Visualization of the metaphase II meiotic spindle in living human oocytes using the Polscope enables the prediction of embryonic developmental competence after ICSI

Moon, Jeong-Hee; Hyun, Chang-Seop; Lee, Seok-Won; Son, Weon‐Young; Yoon, San-Hyun; Lim, Jin‐Ho

doi:10.1093/humrep/deg165

Cited by 150 publications

(134 citation statements)

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“…Our results support the observations of others that the presence of a birefringent spindle in human oocytes could predict a higher fertilization rate and a greater probability of embryo development (11,12). Thus, spindle visualization (spindle-based selection of oocytes) can help differentiate oocytes, and the presence of spindle can serve as an early indicator of oocyte viability (11,16,21).…”

Section: Discussionsupporting

confidence: 90%

“…Our results show that just prior to ICSI in almost one fourth of the oocytes the spindle is in the "dangerous zone" since it was found in the "route" of injection pipette at 3 or 9 o'clock. Our results, supporting others' observations, indicate that first polar body position does not always accurately predict spindle position (11,12,22).…”

Section: Discussionsupporting

confidence: 77%

“…Thus, spindle visualization (spindle-based selection of oocytes) can help differentiate oocytes, and the presence of spindle can serve as an early indicator of oocyte viability (11,16,21). However, the relative position of the spindle within the oocytes does not appear to influence the developmental potential of embryos (12).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that the presence of birefringent spindles predicts higher fertilization rates and that the position of the polar body is not an accurate predictor of spindle position in mammalian oocytes (8,(11)(12)(13). In this study we examined 1) whether spindles in living human oocytes could safely be imaged/examined by the polscope during clinical application of IVF/ICSI-ET and 2) the effect of detectability and location of meiotic spindle to fertilization of human oocytes with ICSI.…”

Section: Introductionmentioning

confidence: 99%

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Visualization and Examination of the Meiotic Spindle in Human Oocytes with Polscope

Konc

Kanyó

Cseh

2004

J Assist Reprod Genet

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Purpose : The effect of detectability/location of meiotic spindle to fertilization of human MII oocytes and outcome of clinical IVF/ICSI-ET cycles was studied. Methods : The location of spindle relative to polar body positioned to 6 o'clock was detected with polscope prior to ICSI in oocytes. Data of 83 IVF/ICSI-ET cycle, including pregnancy results, and a total of 1033 oocytes examined were analyzed retrospectively. Results : Polar body position does not always accurately predict spindle position. Similar fertilization was observed in the polscope detected and in the control group. The main number of oocytes and spindle positive oocytes collected was higher in the pregnant than in the nonpregnant group. Higher fertilization was found in that group where 2/3-d of the oocytes had detected spindle. The main number of oocytes and spindle positive oocytes obtained decreased with increasing age of patients. Conclusions : The presence of birefringent spindle predicts higher fertilization and development and greater probability of pregnancy.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Visualization and Examination of the Meiotic Spindle in Human Oocytes with Polscope

Konc

Kanyó

Cseh

2004

J Assist Reprod Genet

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“…Total time for intracytoplasmic sperm injection did not exceed 10 min. Oocytes that extruded a polar body after denudation were not included into the study in order not to introduce bias into groups as the incidence of poor fertilization/poor embryo development has been shown to be higher in latematured oocytes [17].…”

Section: Methodsmentioning

confidence: 99%

Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study

Ciray¹,

Aksoy²,

Göktaş³

et al. 2012

J Assist Reprod Genet

145

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Objective To compare the dynamics of early development between embryos cultured in single and sequential media. Design Randomized, comparative study. Setting Private IVF centre. Patients A total of 446 metaphase II oocytes from 51 couples who underwent oocyte retrieval procedure for intracytoplasmic sperm injection. Forty-nine resulted in embryo transfer. Intervention Oocytes were split between single and sequential media produced by the same manufacturer and cultured in a time-lapse incubator. Main outcome measures Morphokinetic parameters until the embryos reached the 5-cell stage (t5), utilization, clinical pregnancy and implantation rates. Results Embryos cultured in single media were advanced from the first mitosis cycle and reached 2-to 5-cell stages earlier. There was not any difference between the durations for cell cycle two (cc20t3-t2) and s2 (t4-t3). The utilization, clinical pregnancy and implantation rates did not differ between groups. The proportion of cryopreserved day6 embryos to two pronuclei oocytes was significantly higher in sequential than in single media. Conclusions Morphokinetics of embryo development vary between single and sequential culture media at least until the 5-cell stage. The overall clinical and embryological parameters remain similar regardless of the culture system.

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Novel Imaging Techniques to Assess Gametes and Preimplantation Embryos

Swain

2016

Human Reproduction

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Visualization of the metaphase II meiotic spindle in living human oocytes using the Polscope enables the prediction of embryonic developmental competence after ICSI

Abstract: the presence of a bi-refringent meiotic spindle in human oocytes by using the Polscope can predict a higher embryonic developmental competence. However, the relative position of the spindle within the oocyte doesn't appear to influence the developmental potential of embryos.

Cited by 150 publications

References 14 publications

Visualization and Examination of the Meiotic Spindle in Human Oocytes with Polscope

Visualization and Examination of the Meiotic Spindle in Human Oocytes with Polscope

Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study

Novel Imaging Techniques to Assess Gametes and Preimplantation Embryos

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