Vitamin B12-folic acid supplementation regulates neuronal immediate early gene expression and improves hippocampal dendritic arborization and memory in old male mice

Barman, Bhabotosh; Kushwaha, Akanksha; Thakur, M. K.

doi:10.1016/j.neuint.2021.105181

Cited by 13 publications

(7 citation statements)

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“…Of the 35 animal model studies, 14 were conducted with mice (C57BL/6J, CBA/J, Swiss Albino, C57BL/6, AxinFu/þ, and/or INS-GAS) [33–46] and 21 with rats (Sprague-Dawley, Long-Evans and Wistar) [47–67]. The route of supplement administration included adding to water [33, 34, 43] or diet (chow) [35–38, 40, 42–45, 47, 49–56, 58, 59, 61–67], oral gavage [41] and subcutaneous injection [57]. Nineteen studies supplemented only folic acid [35, 39, 41–45, 48, 50, 54, 56, 59, 60, 62, 64–67], four only choline [33, 57, 61, 63], three only betaine [47, 49, 55], three only methionine [37, 38, 58], and six studies used a mix of methyl-donor supplements [34, 36, 40, 51–53].…”

Section: Resultsmentioning

confidence: 99%

“…The route of supplement administration included adding to water [33, 34, 43] or diet (chow) [35–38, 40, 42–45, 47, 49–56, 58, 59, 61–67], oral gavage [41] and subcutaneous injection [57]. Nineteen studies supplemented only folic acid [35, 39, 41–45, 48, 50, 54, 56, 59, 60, 62, 64–67], four only choline [33, 57, 61, 63], three only betaine [47, 49, 55], three only methionine [37, 38, 58], and six studies used a mix of methyl-donor supplements [34, 36, 40, 51–53]. Samples included maternal placenta and diverse fetal or own animal tissues such as liver, brain, kidney, adipose tissue.…”

Section: Resultsmentioning

confidence: 99%

“…Of the 35 animal model studies, 14 were conducted with mice (C57BL/6J, CBA/J, Swiss Albino, C57BL/6, AxinFu/þ, and/or INS-GAS) [33][34][35][36][37][38][39][40][41][42][43][44][45][46] and 21 with rats (Sprague-Dawley, Long-Evans and Wistar) . The route of supplement administration included adding to water [33,34,43] or diet (chow) [35-38, 40, 42-45, 47, 49-56, 58, 59, 61-67], oral gavage [41] and subcutaneous injection [57].…”

Section: Animal Model Studiesmentioning

confidence: 99%

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Impact of Methyl-Donor Micronutrient Supplementation on DNA Methylation Patterns: A Systematic Review and Meta-Analysis of in vitro, Animal, and Human Studies

da Mota,

Ribeiro,

Carvalho

et al. 2023

Lifestyle Genomics

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Background: DNA methylation patterns are directly associated with diverse metabolic disorders. The status of methyl-donor micronutrients has been associated with DNA methylation levels, and altered ingestion of folate, choline, betaine, B vitamins and methionine may impact genes both globally and at the level of promoter regions. Despite this, the role of methyl-donor micronutrient supplementation on DNA methylation profiles is currently unclear. Objectives: The aims of this systematic review and meta-analysis were to identify and synthesize the evidence about methyl-donor nutrient supplementation on DNA methylation. Methods: A systematic literature search was performed in Medline, Embase, Scopus, and Web of Science databases with a combination of terms related to DNA methylation assessment, supplementation, and methyl-donor nutrients. Studies (in vitro, animal models, or human clinical trials) were included if DNA methylation levels after any kind of methyl-donor micronutrient supplementation or treatment was investigated. Studies were assessed for bias using Revised Cochrane risk-of-bias tool for randomized trials, risk-of-bias in Non-randomized Studies of Interventions or Systematic Review Centre for Laboratory Animal Experimentation tools. Data were extracted from studies measuring DNA methylation levels in any sample or tissue, following any kind of methyl-donor micronutrient supplementation or treatment. Separate random-effects meta-analyses were performed for animal model studies and human clinical trials that examined the effects of folic acid supplementation on DNA methylation. Results: Fifty-seven studies were included in this systematic review: 18 human clinical trials, 35 in animal model, and 4 in vitro studies. Concerning overall risk of bias, most of the studies were classified as “high risk” or “some concerns.” Meta-analysis with meta-regression from studies in animal models showed that folic acid dose significantly affected DNA methylation and that high and very high doses showed increases in DNA methylation when compared to low doses. However, meta-analysis of human clinical trials showed that folic acid supplementation did not promote significant changes in DNA methylation when compared to placebo. Conclusion: Folic acid supplementation may change global DNA methylation levels in animals supplemented with high, as compared to low, doses. Heterogeneity in studies and supplementation protocols make it difficult to establish clinical recommendations. However, these effects, even if small, might be of clinical importance in the management of patients with diseases related to DNA hypomethylation.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Animal Model Studiesmentioning

confidence: 99%

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Impact of Methyl-Donor Micronutrient Supplementation on DNA Methylation Patterns: A Systematic Review and Meta-Analysis of in vitro, Animal, and Human Studies

da Mota,

Ribeiro,

Carvalho

et al. 2023

Lifestyle Genomics

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“…Mouse interaction with objects is measured by mouse head entry in the periphery. Time spent with the novel object was calculated as TNovel/(TNovel + TFamiliar), time spent with the familiar object was calculated as TFamiliar/(TNovel + TFamiliar), and Discrimination Index (DI) for the novel object was calculated as (TNovel -TFamiliar)/(TNovel + TFamiliar) [31].…”

