Vitamin B12 Deficiency Across Three Generations Adversely Influences Long-chain Polyunsaturated Fatty Acid Status and Cardiometabolic Markers in Rats

Khaire, Amrita; Rathod, Richa; Kale, Anvita; Joshi, Sadhana

doi:10.1016/j.arcmed.2016.10.006

Cited by 6 publications

(6 citation statements)

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“…Although vitamin B 12 deficiency is a serious worldwide health issue, nonclinical research has been primarily limited to mammalian models (22)(23)(24)(25)(26)(27)(28). Zebrafish (Danio rerio) are a potentially powerful model system with which to study vitamin B 12 metabolism and biology.…”

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confidence: 99%

Functional and phylogenetic characterization of noncanonical vitamin B12–binding proteins in zebrafish suggests involvement in cobalamin transport

Benoit

Stanton

Tartanian

et al. 2018

Journal of Biological Chemistry

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Edited by Ruma Banerjee In humans, transport of food-derived cobalamin (vitamin B 12) from the digestive system into the bloodstream involves three paralogous proteins: transcobalamin (TC), haptocorrin (HC), and intrinsic factor (IF). Each of these proteins contains two domains, an ␣-domain and a ␤-domain, which together form a cleft in which cobalamin binds. Zebrafish (Danio rerio) are thought to possess only a single cobalamin transport protein, referred to as Tcn2, which is a transcobalamin homolog. Here, we used CRISPR/Cas9 mutagenesis to create null alleles of tcn2 in zebrafish. Fish homozygous for tcn2-null alleles were viable and exhibited no obvious developmentally or behaviorally abnormal phenotypes. For this reason, we hypothesized that previously unidentified cobalamin-carrier proteins encoded in the zebrafish genome may provide an additional pathway for cobalamin transport. We identified genes predicted to code for two such proteins, Tcn-beta-a (Tcnba) and Tcn-beta-b (Tcnbb), which differ from all previously characterized cobalamin transport proteins as they lack the ␣-domain. These ␤-domainonly proteins are representative of an undescribed class of cobalamin-carrier proteins that are highly conserved throughout the ray-finned fishes. We observed that the genes encoding the three cobalamin transport homologs, tcn2, tcnba, and tcnbb, are expressed in unique spatial and temporal patterns in the developing zebrafish. Moreover, exogenously expressed recombinant Tcnba and Tcnbb bound cobalamin with high affinity, comparable with binding by full-length Tcn2. Taken together, our results suggest that this noncanonical protein structure has evolved to fully function as a cobalamin-carrier protein, thereby allowing for a compensatory cobalamin transport mechanism in the tcn2 ؊/؊ zebrafish. Vitamin B 12 , also referred to as cobalamin, is a micronutrient that acts as a cofactor for two enzymes: methionine synthase, This article contains Figs. S1-S4 and Table S1.

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confidence: 99%

Functional and phylogenetic characterization of noncanonical vitamin B12–binding proteins in zebrafish suggests involvement in cobalamin transport

Benoit

Stanton

Tartanian

et al. 2018

Journal of Biological Chemistry

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“…As expected, GSD patients had significantly higher triglyceride concentrations than healthy controls ( p < 0.0001, Table 1 ). Some associations have been described between vitamin B 12 status and triglyceride/lipid metabolism in human and animal studies [ 34 , 35 , 36 , 37 , 38 ]. Thus, we investigated whether associations exist between vitamin B 12 and triglycerides concentrations in healthy controls and GSD patients.…”

