Vital role for the J-domain protein Mdj1 in asexual development, multiple stress tolerance, and virulence of Beauveria bassiana

Wang, Jie; Ying, Sheng‐Hua; Hu, Yue; Feng, Ming‐Guang

doi:10.1007/s00253-016-7757-4

Cited by 25 publications

(20 citation statements)

References 42 publications

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“…Pil1A and Pil1B (tag loci: BBA_00276 and BBA_09711 respectively), two eisosome proteins playing opposite roles in autophagic control, were required for the fungal virulence due to blocked secretion of cuticle-degrading Pr1 proteases and inhibition of dimorphic transition in absence of each or both (Zhang et al, 2017). Mdj1 (tag locus: BBA_06930), another Hsp40 family member, also made a remarked contribution to the fungal virulence as well as conidiation capacity (Wang et al, 2017b). Aside from these hyperacetylated proteins, many more Kac proteins modified in Drpd3 were involved in cell rescue, defence and virulence (Table 1) and could likely contribute to the fungal virulence through cell response to host immunity although most of them have not been characterized in B. bassiana.…”

Section: Discussionmentioning

confidence: 99%

Essential role of Rpd3‐dependent lysine modification in the growth, development and virulence of Beauveria bassiana

Cai

Wang

Shao

et al. 2018

Environmental Microbiology

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Rpd3 is a class I histone deacetylase that reverses lysine acetylation thus influencing cellular processes and functions. However, its role in fungal insect pathogens has not been explored yet. Here we show that Rpd3-dependent lysine modification and gene expression orchestrate growth, conidiation and virulence in Beauveria bassiana. Deletion of Rpd3 resulted in severe growth defects on various carbon/nitrogen sources, 97% reduction in conidiation capacity and drastic attenuation in virulence. These phenotypes concurred with differential expression of 1479 proteins and hyperacetylation or hypoacetylation of 2227 lysine residues on 1134 proteins. Many of these proteins fell into carbon/nitrogen metabolism and cell rescue/defence/virulence, indicating vital roles of Rpd3-dependent protein expression and lysine modification in the fungal growth and virulence. Intriguingly, lysine residues of four core histones (H2A, H2B, H3 and H4) and many histone acetyltransferases were also hyper- or hypoacetylated in Δrpd3, suggesting direct and indirect roles for Rpd3 in genome-wide lysine modification. However, crucial development activators were transcriptionally repressed and not found in either proteome or acetylome. Single/double-site-directed H3K9/K14 mutations for hyper/hypoacetylation exerted significant impacts on conidiation and dimorphic transition crucial for fungal virulence. Altogether, Rpd3 mediates growth, asexual development and virulence through transcriptional/translational regulation and posttranslational lysine modification in B. bassiana.

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Section: Discussionmentioning

confidence: 99%

Essential role of Rpd3‐dependent lysine modification in the growth, development and virulence of Beauveria bassiana

Cai

Wang

Shao

et al. 2018

Environmental Microbiology

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“…Both Mas5 and Mdj1, the members of Hsp40 in B . bassiana , have been shown to be indispensable for the environmental adaptation and virulence [19, 20]. …”

Section: Discussionmentioning

confidence: 99%

“…Both Mas5 and Mdj1, the members of Hsp40 in B . bassiana , play a very important role in environmental adaptation and host infection [19, 20]. The deletion of mannitol-1-phosphate dehydrogenase (MPD) reduces the content of mannitol in B .…”

Section: Introductionmentioning

confidence: 99%

De novo transcriptome sequencing of Isaria cateniannulata and comparative analysis of gene expression in response to heat and cold stresses

Ding-feng

et al. 2017

PLoS ONE

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Isaria cateniannulata is a very important and virulent entomopathogenic fungus that infects many insect pest species. Although I. cateniannulata is commonly exposed to extreme environmental temperature conditions, little is known about its molecular response mechanism to temperature stress. Here, we sequenced and de novo assembled the transcriptome of I. cateniannulata in response to high and low temperature stresses using Illumina RNA-Seq technology. Our assembly encompassed 17,514 unigenes (mean length = 1,197 bp), in which 11,445 unigenes (65.34%) showed significant similarities to known sequences in NCBI non-redundant protein sequences (Nr) database. Using digital gene expression analysis, 4,483 differentially expressed genes (DEGs) were identified after heat treatment, including 2,905 up-regulated genes and 1,578 down-regulated genes. Under cold stress, 1,927 DEGs were identified, including 1,245 up-regulated genes and 682 down-regulated genes. The expression patterns of 18 randomly selected candidate DEGs resulting from quantitative real-time PCR (qRT-PCR) were consistent with their transcriptome analysis results. Although DEGs were involved in many pathways, we focused on the genes that were involved in endocytosis: In heat stress, the pathway of clathrin-dependent endocytosis (CDE) was active; however at low temperature stresses, the pathway of clathrin-independent endocytosis (CIE) was active. Besides, four categories of DEGs acting as temperature sensors were observed, including cell-wall-major-components-metabolism-related (CWMCMR) genes, heat shock protein (Hsp) genes, intracellular-compatible-solutes-metabolism-related (ICSMR) genes and glutathione S-transferase (GST). These results enhance our understanding of the molecular mechanisms of I. cateniannulata in response to temperature stresses and provide a valuable resource for the future investigations.

