Vital Microscopy of Bone Remodelling in Rabbit Ear Chambers

Suomann, Einar

doi:10.3109/ort.1975.46.suppl-160.01

Cited by 16 publications

(23 citation statements)

References 33 publications

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“…These drugs inhibit disordered osteoclast activity, retard woven-fibered bone tissue formation, and impair fracture healing [1,2,23,34,37,52,54,62,63]. COX inhibitors are, however, very efficient drugs in the treatment of postoperative inflammation and pain in orthopaedic trauma and surgery.…”

Section: Introductionmentioning

confidence: 99%

Parecoxib and Indomethacin Delay Early Fracture Healing: A Study in Rats

Dimmen

Nordsletten

Madsen

2009

Clinical Orthopaedics &Amp; Related Research

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Nonsteroidal antiinflammatory drugs (NSAIDs) are used to reduce inflammatory response and pain. These drugs have been reported to impair bone metabolism. Parecoxib, a specific COX-2 inhibitor, exerts an inhibitory effect on the mineralization of fracture callus after a tibial fracture in rats. Decreased bone mineral density (BMD) at a fracture site may indicate impairment of early healing, casting doubt on the safety of using COX-2 inhibitors during the early treatment of diaphyseal fractures. Fortytwo female Wistar rats were randomly allocated to three groups. They were given parecoxib, indomethacin, or saline intraperitoneally for 7 days after being subjected to a closed tibial fracture stabilized with an intramedullary nail. Two and 3 weeks after surgery, the bone density at the fracture site was measured using dual energy xray absorptiometry (DEXA). Three weeks after the operation the rats were euthanized and the healing fractures were mechanically tested in three-point cantilever bending.Parecoxib decreased BMD at the fracture site for 3 weeks after fracture, indomethacin for 2 weeks. Both parecoxib and indomethacin reduced the ultimate bending moment and the bending stiffness of the healing fractures after 3 weeks. These results suggest COX inhibitors should be avoided in the early phase after fractures.

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Section: Introductionmentioning

confidence: 99%

Parecoxib and Indomethacin Delay Early Fracture Healing: A Study in Rats

Dimmen

Nordsletten

Madsen

2009

Clinical Orthopaedics &Amp; Related Research

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“…bone wax and fat grafts. Free fat grafts have given encouraging results in a few patients (Langenskiold 1967 and1975). Good results were also obtained in an experimental study in rabbits, but in about one-fifth of the animals the fat grafts apparently failed to correct the angular deformity (Osterman 1972).…”

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confidence: 78%

“…Previous studies have shown that both heterotopic and orthotopic bone formation can be inhibited by indomethacin (Sudmann 1975, R0 et al 1976, Sudmann & Hagen 1976, Almhsbakk & R~ysland 1977, Sudmann & Bang 1979. In the present study we wanted to test whether indomethacin could prevent the recurrence of partial closure of an epiphyseal plate in adolescent rabbits, permitting gradual correction of angular deformities by growth.…”

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confidence: 99%

“…Previous studies have shown that indomethacin treatment results in a non-specific inhibition of the osteoblastic activity triggered by postfracture and / or postoperative inflammation (Sudmann 1975, Almdsbakk & Raysland 1977, Sudrnann et al 1979). These studies suggest that if the bone bridge between the epiphysis and the metaphysis is operatively removed, administration of indomethacin can inhibit the osteoblastic activity that causes its reformation.…”

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confidence: 99%

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Inhibition of Partial Closure of Epiphyseal Plate in Rabbits by Indomethacin

Sudmann

Husby

Bang

1982

Acta Orthopaedica Scandinavica

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“…In one case, they were able to follow the formation and the later resorption of the same bone patch. Sudmann (1975) developed a rabbit ear chamber made of pure titanium, with a well of 100 microns to contain the bone graft. His optically superior chambers allowed him to make vital microscopic studies of bone growth over periods of 6 months.…”

Section: Introductionmentioning

confidence: 99%

Vital confocal microscopy in bone

et al. 1995

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Summary:We wished to exploit confocal microscopy for high spatial and temporal resolution vital microscopy in bone. To this end, we evolved implants with glass windows supported in titanium, which were placed in the medial proximal tibial plateau of the rabbit, and special small, self-focussing objectives (dry 10/0.25, water immersion 20/0.45, and oil immersion 45/0.65 and 120/1.0) which mated and matched to the conical window entrance section of the metal components. At intervals of up to 21 months after implant healing, these lenses were used to study live tissue using two genera of confocal microscope: multiple aperture disc, tandem scanning, microscopes for observation in reflection, and video rate confocal laser scanning microscopes for recording, mainly in the fluorescence mode. The latter allowed the study of a variety of intravenously administered substances, including fluorescein, fluorescein-dextrans, fluorescent microspheres, acridine orange, DASPMI, calcein, and tetracycline. We were able to remove blood, stain cells with fluorescent markers, and replace them into the circulation. Calcein and tetracycline bind to the mineral front in bone: this labelling was studied in progress. We observed that both substances partition and remain for long periods (at least days) in adipocytes. Further characterisation of the system used both confocal fluorescence and scanning electron microscopy methods in the study of retrieved implants. These studies showed that the subimplant cortical bone remodelled to a less compact structure with a rich microvasculature extremely close to bone. The points of attachment of bone to glass were found to involve coarse fibres, with the matrix containing large numbers of large cells: some of this tissue was cartilage and some immature bone. An amorphous, mineralised matrix was in immediate contact with glass. The results provide further confirmation of the general utility of high-scan speed confocal methodology in physiology.

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Vital Microscopy of Bone Remodelling in Rabbit Ear Chambers

Cited by 16 publications

References 33 publications

Parecoxib and Indomethacin Delay Early Fracture Healing: A Study in Rats

Parecoxib and Indomethacin Delay Early Fracture Healing: A Study in Rats

Inhibition of Partial Closure of Epiphyseal Plate in Rabbits by Indomethacin

Vital confocal microscopy in bone

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