Vitamin C modulates Microcystis aeruginosa death and toxin release by induced Fenton reaction

Chen, Yuanyuan; Li, Jian; Wei, Jin; Kawan, Atufa; Wang, L.; Zhang, Xuezhen

doi:10.1016/j.jhazmat.2016.10.010

Cited by 26 publications

(8 citation statements)

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“…It can lead to oxidative stress that may cause DNA adducts, lipid damage and cell death by initiating apoptosis. [23][24][25][26] The present study showed the significantly higher levels of SF in maternal and umbilical cord blood samples in the PE groups. However, the mRNA and ferritin levels in placental samples were significantly lower compared with the controls.…”

Section: Discussionsupporting

confidence: 57%

Differential expression of markers of oxidative stress and apoptosis in relation to serum ferritin levels in patients with pre-eclampsia

et al. 2021

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Background and Objective: Pre-eclampsia (PE) is a hypertensive gestational disease appearing during second trimester of pregnancy. Free radicals are released by the placenta in this condition that may cause oxidative damage. This study was designed to determine the serum ferritin (SF) levels in maternal blood, fetal umbilical cord blood and placenta and the changes associated with oxidative stress as well as cell apoptosis to understand the pathogenesis of PE. Methods: This cross-sectional analytical study recruited 60 pregnant females with severe PE and assigned into early and late onset PE groups. Another n = 60 cases of normal pregnant females with similar gestational weeks were selected in the control group. Maternal serum and fetal umbilical cord blood ferritin levels were determined by automatic biochemical immunoassay system. Reverse transcription real-time fluorescence, Western blot and colorimetry were used to determine mRNA expression levels of ferritin and ferritin heavy chain, relative expression of ferritin and superoxide dismutase, malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) levels, respectively. Results: Mean age was 30.89 ± 5.65 and 31.79 ± 5.06 years in early and late onset PE groups respectively. Serum uric acid and creatinine levels of both PE groups were significantly higher than the normal pregnant females. SF levels were also higher in the participants of PE groups. However, the mRNA and ferritin protein levels in placental tissue were significantly lower in PE groups while comparing to controls. The cleaved caspase-3 protein, GSH-Px and MDA levels were significantly higher in both PE groups (p < 0.05). Conclusion: The alterations in factors related to oxidative stress and cell apoptosis in placental tissue may be helpful to understand the pathogenesis of PE and may provide potential biomarkers for the diagnosis of PE.

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Section: Discussionsupporting

confidence: 57%

Differential expression of markers of oxidative stress and apoptosis in relation to serum ferritin levels in patients with pre-eclampsia

et al. 2021

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“…As also shown, pretreatment with antioxidants can protect the cells against the occurrence of apoptosis, which was also shown in reductant-treated Microcystis [54]. Interestingly, at higher concentrations antioxidative compounds such as vitamin C can also induce PCD [64]. In a general perspective, the observed apoptosis-like death in Microcystis under various stress stimuli (e.g., herbicide, UV irradiation, and high pH) resulted from either direct oxidant treatment (H 2 O 2 ) or indirect generation of intracellular oxidative stress by stimuli.…”

Section: The Mechanisms Of Programmed Death In Microcystismentioning

confidence: 55%

“…These results indicated the involvement of a caspase-like enzyme in PA-induced growth inhibition of M. aeruginosa [51]. Ding [64]. Therefore, the increased activity of caspase-like appeared to be a conserved hallmark of apoptosis-like death in Microcystis.…”

Section: Caspase Assaymentioning

confidence: 79%

“…As proposed, PG-induced ROS formation was involved in the occurrence of the PCD [45]. Interestingly, Chen et al demonstrated that vitamin C (0.6 mM) triggered the occurrence of apoptosis in toxic M. aeruginosa FACHB905 via the Fenton reaction in which, vitamin C enhanced iron absorption leads to high ferrous ion levels that subsequently increase the production of ROS [64]. These findings consistently point to a link between the occurrence of apoptosis and the generation of ROS in stressed Microcystis cells.…”

Section: The Mechanisms Of Programmed Death In Microcystismentioning

confidence: 94%

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Programmed Cell Death-Like and Accompanying Release of Microcystin in Freshwater Bloom-Forming Cyanobacterium Microcystis: From Identification to Ecological Relevance