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“…The same strategy for the deletion of mas5 or mdj1 and associated backbone plasmids ( Wang et al, 2016 , 2017 ) were used to delete hsf1 , sfl1 , and skn7 (Gene ID: 19887609, 19887228, and 19884300, respectively) from the wild-type strain B. bassiana ARSEF2860 (designated WT herein). Briefly, the 5′ and 3′ coding/flanking fragments of each gene were amplified from the WT via PCR with paired primers (Supplementary Table S1 ) under the action of La Taq DNA polymerase (Promega, Madison, MI, United States) and inserted into p0380-bar.…”

Section: Methodsmentioning

confidence: 99%

“…The genomic database of B. bassiana ( Xiao et al, 2012 ) has Hsf1, Sfl1, and Skn7 orthologs and 28 HSPs, which fall into the small HSP, Hsp40, Hsp60, Hsp70, Hsp90, and Hsp100 families with each containing 1–15 members. However, either three HSF or most HSP genes remain functionally unexplored because only two hsp40 genes, i.e., mas5 and mdj1 , have been characterized in B. bassiana ( Wang et al, 2016 , 2017 ). It is unclear whether Hsf1, Sfl1, and Skn7 activate different families of HSP genes in an independent or cooperative manner.…”

Section: Introductionmentioning

confidence: 99%

Roles of Three HSF Domain-Containing Proteins in Mediating Heat-Shock Protein Genes and Sustaining Asexual Cycle, Stress Tolerance, and Virulence in Beauveria bassiana

Zhou

Ying

et al. 2018

Front. Microbiol.

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Heat-shock transcription factors (HSFs) with a HSF domain are regulators of fungal heat-shock protein (HSP) genes and many others vectoring heat-shock elements, to which the domain binds in response to heat shock and other stress cues. The fungal insect pathogen Beauveria bassiana harbors three HSF domain-containing orthologous to Hsf1, Sfl1, and Skn7 in many fungi. Here, we show that the three proteins are interrelated at transcription level, play overlapping or opposite roles in activating different families of 28 HSP genes and mediate differential expression of some genes required for asexual developmental and intracellular Na+ homeostasis. Expression levels of skn7 and sfl1 largely increased in Δhsf1, which is evidently lethal in some other fungi. Hsf1 was distinct from Sfl1 and Skn7 in activating most HSP genes under normal and heat-shocked conditions. Sfl1 and Skn7 played overlapping roles in activating more than half of the HSP genes under heat shock. Each protein also activated a few HSP genes not targeted by two others under certain conditions. Deletion of sfl1 resulted in most severe growth defects on rich medium and several minimal media at optimal 25°C while such growth defects were less severe in Δhsf1 and minor in Δskn7. Conidiation level was lowered by 76% in Δskn7, 62% in Δsfl1, and 39% in Δhsf1. These deletion mutants also showed differential changes in cell wall integrity, antioxidant activity, virulence and cellular tolerance to osmotic salt, heat shock, and UV-B irradiation. These results provide a global insight into vital roles of Hsf1, Sfl1, and Skn7 in B. bassiana adaptation to environment and host.

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Vital role for the J-domain protein Mdj1 in asexual development, multiple stress tolerance, and virulence of Beauveria bassiana

Cited by 25 publications

References 42 publications

Essential role of Rpd3‐dependent lysine modification in the growth, development and virulence of Beauveria bassiana

Essential role of Rpd3‐dependent lysine modification in the growth, development and virulence of Beauveria bassiana

De novo transcriptome sequencing of Isaria cateniannulata and comparative analysis of gene expression in response to heat and cold stresses

Roles of Three HSF Domain-Containing Proteins in Mediating Heat-Shock Protein Genes and Sustaining Asexual Cycle, Stress Tolerance, and Virulence in Beauveria bassiana

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