Vitamin C Mitigates Oxidative Stress and Behavioral Impairments Induced by Deltamethrin and Lead Toxicity in Zebrafish

Abstract

Environmental contamination from toxic metals and pesticides is an issue of great concern due to their harmful effects to human health and the ecosystems. In this framework, we assessed the adverse effects when aquatic organisms are exposed to toxicants such as deltamethrin (DM) and lead (Pb), alone or in combination, using zebrafish as a model. Moreover, we likewise evaluated the possible protective effect of vitamin C (VC) supplementation against the combined acute toxic effects of the two toxicants. Juvenile zebrafish were exposed to DM (2 μg L−1) and Pb (60 μg L−1) alone and in combination with VC (100 μg L−1) and responses were assessed by quantifying acetylcholinesterase (AChE) activity, lipid peroxidation (MDA), some antioxidant enzyme activities (SOD and GPx), three-dimension locomotion responses and changes of elements concentrations in the zebrafish body. Our results show that VC has mitigative effects against behavioral and biochemical alterations induced by a mixture of contaminants, demonstrating that it can be used as an effective antioxidant. Moreover, the observations in the study demonstrate zebrafish as a promising in vivo model for assessing the neuroprotective actions of bioactive compounds.

Assessing Anti-Social and Aggressive Behavior in a Zebrafish (Danio rerio) Model of Parkinson’s Disease Chronically Exposed to Rotenone

“…The normality and distribution were determined by Shapiro–Wilk test with Graph Pad Prism software (v 9.1.0.221, San Diego, CA, USA). Subsequently, multiple comparisons between the initial behavior and the days of testing within the groups were performed with one-way ANOVA followed by Dunnett’s test [ 21 , 22 ]. Trends were generated using OriginPro software (v 9.3-2016, OriginLab Corporation, Northampton, MA, USA).…”

In silico prediction aided preparation of antioxidant soybean peptides by enzymatic hydrolysis for ameliorating lead exposure-induced toxicity

“…6 Many studies have shown that the antioxidant bioactive substances can protect against Pb exposure-induced toxicity in animal models. 7,8 It was suggested that the combination of chelating agents and antioxidants can better manage Pb toxicity. 9 However, the commonly used chelators in heavy metal treatment can cause serious side effects.…”

Glycyrrhiza Extract and Curcumin Alleviates the Toxicity of Cadmium via Improving the Antioxidant and Immune Functions of Black Goats

“…After Cd entered liver cells, it could cause the cells to release pro-inflammatory cytokines (IL-6, TNF-α),and the ROS level in the cells increased sharply, leading to inflammatory damage [ 10 ]. Supplementing antioxidants could intervene in the damage caused by environmental pollutants to animals by improving their antioxidant functions [ 11 , 12 , 13 ], but excessive addition could also cause damage to animals. Generally, Cd can cause damage to cells through a variety of mechanisms such as promoting oxidative stress, inducing apoptosis, triggering DNA damage, interfering with oxidative phosphorylation processes in the mitochondria, and promoting inflammatory responses [ 14 ].…”