Vitamin D and melatonin protect the cell’s viability and ameliorate the CCl4 induced cytotoxicity in HepG2 and Hep3B hepatoma cell lines

Özerkan, Dilşad; Özsoy, Nesrin; Yılmaz, Erkan

doi:10.1007/s10616-014-9738-8

Cited by 20 publications

(6 citation statements)

References 45 publications

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“…Others have used these doses of CCl 4 in HepG2 and Hep3B cells for in vitro treatments. ( 19 ) Cell‐free supernatants were used to determine cytotoxicity using lactate dehydrogenase (LDH)–cytotoxicity assay kit II according to the manufacturer’s instructions (Abcam).…”

Section: Methodsmentioning

confidence: 99%

“…Because we found a higher level of miR-208a in Kupffer cells, we optimized in vitro CCl 4 treatment in RAW 264.7 macrophages using the same concentrations as previously described for hepatocyte cell lines. (19) RAW macrophages contain cytochrome p450 genes for metabolism of drugs and organic compounds. (32) In vitro CCl 4 treatment for 6 hours at the concentration of 0.2%, 0.3%, and 0.4% caused significant cytotoxicity in RAW macrophages (Fig.…”

Section: Mir-208a Overexpression Attenuates CCL 4 -Induced Cytotoxicimentioning

confidence: 99%

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Deficiency of miR‐208a Exacerbates CCl4‐Induced Acute Liver Injury in Mice by Activating Cell Death Pathways

et al. 2020

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Acute liver injury (ALI) is associated with multiple cellular events such as necrosis, apoptosis, oxidative stress and inflammation, which can lead to liver failure. In this study, we demonstrate a new role of microRNA (miR)-208a in ALI. ALI was induced in wild-type (WT) and miR-208a knockout (KO) mice by CCl 4 administration. Increased alanine aminotransferase and decreased hepatic miR-208a levels were found in WT mice after acute CCl 4 treatment. Histopathological evaluations revealed increased necrosis and decreased inflammation in miR-208a KO compared with WT mice after CCl 4 treatment. CCl 4 treatment induced a higher alanine aminotransferase elevation and increased numbers of circulating extracellular vesicles (exosomes and microvesicles) in miR-208a KO compared with WT mice. We found increased CCl 4-induced nuclear factor kappa B activation and tumor necrosis factor-α induction and decreased monocyte chemoattractant protein 1 levels in miR-208a KO compared with WT mice. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling assay indicated aggravated hepatic apoptosis and necrosis in CCl 4-treated miR-208a KO compared with WT mice. CCl 4 treatment induced a greater increase in cleaved caspase-8, p18, and caspase-3 in miR-208a KO compared with WT mice. p53 is involved in various cell death pathways, including necrosis and apoptosis. Our in silico analysis revealed p53 as a predicted miR-208a target, and we found enhanced p53 and cyclophilin D protein expressions in miR-208a KO mice after CCl 4 treatment. Increased liver injury in miR-208a KO mice was further associated with increased Bax (B cell lymphoma 2-associated X protein) and p21 expression. Our in vitro results indicated a role of miR-208a in cell death. We found that CCl 4-induced cytotoxicity was partially rescued by miR-208a overexpression in RAW macrophages. Altogether, our results revealed a role of miR-208a in ALI in mice and suggest a role for miR-208a in regulating cell death. (Hepatology Communications 2020;4:1487-1501). A cute liver injury (ALI) is the leading cause of liver failure. (1) Oxidative stress, necrosis, apoptosis, and inflammation are the main events of ALI, which in some cases can lead to the liver failure. Acute liver failure (ALF) can be caused by various etiologies including drugs, viral hepatitis, and ischemia. (1) ALF is a life-threatening condition and has a high mortality rate. (1) Liver transplantation is a life-saving therapy in severe ALF cases. The ALI model of CCl 4 has been used widely in the hepatology field, as it induces oxidative stress, apoptosis, necrosis, inflammation, and fibrosis in mice.

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Section: Methodsmentioning

confidence: 99%

Section: Mir-208a Overexpression Attenuates CCL 4 -Induced Cytotoxicimentioning

confidence: 99%

Deficiency of miR‐208a Exacerbates CCl4‐Induced Acute Liver Injury in Mice by Activating Cell Death Pathways

et al. 2020

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“…The viability of cells was then determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. 17,18…”

Section: In Vitro Hepatoprotective Activity Against CCL 4 Induced Toxicity On Hepg2 Cellsmentioning

confidence: 99%

Antioxidant and Hepatoprotective Activity of Phenyl Glycosides Isolated From Heliciopsis lobata

