Visualizing Dipeptidyl Peptidase-IV with an Advanced Non-π-Conjugated Fluorescent Probe for Early Thyroid Disease Diagnosis

Fabrication of a Near-Infrared-Emissive Probe for Detecting Dipeptidyl Peptidase 4 in the Liver of Diabetic Mice and Clinical Serum

“…By virtue of NIR emission and stable optical and chemical properties, cyano isophorone fluorophore was selected as the probe skeleton and signal reporter (Scheme ). − Subsequently, a dipeptide glycylprolyl (Gly-Pro, consisting of proline and alanine), a known DPP4 responsive unit, − was modified on the amino group of dye skeleton to receive probe NEDP . The intrinsically nonfluorescent probe NEDP can be sensitively catalyzed and degraded by DPP4 to release the strongly fluorescent dye NEDD in vitro and in vivo , which brought a remarkable turn-on NIR fluorescence emission (673 nm).…”

“…With the development of fluorescence imaging technology, it has become one of the commonly used techniques in biological research, due to its inherent advantages including excellent selectivity and sensitivity, easy management, nondestructiveness, and high spatial resolution. − Among optical probes, especially near-infrared (NIR)-emissive probes received increased attention lately because of the deeper tissue penetration depth and the lower background interference. − Recently, some fluorescent probes to detect and monitor DPP4 in biological processes have been witnessed (Table S1). − However, few of them studied DPP4 fluctuation in liver and clinical serum during diabetes. Herein, a NIR-emissive fluorescent DPP4 probe ( NEDP ) was developed for specific detection and imaging of DPP4 in a biological process.…”