Vitamin A-dependent fucose-glycopeptide from rat tracheal epithelium

Bonanni, F; Luca, Luigi De

doi:10.1016/0304-4165(74)90282-7

Cited by 22 publications

(7 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On a subcellular level, vitamin-A is known to stimulate the synthesis of glycoprotein and RNA (7,13,29) and to bind to plasma proteins (28) and certain cytosol fractions (4,44). No direct effect on the cell membrane has been demonstrated.…”

Section: The Vitamin-a Model Systemmentioning

confidence: 99%

Vitamin-A-induced mucous metaplasia. An in vitro system for modulating tight and gap junction differentiation.

Elias¹,

Friend²

1976

The Journal of Cell Biology

205

View full text Add to dashboard Cite

Stratified squamous epithelia from 14-day chick embryo shank skin contain rare tight-junctional strands and only small gap junctions. Exposure of this tissue to retinoic acid (vitamin-A) (20 U/ml) in organ culture, however, induces mucous metaplasia, accompanied by tight-junction formation and gap-junction growth; untreated specimens continue to keratinize. To investigate sequential stages of junctional assembly and growth, we examined thin sections and freeze-fracture replicas at daily intervals for 3 days. During the metaplastic process, tight junctions assemble in midepidermal and upper regions, beginning on day 1 and becoming maximal on day 3. Two tight-junctional patterns could be tentatively identified as contributing to the emergence of fully formed zonulae occludentes: (a) the formation of individual ridges along the margins of gap junctions; (b) de novo generation of continuous ramifying strands by fusion of short strand segments and linear particulate aggregates near cellular apices. Gap junction enlargement, already maximal at day 1, occurs primarily three to four cell layers deep. Growth appears to occur by annexation of islands of 20-40 8.5-nm particles into larger lattices of islands separated by particle-free aisles. Eventually, a single gap junction may occupy much of the exposed membrane face in freeze-fractured tissue, but during apical migration of the cells such junctions disappear. The vitamin-A chick-skin system is presented as a responsive model for the controlled study of junction assembly.The tight junction (zonula occludens) is a barrier to large molecule exchange between the lumina and intercellular spaces of epithelial tissues. Forming a continuous belt around the apices of polarized epithelia, it is visualized in thin section as a series of pentalaminar fusions between adjacent cells, and in freeze-fractured specimens as a network of fibrils or ridges and complementary furrows on the A and B faces, respectively (reviewed in references 23, 36, and 48). Whereas the distribution of this junction (16,19,43), its architecture (9,19,25,30), permeability properties (10,19,20), breakdown (48), and presumed mode of growth (37) have been described, a relatively simple laboratory model for inducing tight junctions in an epithelium normally deficient in such cellular components has been heretofore unavailable.The gap junction (nexus) is a highly ordered plasma membrane specialization considered essen-

show abstract

Section: The Vitamin-a Model Systemmentioning

confidence: 99%

Vitamin-A-induced mucous metaplasia. An in vitro system for modulating tight and gap junction differentiation.

Elias¹,

Friend²

1976

The Journal of Cell Biology

205

View full text Add to dashboard Cite

show abstract

“…This caused a reduced content of retinol in the liver but the sertim concentration was maintained. A reduction of discrete glycoproteios is reported during vitamin A deficiency from studies on small intestine (25), cornea (26), trachea (27), and serum (28). At a reduced intake of vitamin A the transporting protein, retinol binding protein (RBP), is affected.…”

Section: Discussionmentioning

confidence: 99%

“…When the biologic activity of two .specific glycoproteins, BAGP and SP, were compared, a decrease was found in the activity of BAGP but not of SP. A reduction of discrete glycoproteios is reported during vitamin A deficiency from studies on small intestine (25), cornea (26), trachea (27), and serum (28). DE LDCA et al (25) found that the number of goblet cells in the small intestine decreased in vitamin A deficient rats.…”

Section: Discussionmentioning

confidence: 99%

Effect of a moderate vitamin A deficiency on saliva secretion rate and some salivary glycoproteins in adult rat

