Vitamin B12: For more than just the treatment of megaloblastic anemia?

Pardo-Cabello, Alfredo José; Manzano-Gamero, Victoria; Puche-Cañas, E

doi:10.1016/j.rceng.2022.11.004

Cited by 5 publications

(3 citation statements)

References 29 publications

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“…vs c e (4) lnq e vs lnc e (5) Molecules 2024, 29, x FOR PEER REVIEW 7 of 20 0.05 mg mL −1 . After 0.075 g of IONPs, we observe a plateau, which confirms the fact that this mass of IONPs was enough to remove 0.05 mg mL −1 of cyanocobalamin.…”

Section: Adsorption Isothermsmentioning

confidence: 99%

“…A stable form of vitamin B12 that does not occur naturally is cyanocobalamin [3]. Vitamin B12 is involved in many physiological processes, including the formation and development of the myelin sheath, red blood cell maturation, and nucleic acid synthesis [4][5][6][7]. Adequate vitamin B12 can be obtained from a well-selected diet rich in animal products (veal, beef, poultry, fish, seafood, eggs, and dairy products) [8,9].…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Cyanocobalamin (Vitamin B12) Sorption onto Mesoporous Superparamagnetic Iron Oxide Nanoparticles

Flieger,

Żuk,

Pasieczna-Patkowska

et al. 2024

Molecules

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The techniques used to detect and quantify cyanocobalamin (vitamin B12) vary considerably in terms of detection sensitivity, from the most sensitive, based on radioisotopes and mass spectrometry (MS) with limits of detection (LOD) in fg mL−1, to fluorescence (FL) and surface plasmon resonance (SPR) biosensors with LOD values in the range of a few µg mL−1. For accurate quantification of an analyte present at trace levels in complex biological matrices, a selective separation and enrichment step is required to overcome matrix interferences and ensure sufficient detection sensitivity. In this study, iron oxide magnetic nanoparticles (IONPs) were used for the extraction and initial preconcentration of cyanocobalamin (vitamin B12). In the dependence of the magnetization on the H-field (hysteresis loop), no coercivity and remanence values were found at 300 K, indicating the superparamagnetic properties of the tested IONPs. Perfluorinated acids were used as amphiphilic agents to allow the sorption of cyanocobalamin onto the IONPs. FT-IR/ATR spectroscopy was used to confirm the sorption of cyanocobalamin on the IONPs. The influence of the addition of a homologous series of perfluorinated acids such as trifluoroacetic acid (TFAA), heptafluorobutyric acid (HFBA), and trichloroacetic acid (TCAA) to the extraction mixture was tested considering their type, mass, and time required for effective sorption. The adsorption kinetics and isotherm, described by the Freundlich and Langmuir equations, were analyzed. The maximum adsorption capacity (qm) exceeded 6 mg g−1 and was 8.9 mg g−1 and 7.7 mg g−1 for HFBA and TCAA, respectively, as the most efficient additives. After the desorption process using aqueous KH2PO4 solution, the sample was finally analyzed spectrophotometrically and chromatographically. The IONP-based method was successfully applied for the isolation of cyanocobalamin from human urine samples. The results showed that the developed approach is simple, cheap, accurate, and efficient for the determination of traces of cyanocobalamin in biological matrices.

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Section: Adsorption Isothermsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of Cyanocobalamin (Vitamin B12) Sorption onto Mesoporous Superparamagnetic Iron Oxide Nanoparticles

Flieger,

Żuk,

Pasieczna-Patkowska

et al. 2024

Molecules

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“…Cyanocobalamin (vitamin B12) is a methylation factor cofactor of enzymes of hematopoiesis and metabolism of organic acids and has a lipotropic effect (Green & Miller, 2020;Kather et al, 2020;Bhowmik et al, 2021;Pardo-Cabello et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Research on the embryotoxic effect and carcinogenicity of the drug “BTF plus” – a means for normalizing metabolic processes in animals and poul-try

Sachuk,

Gutyj,

Radzyhovskyi

et al. 2023

Ukr. Jour. of Vet. and Agr. Sci.

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Laboratory studies were conducted to determine the embryotoxic effect and carcinogenicity of the veterinary drug “BTF plus” on white rats and white mice. The drug “BTF plus” is a complex vitamin-mineral drug based on butophosphane, L-carnitine, and cyanocobalamin, which is used to normalize and correct metabolic processes in animals and poultry. The drug is used for various types of animals and poultry as a stimulating, tonic and general strengthening agent for obstetric pathologies (complicated childbirth, postpartum complications, paresis, eclampsia, sexual cycle disorders); metabolic disorders caused by irrational feeding, malnutrition, asthenic syndrome, etc.; anemia with helminthiasis; secondary anemias, as an additional means in the treatment of magnesium and calcium deficiency; to increase muscle activity, with significant loads, overstrain and exhaustion in animals; to increase the body's resistance to various pathogens; to stimulate growth, development and live weight gain in young animals and poultry; as an additional means in the treatment of diseases caused by various factors (infectious and non-infectious origin). The drug “BTF plus”, under the conditions of subcutaneous administration to pregnant female rats in doses (based on the absolute weight of the drug) of 200.0 and 2000.0 mg/kg of body weight, does not cause death and pathological changes in embryos do not have an embryotoxic and teratogenic effect since indicators of total, preimplantation, and postimplantation embryonic lethality in rats of the experimental groups had no significant differences compared to indicators in control and also did not show changes in the weight of the placenta, fetuses, and their cranio-caudal size. The drug “BTF plus”, under conditions of 5-day subcutaneous administration to white mice in doses (based on the absolute weight of the drug) of 200.0 and 2000.0 mg/kg of body weight, does not show a carcinogenic effect (during microscopic studies, the proportion of polychromatophilic erythrocytes was not probable deviations between themselves and was 0.117-0.133%, which is within the normal range of up to 0.2 %). Further studies will be the next stage of pre-registration tests aimed at studying the ecotoxicity of “BTF plus”, which is a mandatory material of the “Safety and residue studies” section of the dossier for this drug.

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Yttrium-encapsulated fullerene (Y@C80) mono-doping with As, Bi, Sb, and P for the enhanced sensing of methylmalonic acid (MMA) as a biomarker for vitamin B12 deficiency: A DFT study

Ekpong,

Ogbogu,

Edet

et al. 2024

Computational and Theoretical Chemistry

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Vitamin B12: For more than just the treatment of megaloblastic anemia?

Cited by 5 publications

References 29 publications

Optimization of Cyanocobalamin (Vitamin B12) Sorption onto Mesoporous Superparamagnetic Iron Oxide Nanoparticles

Optimization of Cyanocobalamin (Vitamin B12) Sorption onto Mesoporous Superparamagnetic Iron Oxide Nanoparticles

Research on the embryotoxic effect and carcinogenicity of the drug “BTF plus” – a means for normalizing metabolic processes in animals and poul-try

Yttrium-encapsulated fullerene (Y@C80) mono-doping with As, Bi, Sb, and P for the enhanced sensing of methylmalonic acid (MMA) as a biomarker for vitamin B12 deficiency: A DFT study

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