Vitale Reaktionen und Zeitschätzungen

Berg, S.

doi:10.1007/978-3-642-66006-1_10

Cited by 14 publications

(14 citation statements)

References 31 publications

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“…The extractable iron started to increase in the 2-day-old hematomas. This finding is in accordance with the observations that the first Berlin Nut positive macrophages can be seen in 24-48-h-old hematomas [1]. Also the activity of heine oxygenase, which is the rate-limiting enzyme in hemoglobin degradation, has been shown to be increased in 2-day-old hematomas [11].…”

Section: Discussionsupporting

confidence: 86%

“…Pigments containing iron have been demonstrated in 24-48-h-old hematomas although a positive Berlin blue reaction is observed more regularly in hematomas 4-8 days old [1]. Hamdy et al 1957 [2] tried to determine "the easily split iron" in cattle tissue bruises aged from 15 min to 9 days by incubating the bruised tissue in 0.4% HC1 for 24h at 37°C and then determining the iron as the ferrous compound of o-phenanthroline.…”

Section: Introductionmentioning

confidence: 98%

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Extractable iron in experimental hematomas

Laiho

1987

Z Rechtsmed

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The extractable iron and total iron were determined in experimental, subcutaneous hematomas (5 ml blood) of rats after different periods of vital time. The extractable iron was determined from homogenate extract obtained after 24-h incubation with concentrated HCl diluted 1:3 and protein precipitation. For the determination of total iron, the specimens were wet-ashed. The same iron determinations were also made for the corresponding control specimens of the subcutaneous tissue. Extractable iron started to rise over the control values in 2-day-old hematomas, being about 5 times higher after 3 days, about 15 times higher after 7 days, and about 20 times higher after 14 days. The ratio of extractable iron and total iron expressed as percentages was about 1% in 1-h and in 1-day-old hematomas, about 20% in 3-day-old hematomas, about 50% in 7-day-old hematomas, and about 65% in 14-day-old or older hematomas. The effect of autolysis for 3 days at room temperature was studied on 1-h-old hematomas. Extractable iron values were slightly higher in the autolyzed specimens, but the difference was statistically insignificant as compared to specimens taken immediately after death.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 98%

Extractable iron in experimental hematomas

Laiho

1987

Z Rechtsmed

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“…In some cases with advanced individual age we noticed a somewhat reduced staining intensity for this cell adhesion protein, but relevant differences, which have to be considered for a forensically applicable wound age estimation, could not be found. Berg [3] reported that the individual age influences, in particular, later changes in wound healing, but is of less importance for the beginning of reparative changes. Furthermore, the kind of lesion (stab/surgical wounds versus lacerations) also seems to have less influence on the earliest appearance of tenascin (and probably of other reparative changes) since tissue regeneration starts in particular from undamaged skin.…”

Section: Discussionmentioning

confidence: 99%

Localization of tenascin in human skin wounds ?An immunohistochemical study

et al. 1993

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“…In general erythrocyte extravasation and cellular reaction with infiltration of inflammatory cells is assumed to be a sign of vitality [4,27]. Since, however, bleeding can be induced after death, this notion should be interpreted with care.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical localization of fibronectin as a tool for the age determination of human skin wounds

et al. 1992

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Summary:We analyzed the distribution of fibronectin in routinely embedded tissue specimens from 53 skin wounds and 6 postmortem wounds. In postmortem wounds a faint but focal positive staining was exclusively found at the margin of the specimens which dit not extend into the adjacent stroma. Vital wounds were classified into 3 groups. The first comprising lesions with wound ages ranging from a few seconds to 30min, the second comprising those with wound ages upt to 3 weeks, and the third group with lesions more than 3 weeks old. Ten out of 17 lesions with a wound age up to 30 rain showed a clear positive reaction within the wound area. Three specimens in this group were completely negative, while in 4 additional cases the result was not significantly different from postmortem lesions. These 7 Cases were characterized by acute death with extremely short survival times (only seconds). In wounds up to 3 weeks old fibronectin formed a distinct network containing an increasing number of inflammatory cells corresponding to the wound age. In 2 cases with a survival time of 17 days and in all wounds older than 3 weeks fibronectin was restricted to the surface of fibroblasts and to parallel arranged fibers in the granulation tissue without any network structures. We present evidence that fibronectin is a useful marker for vital wounds with a survival time of more than a few minutes. Fibronectin appears before neutrophilic granulocytes migrate into the wound area. Since a faint positive fibronectin staining is seen in postmortem lesions and bleedings, we propose that only those wounds which show strong positive fibronectin staining also extending into the adjacent stroma should be regarded as vital.

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Vitale Reaktionen und Zeitschätzungen

Cited by 14 publications

References 31 publications

Extractable iron in experimental hematomas

Extractable iron in experimental hematomas

Localization of tenascin in human skin wounds ?An immunohistochemical study

Immunohistochemical localization of fibronectin as a tool for the age determination of human skin wounds

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