Trung

Thảo

Anh

et al. 2020

Natural Product Communications

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A new macrocyclic glycoside named helilobatoside A (1) and 5 known phenyl glycosides as 3,5-dimethoxy-4-hydroxyphenyl-1- O-β-d-glucopyranoside (2), tachioside (3), isotachioside (4), 1-(4-hydroxy-3-methoxyphenyl)-1-propanone-3- O-β-d-glucopyranoside (5), and 1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone-3- O-β-d-glucopyranoside (6), were isolated from the wood of Heliciopsis lobata (Merr.) Sleumer. Their chemical structures were elucidated using a combination of high-resolution electrospray ionization mass spectrometry, 1-dimensional (1D) and 2D nuclear magnetic resonance (NMR) spectral data as well as by comparison with data in the previous literature. This is the first time the 13C NMR data of compounds 5 and 6 were reported and also were assigned by heteronuclear single quantum correlation and heteronuclear multiple bond correlation spectra. Compounds 2-6 were first isolated from Heliciopsis genus. The isolated compounds were evaluated for their antioxidant and hepatoprotective activities in vitro. Compound 2 showed potential as an antioxidant in a 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay (half-maximal inhibitory concentration [IC50] = 6.07 ± 0.17 µg/mL) and in thio-barbituric acid reactive substances assay (IC50 = 89.55 ± 8.26 µg/mL). This compound could also reduce the toxic effects of carbon tetrachloride on HepG2 survival and significantly protect the viability of cells up to 52.25 ± 4.36% at the 100 µg/mL treatment ( P < 0.05). Thus, with obtained results, the hepatoprotective activity of compound 2 could be related to radical scavenging and limited the lipid peroxidative activities.

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“…Kết quả thí nghiệm trên cho thấy, tỷ lệ phần trăm tế bào sống ở nghiệm thức tế bào được nuôi cấy trong môi trường DMEM có DMSO 0,25% có sự khác biệt không có ý nghĩa so với nghiệm thức đối chứng môi trường bình thường DMEM. Kết quả này cho thấy DMSO ở nồng độ 0,25% không có độc tính đối với dòng tế bào HepG2 phù hợp với kết quả nghiên cứu của Ozerkan [23], nên có thể sử dụng dung môi DMSO để pha các cao chiết methanol.…”

Section: Trang 18unclassified

Study on the antioxidant and anti-cancer activities in HEPG2 cells of Ixora duffii

Phan

Dai

2018

Sci. Tech. Dev. J. - Nat. Sci.

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Ixora duffii is an important traditional medicinal plant in Vietnam. In this study its antioxidant property was investigated by diphenylpicrylhydrazyl (DPPH) radical and Fe3+ reducing assay and cytotoxic activity was evaluated against HepG2 cell line by MTT assay. The results showed that the methanolic extract of Ixora duffii flowers had DPPH radical scavenging activities similar to that of leaves extract. At the concentration of 100 􀁐g/mL, the extracts of flowers and leaves scavenged about 80% DPPH radical. The DPPH scavenging activity of Ixora duffii was lower than that of vitamin C approximately 2 times. The activity of Fe3+ reducing of flowers was higher than that of leaves, with EC50 values of 162.03 and 218.87 􀁐g/mL, respectively. Results were compared with the standard butylated hydroxyanisole (BHA) that was lower than 4.78 times in flowers and 6.76 times in leaves. The methanolic extract of Ixora duffii displayed significantly dose dependent in reducing the growth of HepG2, with 56% growth inhibitory concentration in a dose of 500 􀁐g/mL. The qualitative analysis of phytochemical compounds showed the presence of alkaloids, flavonoids, anthraquinones, terpenoids, quinones, glycoside, coumarins and phenols in the leaves and flower extracts of Ixora duffii. Compounds of tanins and saponins were only present in flowers of Ixora duffii. Total phenolic content were found in flowers (762.37 mg GAE/g) that was higher than the one in leaves (360.85 mg GAE/g). Flower and leave extracts exhibited a similar total flavonoid content of 679.55 mg QE/g and 676.35 mg QE/g, respectively.

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Vitamin D and melatonin protect the cell’s viability and ameliorate the CCl4 induced cytotoxicity in HepG2 and Hep3B hepatoma cell lines

Cited by 20 publications

References 45 publications

Deficiency of miR‐208a Exacerbates CCl4‐Induced Acute Liver Injury in Mice by Activating Cell Death Pathways

Deficiency of miR‐208a Exacerbates CCl4‐Induced Acute Liver Injury in Mice by Activating Cell Death Pathways

Antioxidant and Hepatoprotective Activity of Phenyl Glycosides Isolated From Heliciopsis lobata

Study on the antioxidant and anti-cancer activities in HEPG2 cells of Ixora duffii

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