Visualizing the osmotic water permeability of a lipid bilayer under measured bilayer tension using a moving membrane method

Yano, Kazuhiro; Iwamoto, Masayuki; Koshiji, Takaaki; Oiki, Shigetoshi

doi:10.1016/j.memsci.2021.119231

Cited by 2 publications

(5 citation statements)

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“…Electrode setting and electrophysiological measurements: Electrical measurements were performed for evaluating membrane capacitance and currents through the membrane-incorporated gramicidin A channel [9] . AgCl ink (BAS, 011464, Tokyo) was painted at one end for the capillary's inner surface [1] . For the other open end of the capillary, KCl-bridged Ag/AgCl electrodes were inserted into the electrolyte solution.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…The membrane capacitance was measured by applying the ramp potential (±10 mV/ms). Given the specific membrane capacitance [1] , the membrane area was estimated.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…In the moving membrane method, the velocity of the moving membrane, vel m , is measured visually (Table 1, lower), from which P f is evaluated as [1]

where A cap is the capillary cross-sectional area (µm 2 ), v w is the partial molar volume of water (cm 3 /mol), vel m is the membrane velocity (µm/s), A mem is the membrane area (µm 2 ), and Δ c s is the osmotic gradient (mOsm/L) ( Table 1) . In the P f evaluation, correction of unstirred layer (UL) is a prerequisite, which is evaluated as follows.…”

Section: Data Descriptionmentioning

confidence: 99%

“…(1) such that J v / A mem is divided by v w and ΔOsm multiplied by 10 6 . These data are plotted in the reference [1] Fig. 3 B.…”

Section: Data Descriptionmentioning

confidence: 99%

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Geometrical and electrophysiological data of the moving membrane method for the osmotic water permeability of a lipid bilayer

et al. 2021

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Data of the osmotic water permeability of a lipid bilayer (diphytanoylphosphaticylcholin) in the presence of cholesterol (30 mole%) are shown under the simultaneous measurement of bilayer tension. Detailed methods and procedures for evaluating the water permeability using the moving membrane method (K. Yano, M. Iwamoto, T. Koshiji & S. Oiki: Visualizing the Osmotic Water Permeability of a Lipid Bilayer under Measured Bilayer Tension Using a Moving Membrane Method. Journal of Membrane Science, 627 (2021) 119231) are presented. The planar lipid bilayer is formed in a glass capillary, separating two aqueous compartments with different osmolarities, and osmotically-driven water flux is visualized as membrane movements along the capillary. The water permeability was evaluated under constant membrane area and tension after correcting for the unstirred layer effect. In these measurements, geometrical features, such as the edge of the planar lipid bilayer and the contact angle between bilayer and monolayer, were image-analyzed. The unstirred layer was evaluated electrophysiologically, in which gramicidin A channel was employed. In the presence of an osmotic gradient, the gramicidin channel generates the streaming potential, and the measured streaming potential data and the derived water-ion coupling ratio (water flux/ion flux) are shown. Detailed descriptions of the integrated method of the moving membrane allow researchers to reproduce the experiment and give opportunities to examine water permeability of various types of membranes, including those containing aquaporins. The present data of osmotic water permeability are compared with the previously published data, while they neglected the bilayer tension.

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Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…The membrane capacitance was measured by applying the ramp potential (±10 mV/ms). Given the specific membrane capacitance [1] , the membrane area was estimated.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…In the moving membrane method, the velocity of the moving membrane, vel m , is measured visually (Table 1, lower), from which P f is evaluated as [1]

Section: Data Descriptionmentioning

confidence: 99%

“…(1) such that J v / A mem is divided by v w and ΔOsm multiplied by 10 6 . These data are plotted in the reference [1] Fig. 3 B.…”

Section: Data Descriptionmentioning

confidence: 99%

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Geometrical and electrophysiological data of the moving membrane method for the osmotic water permeability of a lipid bilayer

et al. 2021

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“…In biological membranes, which are rich in unsaturated lipids at the sn -2 position and are generally in a fluid liquid crystalline phase under physiological conditions, cholesterol incorporation increases their physical stability, − decreases the lateral diffusion rate, ,− and reduces the permeation of water and hydrophilic solutes across the bilayer, − although the level of hydration in the interfacial region is increased to the depth of the acyl carbonyl groups. − For amphiphilic organic molecules and peptides, the effects of cholesterol on permeability and partitioning vary with membrane composition and the nature of the partitioning molecule. − …”

Section: Introductionmentioning

confidence: 99%

The Chemical Reactivity of Membrane Lipids

Duché,

Sanderson

2024

Chem. Rev.

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It is well-known that aqueous dispersions of phospholipids spontaneously assemble into bilayer structures. These structures have numerous applications across chemistry and materials science and form the fundamental structural unit of the biological membrane. The particular environment of the lipid bilayer, with a water-poor low dielectric core surrounded by a more polar and better hydrated interfacial region, gives the membrane particular biophysical and physicochemical properties and presents a unique environment for chemical reactions to occur. Many different types of molecule spanning a range of sizes, from dissolved gases through small organics to proteins, are able to interact with membranes and promote chemical changes to lipids that subsequently affect the physicochemical properties of the bilayer. This Review describes the chemical reactivity exhibited by lipids in their membrane form, with an emphasis on conditions where the lipids are well hydrated in the form of bilayers. Key topics include the following: lytic reactions of glyceryl esters, including hydrolysis, aminolysis, and transesterification; oxidation reactions of alkenes in unsaturated fatty acids and sterols, including autoxidation and oxidation by singlet oxygen; reactivity of headgroups, particularly with reactive carbonyl species; and E/Z isomerization of alkenes. The consequences of reactivity for biological activity and biophysical properties are also discussed. CONTENTS

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Visualizing the osmotic water permeability of a lipid bilayer under measured bilayer tension using a moving membrane method

Cited by 2 publications

References 54 publications

Geometrical and electrophysiological data of the moving membrane method for the osmotic water permeability of a lipid bilayer

Geometrical and electrophysiological data of the moving membrane method for the osmotic water permeability of a lipid bilayer

The Chemical Reactivity of Membrane Lipids

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