VIT-CMJ2: Endophyte of Agaricus bisporus in Production of Bioactive Compounds

Gautam, Chandan Kumar; Madhav, Mukund; Sinha, Ayush; Osborne, W. Jabez

doi:10.15171/ijb.1287

Cited by 9 publications

(5 citation statements)

References 17 publications

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“…Mantovani et al 17 reported that only after two-year cryopreservation it is possible to verify the effect of long-term preservation on mycelial viability maintenance. Moreover, the long-term cryopreservation could affect not only the mycelial cell survival but also other microorganisms inside the mycelia such as endosymbionts, similar to endophytes, 31 which could interfere with mycelial growth.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation at −75 °C of Agaricus subrufescens on wheat grains with sucrose

Júnior

Lopes

Cordeiro

et al. 2018

Brazilian Journal of Microbiology

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Agaricus subrufescens is a basidiomycete which is studied because of its medicinal and gastronomic importance; however, less attention has been paid to its preservation. This study aimed to evaluate the effect of sucrose addition to substrate and cryotube on the viability of Agaricus subrufescens cryopreserved at −20 °C and at −75 °C for one and two years. Zero, 10% or 20% sucrose was added to potato dextrose agar or wheat grain. The mycelia were cryopreserved in the absence of cryoprotectant or with sucrose solutions at 15%, 30% or 45%. After one or two years at −75 °C or at −20 °C, mycelia were thawed and evaluated about viability, initial time of growth, colony diameter and genomic stability. Cryopreservation at −20 °C is not effective to keep mycelial viability of this fungus. Cryopreservation at −75 °C is effective when sucrose is used in substrates and/or cryotubes. Without sucrose, cryopreservation at −75 °C is effective only when wheat grains are used. Physiological characteristic as mycelial colony diameter is negatively affected when potato dextrose agar is used and unaffected when wheat grain is used after two-year cryopreservation at −75 °C. The fungus genome does not show alteration after two-year cryopreservation at −75 °C.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation at −75 °C of Agaricus subrufescens on wheat grains with sucrose

Júnior

Lopes

Cordeiro

et al. 2018

Brazilian Journal of Microbiology

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“…By the neighbor-joining tree technique, the phylogenetic tree was built. The consensus sequences were deposited in GenBank (Gautam et al, 2016(Gautam et al, ). 10.3389/fmicb.2024 Frontiers in Microbiology 04 frontiersin.org…”

Section: Molecular Genomic Recognition Of Endophytic Fungusmentioning

confidence: 99%

“…Turbo mass software (5.4.2 version) was used for the spectrum analysis. The configurations of the chemical were compared to the mass spectral outlines in the NIST collection (2008) (Gautam et al, 2016).…”

Section: Gc-ms Analysis Of Pts8 Fungal Crude Extractmentioning

confidence: 99%

Xenomyrothecium tongaense PTS8: a rare endophyte of Polianthes tuberosa with salient antagonism against multidrug-resistant pathogens

Sundar,

Arunachalam

2024

Front. Microbiol.

