Vitamin B12 Enriched in Spinach and its Effects on Gut Microbiota

Zheng, Yiqing; Xiang, Shasha; Zhang, Hongxing; Ye, Kun; Zhang, Yalin; Yin, Guofu; Feng, Xiao; Bao, Xuan; Chen, Jie; Zhu, Xuan

doi:10.1021/acs.jafc.0c07597

Cited by 14 publications

(9 citation statements)

References 35 publications

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“…In vitro study suggested that the addition of cobalamins increases the generation of SCFAs, especially butyrate and propionic acid ( 218 ). Another in vitro study indicated that low-dose cyanocobalamin-enriched spinach could increase the generation of butyrate and acetate ( 228 ). In mice, a reduction of SCFAs has been observed in dietary vitamin B12 restriction ( 229 ).…”

Section: Vitamin B12mentioning

confidence: 99%

Intermediate role of gut microbiota in vitamin B nutrition and its influences on human health

Wan

Zheng²,

Zhu³

et al. 2022

Front. Nutr.

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Vitamin B consists of a group of water-soluble micronutrients that are mainly derived from the daily diet. They serve as cofactors, mediating multiple metabolic pathways in humans. As an integrated part of human health, gut microbiota could produce, consume, and even compete for vitamin B with the host. The interplay between gut microbiota and the host might be a crucial factor affecting the absorbing processes of vitamin B. On the other hand, vitamin B supplementation or deficiency might impact the growth of specific bacteria, resulting in changes in the composition and function of gut microbiota. Together, the interplay between vitamin B and gut microbiota might systemically contribute to human health. In this review, we summarized the interactions between vitamin B and gut microbiota and tried to reveal the underlying mechanism so that we can have a better understanding of its role in human health.

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Section: Vitamin B12mentioning

confidence: 99%

Intermediate role of gut microbiota in vitamin B nutrition and its influences on human health

Wan

Zheng²,

Zhu³

et al. 2022

Front. Nutr.

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“…Recent human clinical intervention trials have assessed diverse aspects of SPI intake, including anthropometric measures, muscle fitness, metabolic profiles, arterial stiffness, and urinary biomarkers [5,[34][35][36], extending prior research on the health benefits of green leafy vegetables and the functional properties of spinach-derived phytochemicals and bioactives [37][38][39][40][41]. We reported that long-term feeding of freeze-dried SPI at 10% w/w in the diet for 26 weeks exhibited significant antitumor efficacy in the Pirc model, resulting in >60% reduced tumor multiplicity in the colon and small intestine [6].…”

Section: Discussionmentioning

confidence: 99%

Metabolomics of Acute vs. Chronic Spinach Intake in an Apc–Mutant Genetic Background: Linoleate and Butanoate Metabolites Targeting HDAC Activity and IFN–γ Signaling

Chen

Li²,

Neja³

et al. 2022

Cells

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There is growing interest in the crosstalk between the gut microbiome, host metabolomic features, and disease pathogenesis. The current investigation compared long–term (26 week) and acute (3 day) dietary spinach intake in a genetic model of colorectal cancer. Metabolomic analyses in the polyposis in rat colon (Pirc) model and in wild–type animals corroborated key contributions to anticancer outcomes by spinach–derived linoleate bioactives and a butanoate metabolite linked to increased α–diversity of the gut microbiome. Combining linoleate and butanoate metabolites in human colon cancer cells revealed enhanced apoptosis and reduced cell viability, paralleling the apoptosis induction in colon tumors from rats given long–term spinach treatment. Mechanistic studies in cell–based assays and in vivo implicated the linoleate and butanoate metabolites in targeting histone deacetylase (HDAC) activity and the interferon–γ (IFN–γ) signaling axis. Clinical translation of these findings to at–risk patients might provide valuable quality–of–life benefits by delaying surgical interventions and drug therapies with adverse side effects.

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“…Detection conditions: column—ZORBAX SB-Aq (4.6 × 250 mm 5-micron); mobile phase—0.025% phosphate aqueous solution (pH = 2.8), acetonitrile = 95:5; flow rate—1.0 mL/min; injection volume—20 μL; detection wavelength—210 nm; column temperature—30 °C [ 10 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of Two Kinds of Extracts of Cistanche deserticola on Intestinal Microbiota and Its Metabolism

Zhang

Su³

et al. 2022

Foods

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Cistanche deserticola belongs to the Liedang family. Known as “desert ginseng”, it has high medicinal value and plays important roles in endocrine regulation, neuroprotection, immune regulation, and other processes. Some studies have shown that single substances such as polysaccharides and phenylethanolside can affect intestinal microbiota, but few studies have studied the synergistic effect of various components in Cistanche deserticola extracts on intestinal microbiota. Therefore, in this study, through an in vitro digestion model (Changdao Moni, CDMN) combined with 16S rRNA gene amplification sequencing technology and untargeted metabolomics technology, it was found that the two extracts all had significant effects on the enteric cavity and mucosal flora. Both extracts inhibited Bacteroides in the intestinal cavity and Parabacteroides and Ruminococcus 2 in the intestinal mucosa and promoted Bifidobacterium and Prevotella in the intestinal cavity and Megasphaera in the intestinal mucosa. The aqueous extract also inhibited Phascolarctobacterium. Both extracts also significantly increased the production of short-chain fatty acids, especially butyrate. The intake of extract had significant effects on the metabolic pathways related to amino acids and lipids. Indoles were upregulated by the aqueous extract but downregulated by the alcohol extract. In addition, the extract also had a significant effect on the hemolytic phosphorus esters. In conclusion, the two kinds of extracts have different effects on intestinal microbiota and its metabolism. This study provides guiding significance for the edibility and food development of Cistanche deserticola.

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Vitamin B12 Enriched in Spinach and its Effects on Gut Microbiota

Cited by 14 publications

References 35 publications

Intermediate role of gut microbiota in vitamin B nutrition and its influences on human health

Intermediate role of gut microbiota in vitamin B nutrition and its influences on human health

Metabolomics of Acute vs. Chronic Spinach Intake in an Apc–Mutant Genetic Background: Linoleate and Butanoate Metabolites Targeting HDAC Activity and IFN–γ Signaling

Effects of Two Kinds of Extracts of Cistanche deserticola on Intestinal Microbiota and Its Metabolism

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