Vitamin A-Induced Synthesis of Alkaline Phosphatase

Riley, Patrick A.; Spearman, R. I. C.

doi:10.1126/science.160.3831.1006

Cited by 22 publications

(9 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been suggested that vitamin A regulates alkaline phosphatase activity in some cell types [5]. In the same study, similar results have been found in cultured prostatic cells of normal and malignant origin and found that retinoic acid causes the rapid RNA and protein synthesis-dependent induction of alkaline phosphatase.…”

Section: Discussionsupporting

confidence: 75%

“…The level of enzyme activity is an indicative of liver, bone and thyroid function [4]. Vitamin A has a specific effect on alkaline phosphatase activity, which is independent of keratinization [5]. Alkaline phosphatase activity is particularly abundant in intestine, kidney and bone [6].…”

Section: Introductionmentioning

confidence: 99%

“…Alkaline phosphatase has been studied as an indicator of intestinal epithelial cell differentiation [7]. Retinyl acetate has been shown to increase alkaline phosphatase activity in mouse skin, when applied topically [5]. Vitamin A also regulatcs the urothelial alkaline phosphatase activity [8].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microsomal alkaline phosphatase activity in retinol deficiency induced albino rats

Kaul

Krishnakantha

1993

Nahrung

View full text Add to dashboard Cite

The investigations showed a significant decrease in the alkaline phosphatase activity of retinol deficient liver (48.6%), kidney (65.8%) and spleen (61.9%), as compared to the controls (100%). An increase in Vmax and Km by 12 to 51.5% and 90.4 to 189%, respectively, was observed in all the tissues in the retinol deficient group, as compared to the controls. Subsequent freezing and thawing reduced the activity of alkaline phosphatase by 22.5 to 35.8% in the experimental group; whereas the reduction in the control group ranged from 8.8 to 21.5%. In the presence of lectins and detergents the activity of alkaline phosphatase decreased in both the groups to different levels.

show abstract

Section: Discussionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Microsomal alkaline phosphatase activity in retinol deficiency induced albino rats

Kaul

Krishnakantha

1993

Nahrung

View full text Add to dashboard Cite

show abstract

“…Three "broad spectrum" and specific alkaline phosphatase "inducers" were given to TB and HP: phenoharhital (24). vitamin A (19). and corticosteroid (10).…”

Section: Discussionmentioning

confidence: 99%

Hypophosphatasia: A Developmental Anomaly of Alkaline Phosphatase?

et al. 1976

View full text Add to dashboard Cite

This report deals with quantitative and qualitative investigations of alkaline phosphatase in two unrelated infants with the severe infantile form of hypophosphatasia. Both affected infants had no detectable leukocyte alkaline phosphatase activities and both sets of parents and one sibling tended to have low but variable leukocyte enzyme activities. Normal duodenal juice alkaline phosphatase activity was present in the one patient in whom it was measured and a wide range of variation in enzymic activity was observed in the stools. There was no significant difference in the stool enzyme activity between both patients with hypophosphatasia (42.01 +/- 9.77 U) and control infants (40.55 +/- 6.29 U). However, the heterozygous parents had values significantly lower than the control adults (2.10 +/- 0.47 as compared with 19.10 +/- 4.44 U). Intestinal bacteria did not contribute significantly to the stool alkaline phosphatase activity. Enzyme activity was present in the bile of one of the patients and nearly absent in that of the other. Three "inducers" of alkaline phosphatase were given to both patients (phenobarbital, vitamin A, and corticosteroid). No clinical improvement or rise in serum alkaline phosphatase activity was observed during the trial of therapy with these agents. However, a significant increase in the activity of serum acid phosphatase was demonstrated during the course of vitamin A administration, suggesting an in vivo action of vitamin A on the lysosomes through decreasing the stability of the membrane and releasing acid phosphatase to the serum. Quantitative determination of tissue alkaline phosphatases from autopsy tissues was highly variable: no activity was found in bone, lungs, or spleen of either infant; there was a discrepancy in liver and kidney alkaline phosphatase values (zero in one patient and present in the other) and activity was present in the intestinal mucosa of both. Qualitative analysis of kidney, liver, and intestinal alkaline phosphatase revealed some differences between the patients and control subjects in heat inactivation and phenylalanine inhibition (Table 3). Starch gel electrophoresis of the liver preparation of one patient disclosed a single band which had greater mobility than that of six control subjects matched for age. Liver extracts from a premature and from full term newborns showed two bands. The single band of the patient's liver enzyme corresponded to the newborn's fast moving component. In addition, the intestinal enzyme prepared from the same patient had an extra band when compared with age-matched control subjects.

show abstract

“…There is also an associated stimulation of RNA synthesis (Zachman, 1967;Lukacs, Christophers & Braun-Falco, 1971). Induced formation of a new iso-enzyme of alkaline phosphatase by mouse epidermal cells is stimulated by retinyl acetate (Riley & Spearman, 1968), and similar distributions of this enzyme induction after applications of other forms of vitamin A suggest that all act in the same manner on mouse epidermis. Formation of alkaline phosphatase in the scale epidermis indicates that the vitamin penetrates directly through the keratin layer.…”

Section: Discussionmentioning

confidence: 95%

Biological comparison of isomers and chemical forms of vitamin A (retinol)

Spearman

Jarrett

1974

Br J Dermatol

Self Cite

View full text Add to dashboard Cite

The effects were compared on mouse tail scale epidermis of vitamin A acid, acetate and alcohol (all as the trans isomer) and the as aldehyde by regular topical applications. Increases in epidermal thickness produced by difl'erent doses were compared statistically, and the induction of a granular layer and of epidermal alkaline phosphatase activity was demonstrated.The different forms and isomers of vitamin A have similar effects on the epidermis, but vary individually in activity. Retinoic acid and rraw5-retinyl acetate were very similar in their action when used in a water solubilizing vehicle. The fact that retinyl acetate is very much cheaper than retinoic acid is a point in favour of its use for the treatment of psoriasis.

show abstract

Vitamin A-Induced Synthesis of Alkaline Phosphatase

Cited by 22 publications

References 16 publications

Microsomal alkaline phosphatase activity in retinol deficiency induced albino rats

Microsomal alkaline phosphatase activity in retinol deficiency induced albino rats

Hypophosphatasia: A Developmental Anomaly of Alkaline Phosphatase?

Biological comparison of isomers and chemical forms of vitamin A (retinol)

Contact Info

Product

Resources

About