Section: Resultsmentioning

confidence: 99%

Elevated Plasma Vitamin B12 in Patients with Hepatic Glycogen Storage Diseases

Hinkel

Schmitt

Wurm

et al. 2020

JCM

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Background: Hepatic glycogen storage diseases (GSDs) are inborn errors of metabolism affecting the synthesis or breakdown of glycogen in the liver. This study, for the first time, systematically assessed vitamin B12 status in a large cohort of hepatic GSD patients. Methods: Plasma vitamin B12, total plasma homocysteine (tHcy) and methylmalonic acid concentrations were measured in 44 patients with hepatic GSDs and compared to 42 healthy age- and gender-matched controls. Correlations of vitamin B12 status with different disease markers of GSDs (including liver transaminase activities and triglycerides) as well as the vitamin B12 intake were studied. Results: GSD patients had significantly higher plasma vitamin B12 concentrations than healthy controls (p = 0.0002). Plasma vitamin B12 concentration remained elevated in GSD patients irrespective of vitamin B12 intake. Plasma vitamin B12 concentrations correlated negatively with triglyceride levels, whereas no correlations were detected with liver transaminase activities (GOT and GPT) in GSD patients. Merging biomarker data of healthy controls and GSD patients showed a positive correlation between vitamin B12 status and liver function, which suggests complex biomarker associations. A combined analysis of biomarkers permitted a reliable clustering of healthy controls versus GSD patients. Conclusions: Elevated plasma concentration of vitamin B12 (irrespective of B12 intake) is a common finding in patients with hepatic GSD. The negative correlation of plasma vitamin B12 with triglyceride levels suggests an influence of metabolic control on the vitamin B12 status of GSD patients. Elevated vitamin B12 was not correlated with GOT and GPT in our cohort of GSD patients. Merging of data from healthy controls and GSD patients yielded positive correlations between these biomarkers. This apparent dichotomy highlights the intrinsic complexity of biomarker associations and argues against generalizations of liver disease and elevated vitamin B12 in blood. Further studies are needed to determine whether the identified associations are causal or coincidental, and the possible impact of chronically elevated vitamin B12 on GSD.

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“…21 Estudo realizado em âmbito experimental com ratos refere que uma dieta com deficiência de vitamina B12 pode favorecer a diminuição dos níveis de ácido eicosapentaenóico (EPA) e ácido docosahexaenóico (DHA) hepático e no plasma desses animais. 22 A deficiência de cianocobalamina representa um problema de saúde para o público vegetariano, especialmente os veganos, se não houver suplementação ou consumo habitual de alimentos fortificados. 23 Embora alguns alimentos, como produtos derivados da soja, algas marinhas e vegetais fermentados, sejam apontados como fontes de vitamina B12, na realidade é inadequada essa afirmativa, uma vez que a cobalamina que entra na sua constituição é um análogo inativo de vitamina B12.…”

Section: Discussionunclassified

Aceitabilidade De Barra De Cereal Fonte De Proteína E Vitamina B12 Por Ovo-Lacto-Vegetarianos

Santos

Costa

2018

DEMETRA

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Objetivos: Considerando que a dieta vegetariana, quando não balanceada, pode desencadear carências nutricionais importantes, entre elas de vitamina B12 e proteína, objetivou-se formular barra de cereal fonte de vitamina B12 e proteína e verificar a aceitabilidade por ovo-lacto-vegetarianos de uma instituição religiosa. Metodologia: As barras de cereal foram elaboradas com cereal fortificado, whey protein, castanha de caju, glucose de milho e água. A proporção de ingredientes foi estabelecida de modo a caracterizá-las como alimento fonte de proteína e vitamina B12. A análise sensorial foi realizada para os requisitos sabor, aroma, cor e textura, por meio de escala hedônica estruturada verbal de 9 pontos. Resultados: A barra de cereal formulada atingiu as exigências da legislação, para proteína e vitamina B12. A barra obteve aceitação próxima ou superior a 90% em todas as variáveis estudadas, mas o fator textura apresentou menor aceitação quando comparado aos demais atributos. Os indivíduos categorizados em ensino fundamental e o sexo feminino demonstraram maior aceitação global da preparação. Discussão: A dieta vegetariana, quando não balanceada, pode desencadear carências nutricionais, e em algumas ocasiões a ingestão de suplementos e alimentos fortificados é necessária. Estudos demonstram que a textura é fator limitante na elaboração de barras de cereal, que são bem aceitas, em especial pelo público feminino, e podem ser utilizadas casualmente como opção de lanche. Conclusão: A barra de cereal teve boa aceitação pelo público ovo-lacto-vegetariano em todas as variáveis estudadas; assim, pode vir a ser adotada como opção de lanche rápido.DOI: 10.12957/demetra.2018.32101

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Vitamin B12 Deficiency Across Three Generations Adversely Influences Long-chain Polyunsaturated Fatty Acid Status and Cardiometabolic Markers in Rats

Cited by 6 publications

References 56 publications

Functional and phylogenetic characterization of noncanonical vitamin B12–binding proteins in zebrafish suggests involvement in cobalamin transport

Functional and phylogenetic characterization of noncanonical vitamin B12–binding proteins in zebrafish suggests involvement in cobalamin transport

Elevated Plasma Vitamin B12 in Patients with Hepatic Glycogen Storage Diseases

Aceitabilidade De Barra De Cereal Fonte De Proteína E Vitamina B12 Por Ovo-Lacto-Vegetarianos

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