Johansson

Lumikari

Ericson

1989

European J Oral Sciences

View full text Add to dashboard Cite

– The study presents the effect of a low vitamin A intake on saliva secretion rate and some salivary glycoproteins in the adult rat. Sixteen rats in the experimental group were fed a vitamin A deficient diet (0.11 μg retinol/g diet) and 14 rats in the control group a diet with adequate content of vitamin A (4.74 μg/g diet). At the end of the experimental period of 10 wk, whole saliva, blood, and liver samples were collected. No difference in the serum content of retinol was seen between the two groups. The liver values were significantly lower for the rats in the experimental group compared to the values in the control group. No difference was seen between the two groups in saliva secretion rate, salivary peroxidase activity, or the concentrations of total protein and markers for total glycoprotein secretion. However, the activity of a bacteria agglutinating glycoprotein, BAGP, was significantly reduced in the rats fed the vitamin A deficient diet.

show abstract

“…Subsequently, Bonanni et al (10,11) showed that when relatively stable macromolecular products such as glycopeptides were the object for study, such products could be isolated from tracheal epithelial cells that were enzymatically dissociated from the underlying supportive structures by incubation with hyaluronidase. Even smaller quantities of tissue than those used above apparently have been sufficient for studies of gelelectrophoretic analyses of LDH isozymes in the bronchi of rats exposed intratracheally to benzo(a)pyrene (BP).…”

Section: Methods For Biochemical Study Of Tracheobronchial Epitheliummentioning

confidence: 99%

“…On the macromolecular level, it was hypothesized (9,24) (7,10,11,76,77) as the result of a decreased capacity to manufacture mucus glycoproteins with specific sugar moieties. Further support for a role for vitamin A in glucoprotein synthesis came from work by Clark and Marchok (76,77 (35,36,(38)(39)(40)78) as an assay for retinoid potency.…”

Section: Rna Metabolism and Inhibitors Of Rna Synthesismentioning

confidence: 99%

Biochemical Studies of the Tracheobronchial Epithelium

Mass¹,

Kaufman²

1984

Environmental Health Perspectives

View full text Add to dashboard Cite

Tracheobronchial epithelium has been a focus of intense investigation in the field of chemical carcinogenesis. We have reviewed some biochemical investigations that have evolved through linkage with carcinogenesis research. These areas of investigation have included kinetics of carcinogen metabolism, identification of carcinogen metabolites, levels of carcinogen binding to DNA, and analysis of carcinogen-DNA adducts. Such studies appear to have provided a reasonable explanation for the susceptibilities of the respiratory tracts of rats and hamsters to carcinogenesis by benzo(a)pyrene. Coinciding with the attempts to understand the initiation of carcinogenesis in the respiratory tract has also been a major thrust aimed at effecting its prevention both in humans and in animal models for human bronchogenic carcinoma. These studies have concerned the effects of derivatives of vitamin A (retinoids) and their influence on normal cell biology and biochemistry of this tissue. Recent investigations have included the effects of retinoid deficiency on the synthesis of RNA and the identification of RNA species associated with this biological state, and also have included the effects of retinoids on the synthesis of mucus-related glycoproteins.Tracheal organ cultures from retinoid-deficient hamsters have been used successfully to indicate the potency of synthetic retinoids by monitoring the reversal of squamous metaplasia. Techniques applied to this tissue have also served to elucidate features of the metabolism of retinoic acid using high pressure liquid chromatography. In brief, formidable strides have been made in biochemistry specific to this important target tissue, despite the inability to acquire tracheobronchial epithelium in large quantities.

show abstract

Vitamin A-dependent fucose-glycopeptide from rat tracheal epithelium

Cited by 22 publications

References 8 publications

Vitamin-A-induced mucous metaplasia. An in vitro system for modulating tight and gap junction differentiation.

Vitamin-A-induced mucous metaplasia. An in vitro system for modulating tight and gap junction differentiation.

Effect of a moderate vitamin A deficiency on saliva secretion rate and some salivary glycoproteins in adult rat

Biochemical Studies of the Tracheobronchial Epithelium

Contact Info

Product

Resources

About