Vital NETosis vs. suicidal NETosis during normal pregnancy and preeclampsia

Guillotin, Florence; Fortier, Mathieu; Portes, Marie; Dematteï, Christophe; Mousty, E.; Cochery-Nouvellon, Éva; Mercier, Éric; Chéa, Mathias; Letouzey, V.; Gris, Jean-Christophe; Bouvier, Sylvie

doi:10.3389/fcell.2022.1099038

Cited by 15 publications

(7 citation statements)

References 47 publications

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“…Although researches have proved that the formation of NETs improved the body's defense mechanism, NETs are believed to be capable of causing the body to break the pro-and anti-inflammation balance. 77,78 Apoptosis is cell shrinkage accompanied by DNA rupture and nucleus condensation. The whole cell forms apoptotic bodies through blebbing and other methods.…”

Section: The Formation Of Nets: Vital Netosis and Suicidal Netosismentioning

confidence: 99%

Targeting Neutrophil Extracellular Traps in Gouty Arthritis: Insights into Pathogenesis and Therapeutic Potential

Li,

Wu,

et al. 2024

JIR

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Gouty arthritis (GA) is an immune-mediated disorder characterized by severe inflammation due to the deposition of monosodium urate (MSU) crystals in the joints. The pathophysiological mechanisms of GA are not yet fully understood, and therefore, the identification of effective therapeutic targets is of paramount importance. Neutrophil extracellular traps (NETs), an intricate structure of DNA scaffold, encompassing myeloperoxidase, histones, and elastases -have gained significant attention as a prospective therapeutic target for gouty arthritis, due to their innate antimicrobial and immunomodulatory properties. Hence, exploring the therapeutic potential of NETs in gouty arthritis remains an enticing avenue for further investigation. During the process of gouty arthritis, the formation of NETs triggers the release of inflammatory cytokines, thereby contributing to the inflammatory response, while MSU crystals and cytokines are sequestered and degraded by the aggregation of NETs. Here, we provide a concise summary of the inflammatory processes underlying the initiation and resolution of gouty arthritis mediated by NETs. Furthermore, this review presents an overview of the current pharmacological approaches for treating gouty arthritis and summarizes the potential of natural and synthetic product-based inhibitors that target NET formation as novel therapeutic options, alongside elucidating the intrinsic challenges of these inhibitors in NETs research. Lastly, the limitations of HL-60 cell as a suitable substitute of neutrophils in NETs research are summarized and discussed. Series of recommendations are provided, strategically oriented towards guiding future investigations to effectively address these concerns. These findings will contribute to an enhanced comprehension of the interplay between NETs and GA, facilitating the proposition of innovative therapeutic strategies and novel approaches for the management of GA.

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Section: The Formation Of Nets: Vital Netosis and Suicidal Netosismentioning

confidence: 99%

Targeting Neutrophil Extracellular Traps in Gouty Arthritis: Insights into Pathogenesis and Therapeutic Potential

Li,

Wu,

et al. 2024

JIR

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“…Another form of NETosis is “vital” or NADPH oxidase complex‐independent signaling 24 . The neutrophils remain alive following “vital” NETosis, without NADPH oxidase complex.…”

Section: Two Key Forms Of Netosismentioning

confidence: 99%

“…Another form of NETosis is "vital" or NADPH oxidase complexindependent signaling. 24 The neutrophils remain alive following "vital" NETosis, without NADPH oxidase complex. "Vital" NETosis is stimulated through active platelets, microorganisms, or complements.…”

Section: "Vital" Netosismentioning

confidence: 99%

NETosis: Sculpting tumor metastasis and immunotherapy

Hu,

Wang,

Liu

2023

Immunological Reviews

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SummaryThe process of neutrophil extracellular traps (NETs) formation, called NETosis, is a peculiar death modality of neutrophils, which was first observed as an immune response against bacterial infection. However, recent work has revealed the unique biology of NETosis in facilitating tumor metastatic process. Neutrophil extracellular traps released by the tumor microenvironment (TME) shield tumor cells from cytotoxic immunity, leading to impaired tumor clearance. Besides, tumor cells tapped by NETs enable to travel through vessels and subsequently seed distant organs. Targeted ablation of NETosis has been proven to be beneficial in potentiating the efficacy of cancer immunotherapy in the metastatic settings. This review outlines the impact of NETosis at almost all stages of tumor metastasis. Furthermore, understanding the multifaceted interplay between NETosis and the TME components is crucial for supporting the rational development of highly effective combination immunotherapeutic strategies with anti‐NETosis for patients with metastatic disease.

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“…NETosis, an inflammatory cell death mode of neutrophils, manifests through the release of Neutrophil Extracellular Traps (NETs) upon activation (Fang et al 2022 ). These NETs, comprising depolymerized chromatin and intracellular granular proteins, function as a potent means for neutrophils to trap and eliminate pathogens (Guillotin et al 2022 ). NETosis, distinct from apoptosis and cell necrosis, is induced by various stimuli, including bacteria, fungi, viruses, activated platelets, and chemokines.…”

Section: Introductionmentioning

confidence: 99%

Prognostic impact and immunotherapeutic implications of NETosis-related prognostic model in clear cell renal cell carcinoma

Mao,

Huang,

Xue

et al. 2024

J Cancer Res Clin Oncol

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Background The ramifications of necroptosis on the prognostication of clear cell renal cell carcinoma (ccRCC) remain inadequately expounded. Methods A prognostic model delineating the facets of necroptosis in ccRCC was constructed, employing a compendium of algorithms. External validation was effectuated using the E-MTAB-1980 dataset. The exploration of immune infiltration scores was undertaken through the exploitation of multiple algorithms. Single-cell RNA sequencing data were procured from the GSE171306 dataset. Real-time quantitative PCR (RT-qPCR) was engaged to scrutinize the differential expression of SLC25A37 across cancer and paracancer tissues, as well as diverse cell lines. Assessments of proliferative and metastatic alterations in 769-P and 786-O cells were accomplished through Cell Counting Kit-8 (CCK8) and wound healing assays. Results The necroptosis-related signature (NRS) emerges as a discerning metric, delineating patients’ immune attributes, tumor mutation burden, immunotherapy response, and drug susceptibility. Single-cell RNA sequencing analysis unveils the marked enrichment of SLC25A37 in tumor cells. Concurrently, RT-qPCR discloses the overexpression of SLC25A37 in both ccRCC tissues and cell lines. SLC25A37 knockdown mitigates the proliferative and metastatic propensities of 769-P and 786-O cells, as evidenced by CCK8 and wound healing assays. Conclusion The NRS assumes a pivotal role in ascertaining the prognosis, tumor mutation burden, immunotherapy response, drug susceptibility, and immune cell infiltration features of ccRCC patients. SLC25A37 emerges as a putative player in immunosuppressive microenvironments, thereby providing a prospective avenue for the design of innovative immunotherapeutic targets for ccRCC.

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Vital NETosis vs. suicidal NETosis during normal pregnancy and preeclampsia

Cited by 15 publications

References 47 publications

Targeting Neutrophil Extracellular Traps in Gouty Arthritis: Insights into Pathogenesis and Therapeutic Potential

Targeting Neutrophil Extracellular Traps in Gouty Arthritis: Insights into Pathogenesis and Therapeutic Potential

NETosis: Sculpting tumor metastasis and immunotherapy

Prognostic impact and immunotherapeutic implications of NETosis-related prognostic model in clear cell renal cell carcinoma

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