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IntroductionEndophytes refer to microorganisms residing within the endosphere of plants, particularly perennials, without inflicting noticeable injury or inducing obvious morphological variations to their host plant or host organism. Endophytic fungi, although often overlooked microorganisms, have garnered interest due to their significant biological diversity and ability to produce novel pharmacological substances.MethodsIn this study, fourteen endophytic fungi retrieved were from the stem of the perennial plant Polianthes tuberosa of the Asparagaceae family. These fungal crude metabolites were tested for antagonistic susceptibility to Multi-Drug Resistant (MDR) pathogens using agar well diffusion, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC) assays. The chequerboard test was used to assess the synergistic impact of active extract.Results and discussionIn early antibacterial screening using the Agar plug diffusion test, three of fourteen endophytes demonstrated antagonism against Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin-resistant Enterococcus (VRE). Three isolates were grown in liquid medium and their secondary metabolites were recovered using various organic solvents. Eight extracts from three endophytic fungi displayed antagonism against one or more human pathogens with diameters ranging from 11 to 24 mm. The highest antagonistic effect was obtained in ethyl acetate extract for PTS8 isolate against two MRSA (ATCC 43300, 700699) with 20 ± 0.27 and 22 ± 0.47 mm zones of inhibition, respectively, among different solvent extracts. The extract had MICs of 3.12 ± 0.05 and 1.56 ± 0.05 μg/mL, and MBCs of 50 ± 0.01 and 12.5 ± 0.04 μg/mL, respectively. Antagonism against VRE was 18 ± 0.23 mm Zone of Inhibition (ZOI) with MIC and MBC of 6.25 ± 0.25 and 25 ± 0.01 μg/mL. When ethyl acetate extract was coupled with antibiotics, the chequerboard assay demonstrated a synergistic impact against MDR bacteria. In an antioxidant test, it had an inhibitory impact of 87 ± 0.5% and 88.5 ± 0.5% in 2,2-Diphenyl-1-Picrylhydrazyl and reducing power assay, respectively, at 150 μg/mL concentration. PTS8 was identified as a Xenomyrothecium tongaense strain by 18S rRNA internal transcribed spacer (ITS) sequencing. To our insight, it is the foremost study to demonstrate the presence of an X. tongaense endophyte in the stem of P. tuberosa and the first report to study the antibacterial efficacy of X. tongaense which might serve as a powerful antibacterial source against antibiotic-resistant human infections.

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“…Endophytes were isolated according to the method described by Gautam et al (2016) with slight variations. To isolate the endophytic bacteria, the surface of the mushroom was sterilized with 4% sodium hypochlorite solution, 70% ethyl alcohol, and sterile double-distilled water (ddH 2 O).…”

Section: Isolation Of Endophytic Bacteriamentioning

confidence: 99%

Study of growth-improving and sporophore-inducing endobacteria isolated from Pleurotus pulmonarius

Paul,

Roy,

Singh

et al. 2023

World J Microbiol Biotechnol

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Several Pleurotusspecies (oyster mushrooms) are commercially cultivated in India owing to the favorable tropical agro-climatic conditions. However, there are only a few studies on the microbiome of mushrooms, especially oyster mushrooms. The aim of this study was to assess the effect of endophytic bacteria on mycelial growth, spawning, sporophore development, and proximate composition of P. pulmonarius. We isolated several bacterial strains from the sporophores of P. pulmonarius and assessed the in vitro production of indole acetic acid, ammonia, and siderophores. The selected bacteria were individually supplemented with spawn, substrate, or both for sporophore production. Three of 130 isolates were selected as mycelial growth-promoting bacteria in both solid and submerged fermentation. These bacterial isolates were identi ed through Gram staining, biochemical characterization, and 16S rRNA sequencing. Isolate PP showed 99.24% similarity with Priestia para exa, whereas isolates PJ1 and PJ2 showed 99.78% and 99.65% similarities, respectively, with Rossellomorea maris avi. The bacterial supplementation with spawn, substrate, or both, increased the biological e ciency (BE) and nutrient content of the mushrooms. The bacterial supplementation with substrate augmented BE by 64.84%, 13.73%, and 27.13% using PJ2, PP, and PJ1, respectively; under similar conditions of spawn supplementation, BE wasincreased by 15.24%, 47.03%, 48.10%, respectively. Overall, the supplementation of endophytic bacteria to improve oyster mushroom cultivation may open a new avenue for sustainable agricultural practices in the mushroom industry.

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VIT-CMJ2: Endophyte of Agaricus bisporus in Production of Bioactive Compounds

Cited by 9 publications

References 17 publications

Cryopreservation at −75 °C of Agaricus subrufescens on wheat grains with sucrose

Cryopreservation at −75 °C of Agaricus subrufescens on wheat grains with sucrose

Xenomyrothecium tongaense PTS8: a rare endophyte of Polianthes tuberosa with salient antagonism against multidrug-resistant pathogens

Study of growth-improving and sporophore-inducing endobacteria isolated from Pleurotus pulmonarius

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