Vitamin B12 biosynthesis of Cetobacterium ceti isolated from the intestinal content of captive common bottlenose dolphins (Tursiops truncatus)

Suzuki, Akihiro; Shirakata, Chika; Anzai, H.; Sumiyama, Daisuke; Suzuki, Miwa

doi:10.1099/mic.0.001244

Cited by 10 publications

(5 citation statements)

References 62 publications

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“…The main metabolite of Cetobacterium is acetic acid, which can regulate the expression of insulin and promote the utilization of sugar by the body (Wang et al, 2021). Cetobacterium can also improve the body's metabolism of amino acids and promote the body's utilization of protein (Suzuki et al, 2022;Zhang et al, 2022). The results of this experiment show that ELM can improve the digestion and utilization of sugar and protein in the intestinal tract of spotted sea bass by increasing the activity of intestinal amylase and trypsin.…”

Section: B a Figurementioning

confidence: 82%

Effects of mulberry leaf extract on the intestinal health of spotted sea bass (Lateolabrax maculatus)

et al. 2023

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Mulberry leaf extract (ELM) has the functions of promoting growth, antioxidant, improving intestinal microbial composition, thus providing a potential solution the occurrence of fish intestinal diseases. Therefore, this experiment was conducted to explore the effects of ELM on intestinal health of spotted sea bass Lateolabrax maculatus. A total of 360 spotted sea bass (9.00 ± 0.02 g) were selected and randomly divided into 6 groups. Fish in each group were given feed with varying ELM concentration (0, 3, 6, 9, 12, 15 g/kg) for 52 days, respectively. Results show, dietary intake of 9 g/kg ELM increased the weight gain, specific growth ratio and feed intake of the spotted sea bass (P<0.05). Meanwhile, dietary intake of 9 g/kg ELM increased the activity of enteric trypsin, amylase and lipase (P<0.05). The enteric catalase activity was improved in fish fed with 3 g/kg ELM (P<0.05), while a limited effect of ELM on the activity of enteric superoxide dismutase, glutathione, and content of malonaldehyde was observed (P>0.05). ELM improved the morphology of fish intestine, as manifested in significant improvement in the length of intestinal villi, thereby increasing the surface area of the intestinal tract (P<0.05). Compared with the control group, dietary intake of ELM significantly increased the intestinal microbial ACE, Chao1, and Shannon indexes of fish (P<0.05), indicated that the intestinal microbial composition and the abundance of the dominant flora of fish were improved. The above results suggested that the dietary supplementation of about 9 g/kg ELM can improve the growth performance, enteric antioxidant capacity, and intestinal morphology and microbial composition, therefore improving the intestinal health of spotted sea bass. The research results provide a theoretical basis for the application of ELM in improving the enteric health of spotted sea bass, and providing a potential solution the occurrence of fish intestinal diseases.

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Section: B a Figurementioning

confidence: 82%

Effects of mulberry leaf extract on the intestinal health of spotted sea bass (Lateolabrax maculatus)

et al. 2023

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“…Functional studies revealed that Cetobacterium spp. synthesize vitamin B 12 , which has been shown to improve host resistance against pathogen infection [ 92 , 93 ]. The exact role of these Fusobacteriota in the microbiome of Coelopa frigida remains elusive, but its abundance in larval samples suggests a possible important role.…”

Section: Discussionmentioning

confidence: 99%

Microbiome Dynamics and Functional Composition in Coelopa frigida (Diptera, Coelopidae): Insights into Trophic Specialization of Kelp Flies