Section: Novel Object Recognition Testmentioning

confidence: 99%

Differential alterations in the expression of AMPA receptor and its trafficking proteins in the hippocampus is associated with recognition memory impairment the rotenone-Parkinson's disease mouse model: neuroprotective role of Bacopa monneiri extract CDRI 08

Gupta,

Prasad

2024

Preprint

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Parkinson's disease (PD), one of the age-associated neurodegenerative disorders, is associated with motor abnormalities. In addition, the PD leads to gradual deterioration of cognitive decline with advancing age. Apart from the hallmark accumulation of α-Synuclein (α-Syn) in the substantia nigra pars compacta (SNPc) dopaminergic neurons leading to their loss, the precise molecular basis of the PD-induced cognitive decline and the therapeutic intervention is not yet understood. In the current study, our Western blotting and qRT-PCR data from the rotenone-induced PD mouse model reveal that the PD-induced recognition memory loss is associated with significant upregulation of the GluR1 subunit and downregulation of Glur2 subunit of the AMPA receptor in the hippocampus of rotenone-treated mice as compared to the vehicle control mice. Our data also reveal that its trafficking proteins are significantly upregulated in hippocampus (DG, CA3, and CA1 regions) of PD mice compared to the vehicle control. Bacopa monnieri extract (BME) called CDRI-08 at the dose of 200mg/Kg BW has shown its abilities to reverse the expression of AMPA receptor subunit and its trafficking protein in differential manner depending on whether the BME treatment was given prior to or after the rotenone treatment to mice. Our data clearly suggest that the pre treatment given to mice reverses the expression of the memory associated genes compared to the treatment after rotenone administration. Our study further suggests that the above changes in the gene expression in PD affected hippocampus are associated with modulation of their transcriptional machinery by BDNF and CREB. Expression of both are significantly lowered in the hippocampus the rotenone-treated mice in comparison to their levels in the control mice. The mice treated first with CDRI-08 significantly upregulated their expression compared to rotenone-treated mice, and when compared with mice treated after the rotenone treatment. Our results provide the evidence for the underlying molecular basis of cognitive decline in PD in rotenone-PD model and the possible mechanisms for the neuroprotective role of Bacopa monnieri extract CDRI-08 which shows its therapeutic potential for the PD-induced cognitive impairment.

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“…These abnormalities are also commonly found in the aging brain. Downregulated synaptic plasticity-related immediate-early gene expression also plays a major role in age-associated cognitive impairment ( Barman et al, 2021 ). Oxidative stress-mediated unrepaired DNA damage at those genes promoters causes downregulation of their expression ( Ionescu-Tucker and Cotman, 2021 ).…”

Section: Oxidative Stress In the Aging Brainmentioning

confidence: 99%

Oxidative stress-mediated memory impairment during aging and its therapeutic intervention by natural bioactive compounds

Singh

Barman

Thakur

2022

Front. Aging Neurosci.

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Aging and associated neurodegenerative diseases are accompanied by the decline of several brain functions including cognitive abilities. Progressive deleterious changes at biochemical and physiological levels lead to the generation of oxidative stress, accumulation of protein aggregates, mitochondrial dysfunctions, loss of synaptic connections, and ultimately neurodegeneration and cognitive decline during aging. Oxidative stress that arises due to an imbalance between the rates of production and elimination of free radicles is the key factor for age-associated neurodegeneration and cognitive decline. Due to high energy demand, the brain is more susceptible to free radicals-mediated damages as they oxidize lipids, proteins, and nucleic acids, thereby causing an imbalance in the homeostasis of the aging brain. Animal, as well as human subject studies, showed that with almost no or few side effects, dietary interventions and plant-derived bioactive compounds could be beneficial to recovering the memory or delaying the onset of memory impairment. As the plant-derived bioactive compounds have antioxidative properties, several of them were used to recover the oxidative stress-mediated changes in the aging brain. In the present article, we review different aspects of oxidative stress-mediated cognitive change during aging and its therapeutic intervention by natural bioactive compounds.

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Vitamin B12-folic acid supplementation regulates neuronal immediate early gene expression and improves hippocampal dendritic arborization and memory in old male mice

Cited by 13 publications

References 74 publications

Impact of Methyl-Donor Micronutrient Supplementation on DNA Methylation Patterns: A Systematic Review and Meta-Analysis of in vitro, Animal, and Human Studies

Impact of Methyl-Donor Micronutrient Supplementation on DNA Methylation Patterns: A Systematic Review and Meta-Analysis of in vitro, Animal, and Human Studies

Differential alterations in the expression of AMPA receptor and its trafficking proteins in the hippocampus is associated with recognition memory impairment the rotenone-Parkinson's disease mouse model: neuroprotective role of Bacopa monneiri extract CDRI 08

Oxidative stress-mediated memory impairment during aging and its therapeutic intervention by natural bioactive compounds

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