Vital fluorescent dyes for study of silicifying organisms

Abstract

The mini-review describes application of vital fluorescent dyes to study silica biosynthesis in diatom algae and siliceous sponges. Mechanisms of these processes are not clear and study of growing siliceous constructions in living organisms is complicated with silica transparency and presence of mature elements (siliceous valves of diatoms and spicules of sponges). Specific fluorescence dyes stain growing siliceous elements of skeletons and also primary siliceous particles which are formed during silicon capture from the environment. Vital fluorescent dyes based on 7-nitro-2,1,3-benzoxadiazole, pyridyl oxazole and coumarin fluorophores are described as well as examples of application of these dyes to study diatom algae and siliceous sponges.

Testing the use of the silica deposition fluorescent probe PDMPO to estimate in situ growth rates of diatoms

“…The advantage of the later is to provide further information on phasing of division as well as the potential variability in growth rates within a species population. Furthermore, new development of vital stains, with different excitation and emission spectra, higher quantum yields could help overcome these caveats in the future (Annenkov et al 2018).…”