alizarin also exhibited agonistic activity toward the AHR receptor in the XRE-mediated luciferase reporter gene assay, which was further confirmed via the molecular docking assay. In addition, the transcriptional analysis indicated that alizarin may act as a potential carcinogen through significantly enriching several items related to cancer in both DO and KEGG analysis. In brief, our findings indicated that alizarin shows agonistic activities to the AHR receptor through activating the AHR-CYP1A1 signaling pathway in HepG2 cells, which may lead to the risks for cancer developing.

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Section: Chemicals and Cell Culturesupporting

confidence: 80%

Alizarin, an Agonist of AHR Receptor, Enhances CYP1A1 Enzyme Activity and Induces Transcriptional Changes in Hepatoma Cells

Liang,

Bo,

Zhang

et al. 2023

Molecules

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show abstract

“…The ability to perform rapid tissue imaging has become one of the major applications in AIMS research, with DESI‐MS being the most popular technique for this purpose. The past 12 months have seen an abundance of studies harnessing DESI for imaging of biological tissues, particularly in murine models, such as imaging of alkaloids in rat brains, 134 visualizing the distribution of carcinogens in the kidneys of rats, 135 and imaging of mouse and rat brains with convolutional neural network‐based deep learning. 136 In addition, numerous modifications to the original DESI technique have emerged to improve mass spectral imaging capabilities.…”

Section: Applications Of Aimsmentioning

confidence: 99%

Applications of ambient ionization mass spectrometry in 2022: An annual review

Rankin‐Turner

Sears

Heaney

2023

Analytical Science Advances

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The development of ambient ionization mass spectrometry (AIMS) has transformed analytical science, providing the means of performing rapid analysis of samples in their native state, both in and out of the laboratory. The capacity to eliminate sample preparation and pre‐MS separation techniques, leading to true real‐time analysis, has led to AIMS naturally gaining a broad interest across the scientific community. Since the introduction of the first AIMS techniques in the mid‐2000s, the field has exploded with dozens of novel ion sources, an array of intriguing applications, and an evident growing interest across diverse areas of study. As the field continues to surge forward each year, ambient ionization techniques are increasingly becoming commonplace in laboratories around the world. This annual review provides an overview of AIMS techniques and applications throughout 2022, with a specific focus on some of the major fields of research, including forensic science, disease diagnostics, pharmaceuticals and food sciences. New techniques and methods are introduced, demonstrating the unwavering drive of the analytical community to further advance this exciting field and push the boundaries of what analytical chemistry can achieve.

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Site-specific genotoxicity of rubiadin: localization and histopathological changes in the kidneys of rats

Mitsumoto,

Ishii,

Takimoto

et al. 2023

Arch Toxicol

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Visualization of the distribution of anthraquinone components from madder roots in rat kidneys by desorption electrospray ionization-time-of-flight mass spectrometry imaging

Cited by 9 publications

References 32 publications

Alizarin, an Agonist of AHR Receptor, Enhances CYP1A1 Enzyme Activity and Induces Transcriptional Changes in Hepatoma Cells

Alizarin, an Agonist of AHR Receptor, Enhances CYP1A1 Enzyme Activity and Induces Transcriptional Changes in Hepatoma Cells

Applications of ambient ionization mass spectrometry in 2022: An annual review

Site-specific genotoxicity of rubiadin: localization and histopathological changes in the kidneys of rats

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