Rzymski

2019

Toxins

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Microcystis is the most common freshwater bloom-forming cyanobacterium. Its massive blooms not only adversely affect the functionality of aquatic ecosystems, but are also associated with the production of microcystins (MCs), a group of potent toxins that become a threat to public health when cell-bound MCs are significantly released from the dying Microcystis into the water column. Managing Microcystis blooms thus requires sufficient knowledge regarding both the cell death modes and the release of toxins. Recently, more and more studies have demonstrated the occurrence of programmed cell death-like (or apoptosis-like) events in laboratory and field samples of Microcystis. Apoptosis is a genetically controlled process that is essential for the development and survival of metazoa; however, it has been gradually realized to be an existing phenomenon playing important ecological roles in unicellular microorganisms. Here, we review the current progress and the existing knowledge gap regarding apoptosis-like death in Microcystis. Specifically, we focus first on the tools utilized to characterize the apoptosis-related biochemical and morphological features in Microcystis. We further outline various stressful stimuli that trigger the occurrence of apoptosis and discuss the potential mechanisms of apoptosis in Microcystis. We then propose a conceptual model to describe the functional coupling of apoptosis and MC in Microcystis. This model could be useful for understanding both roles of MC and apoptosis in this species. Lastly, we conclude the review by highlighting the current knowledge gap and considering the direction of future research. Overall, this review provides a recent update with respect to the knowledge of apoptosis in Microcystis and also offers a guide for future investigations of its ecology and survival strategies.

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“…Their content in Microcystis can be increased under the various abiotic stressors which act as direct oxidants or trigger the internal generation of ROS (Ross et al, 2006;Ding et al, 2013;Hu and Rzymski, 2019). So far, the PCD in Microcystis has been shown to be induced by exogenous oxidants (e.g., H 2 O 2 ; Ross et al, 2006;Ding et al, 2012;Zhou et al, 2018), mesohaline conditions (Ross et al, 2019), darkness (Bouchard and Purdie, 2011), high concentration of ascorbic acid (Chen et al, 2017), aldehydes (e.g., cinnamaldehyde; Hu et al, 2011), herbicides (fenoxaprop-p-ethyl, glyphosate, and methyl viologen; Ross et al, 2006;Wu et al, 2016;Du et al, 2017;Ye et al, 2019), allelochemicals (pyrogallic acid and phenolic compounds; He et al, 2016;Lu et al, 2017), bacterial pigments (e.g., prodigiosin; Yang et al, 2017), high pH, depletion of CO 2 (Sigee et al, 2007), and ultraviolet radiation (Ding et al, 2013). The present study is the first to couple ROS production and PCD in Microcystis under the influence of molecules originating from zooplankton organisms.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress, Programmed Cell Death and Microcystin Release in Microcystis aeruginosa in Response to Daphnia Grazers

et al. 2020

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There is increasing evidence that programmed cell death (PCD) in cyanobacteria is triggered by oxidative stress and that it contributes to the survival of the cyanobacterial population such as Microcystis aeruginosa. At the same time, microcystins (MCs) released during cell lysis have been implicated in colony formation (enabled by the release of polysaccharides) in M. aeruginosa-a strategy that allows the effect of a stressor, including grazing to be avoided or decreased. This experimental research has explored whether extracts of Daphnia magna and Daphnia cucullata (corresponding to 5, 25, 50, and 100 individuals per liter) reveal the effect on the growth, intracellular reactive oxygen species (ROS) content, lipid peroxidation, PCD, MC-LR release, and bound exopolysaccharide (EPS) level in M. aeruginosa during 7 days of exposure. As demonstrated, extracts of both daphnids induced dose-dependent growth inhibition, increase in ROS levels, lipid peroxidation, and PCD. Moreover, the release of MC-LR and an increase in the bound EPS fraction were observed in treated cultures. Generally, the greatest effects were observed under the influence of D. magna extracts. The study indicates that grazer presence can potentially trigger a series of events in the Microcystis population, with cells undergoing oxidative stress-induced PCD associated with MC release, which in turn increases EPS production by intact cells. As argued, this strategy is likely to have evolved in response to abiotic stressors, since both PCD and synthesis of MC in cyanobacteria predate the metazoan lineage. Nevertheless, it may still provide a benefit for the survival of the MC-producing M. aeruginosa population under grazer pressure.

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Vitamin C modulates Microcystis aeruginosa death and toxin release by induced Fenton reaction

Cited by 26 publications

References 48 publications

Differential expression of markers of oxidative stress and apoptosis in relation to serum ferritin levels in patients with pre-eclampsia

Differential expression of markers of oxidative stress and apoptosis in relation to serum ferritin levels in patients with pre-eclampsia

Programmed Cell Death-Like and Accompanying Release of Microcystin in Freshwater Bloom-Forming Cyanobacterium Microcystis: From Identification to Ecological Relevance

Oxidative Stress, Programmed Cell Death and Microcystin Release in Microcystis aeruginosa in Response to Daphnia Grazers

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