Bischof,

Bartolomaeus,

Löber

et al. 2024

Microb Ecol

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Coelopidae (Diptera), known as kelp flies, exhibit an ecological association with beached kelp and other rotting seaweeds. This unique trophic specialization necessitates significant adaptations to overcome the limitations of an algal diet. We aimed to investigate whether the flies’ microbiome could be one of these adaptive mechanisms. Our analysis focused on assessing composition and diversity of adult and larval microbiota of the kelp fly Coelopa frigida. Feeding habits of the larvae of this species have been subject of numerous studies, with debates whether they directly consume kelp or primarily feed on associated bacteria. By using a 16S rRNA metabarcoding approach, we found that the larval microbiota displayed considerably less diversity than adults, heavily dominated by only four operational taxonomic units (OTUs). Phylogenetic placement recovered the most dominant OTU of the larval microbiome, which is the source of more than half of all metabarcoding sequence reads, as an undescribed genus of Orbaceae (Gammaproteobacteria). Interestingly, this OTU is barely found among the 15 most abundant taxa of the adult microbiome, where it is responsible for less than 2% of the metabarcoding sequence reads. The other three OTUs dominating the larval microbiome have been assigned as Psychrobacter (Gammaproteobacteria), Wohlfahrtiimonas (Gammaproteobacteria), and Cetobacterium (Fusobacteriota). Moreover, we also uncovered a distinct shift in the functional composition between the larval and adult stages, where our taxonomic profiling suggests a significant decrease in functional diversity in larval samples. Our study offers insights into the microbiome dynamics and functional composition of Coelopa frigida.

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“…Cetobacterium (ASV015), a member of Fusobacteriaceae like the highly abundant Fusobacterium , is not common within the gut microbiota of terrestrial animals but has been found in other native Australian mammals such as Tasmanian devils (Cheng et al, 2015) and platypus (Dungan & Thomas, 2023). This genus can assist in carbohydrate and peptide fermentation and is suggested to produce vitamin B 12 , which is essential for the biosynthesis of heme, a component of hemoglobin and myoglobin, that cannot be produced by mammals (Bhute et al, 2020; Suzuki et al, 2022). Streptococcus (ASV022), on the other hand, is associated with disease in echidnas (McOrist & Smales, 1986).…”

Section: Discussionmentioning

confidence: 99%

Gut microbiota in the short‐beaked echidna (Tachyglossus Aculeatus) shows stability across gestation

Buthgamuwa,

Fenelon,

Roser

et al. 2023

MicrobiologyOpen

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Indigenous gut microbial communities (microbiota) play critical roles in health and may be especially important for the mother and fetus during pregnancy. Monotremes, such as the short‐beaked echidna, have evolved to lay and incubate an egg, which hatches in their pouch where the young feeds. Since both feces and eggs pass through the cloaca, the fecal microbiota of female echidnas provides an opportunity for vertical transmission of microbes to their offspring. Here, we characterize the gut/fecal microbiome of female short‐beaked echidnas and gain a better understanding of the changes that may occur in their microbiome as they go through pregnancy. Fecal samples from four female and five male echidnas were obtained from the Currumbin Wildlife Sanctuary in Queensland and sequenced to evaluate bacterial community structure. We identified 25 core bacteria, most of which were present in male and female samples. Genera such as Fusobacterium, Bacteroides, Escherichia‐Shigella, and Lactobacillus were consistently abundant, regardless of sex or gestation stage, accounting for 58.00% and 56.14% of reads in male and female samples, respectively. The echidna microbiome remained stable across the different gestation stages, though there was a significant difference in microbiota composition between male and female echidnas. This study is the first to describe the microbiome composition of short‐beaked echidnas across reproductive phases and allows the opportunity for this novel information to be used as a metric of health to aid in the detection of diseases triggered by microbiota dysbiosis.

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Vitamin B12 biosynthesis of Cetobacterium ceti isolated from the intestinal content of captive common bottlenose dolphins (Tursiops truncatus)

Cited by 10 publications

References 62 publications

Effects of mulberry leaf extract on the intestinal health of spotted sea bass (Lateolabrax maculatus)

Effects of mulberry leaf extract on the intestinal health of spotted sea bass (Lateolabrax maculatus)

Microbiome Dynamics and Functional Composition in Coelopa frigida (Diptera, Coelopidae): Insights into Trophic Specialization of Kelp Flies

Gut microbiota in the short‐beaked echidna (Tachyglossus Aculeatus) shows stability across gestation

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