Vitamin D Deficiency Induces Elevated Oxidative and Biomechanical Damage in Coronary Arterioles in Male Rats

Sziva, Réka Eszter; Fontányi, Zoltán; Pál, Éva; Hadjadj, Leila; Monori-Kiss, Anna; Horváth, Eszter; Benkó, Rita; Magyar, Attila; Heinzlmann, Andrea; Benyó, Zoltán; Nádasy, György L.; Várbı́ró, Szabolcs

doi:10.3390/antiox9100997

Cited by 9 publications

(13 citation statements)

References 55 publications

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“…The average 25-OH-D 3 levels in the experimental groups were the following: FD+: 32.328 ± 4.49 ng/mL; FD–: 6.044 ± 0.63 ng/mL; MD+: 19.66 ± 0.81 ng/mL; MD– rats: 3.59 ± 0.21 ng/mL [ 7 , 9 ]. All animals were normotensive [ 9 , 10 ]. Vitamin D status had no significant effect on final body weight, weight gain, serum progesterone or testosterone levels in any genders [ 7 , 9 , 10 ].…”

Section: Methodsmentioning

confidence: 99%

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Vitamin D Deficiency Cause Gender Specific Alterations of Renal Arterial Function in a Rodent Model

et al. 2021

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Vitamin D deficiency shows positive correlation to cardiovascular risk, which might be influenced by gender specific features. Our goal was to examine the effect of Vitamin D supplementation and Vitamin D deficiency in male and female rats on an important hypertension target organ, the renal artery. Female and male Wistar rats were fed with Vitamin D reduced chow for eight weeks to induce hypovitaminosis. Another group of animals received normal chow with further supplementation to reach optimal serum vitamin levels. Isolated renal arteries of Vitamin D deficient female rats showed increased phenylephrine-induced contraction. In all experimental groups, both indomethacin and selective cyclooxygenase-2 inhibition (NS398) decreased the phenylephrine-induced contraction. Angiotensin II-induced contraction was pronounced in Vitamin D supplemented males. In both Vitamin D deficient groups, acetylcholine-induced relaxation was impaired. In the female Vitamin D supplemented group NS398, in males the indomethacin caused reduced acetylcholine-induced relaxation. Increased elastic fiber density was observed in Vitamin D deficient females. The intensity of eNOS immunostaining was decreased in Vitamin D deficient females. The density of AT1R staining was the highest in the male Vitamin D deficient group. Although Vitamin D deficiency induced renal vascular dysfunction in both sexes, female rats developed more extensive impairment that was accompanied by enzymatic and structural changes.

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Section: Methodsmentioning

confidence: 99%

“…All animals were normotensive [ 9 , 10 ]. Vitamin D status had no significant effect on final body weight, weight gain, serum progesterone or testosterone levels in any genders [ 7 , 9 , 10 ].…”

Section: Methodsmentioning

confidence: 99%

Vitamin D Deficiency Cause Gender Specific Alterations of Renal Arterial Function in a Rodent Model

et al. 2021

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“…As our workgroup published previously, physiological, hormonal, and glucose-metabolism parameters; systolic and diastolic blood pressures; and the body weight of the rats measured did not differ between the two groups at the end of the experiment [23,25]. There was no significant difference between heart weights either (in Mean ± SEM: 1.41 ± 0.051 g and 1.55 ± 0.048 g for the control and VDD groups, respectively, n.s.…”

Section: Heart Weightmentioning

confidence: 55%

Vitamin D Deficiency Reduces Vascular Reactivity of Coronary Arterioles in Male Rats

Fontányi

Sziva

Pál

et al. 2021

CIMB

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Background: Vitamin D deficiency (VDD) may be considered an independent cardiovascular (CV) risk factor, and it is well known that CV risk is higher in males. Our goal was to investigate the pharmacological reactivity and receptor expression of intramural coronary artery segments of male rats in cases of different vitamin D supply. Methods: Four-week-old male Wistar rats were divided into a control group (n = 11) with optimal vitamin D supply (300 IU/kgbw/day) and a VDD group (n = 11, <0.5 IU/kgbw/day). After 8 weeks of treatment, intramural coronary artery segments were microprepared, their pharmacological reactivity was examined by in vitro microangiometry, and their receptor expression was investigated by immunohistochemistry. Results: Thromboxane A2 (TXA2)-agonist induced reduced vasoconstriction, testosterone (T) and 17-β-estradiol (E2) relaxations were significantly decreased, a significant decrease in thromboxane receptor (TP) expression was shown, and the reduction in estrogen receptor-α (ERα) expression was on the border of significance in the VDD group. Conclusions: VD-deficient male coronary arteries showed deteriorated pharmacological reactivity to TXA2 and sexual steroids (E2, T). Insufficient vasoconstrictor capacity was accompanied by decreased TP receptor expression, and vasodilator impairments were mainly functional. The decrease in vasoconstrictor and vasodilator responses results in narrowed adaptational range of coronaries, causing inadequate coronary perfusion that might contribute to the increased CV risk in VDD.

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“…Rats were housed at constant room temperature (22 ± 1 °C) with a 12 h/12 h light–dark cycle. All experimental groups had normal blood pressure [ 45 , 46 ]. Serum 25(OH)D levels of the animals were the following: FD+: 32.328 ± 4.49 ng/mL; FD−: 6.044 ± 0.63 ng/mL; MD+: 19.66 ± 0.81 ng/mL; MD− rats: 3.59 ± 0.21 ng/mL [ 43 , 45 ].…”

Section: Methodsmentioning

confidence: 99%

“…Serum 25(OH)D levels of the animals were the following: FD+: 32.328 ± 4.49 ng/mL; FD−: 6.044 ± 0.63 ng/mL; MD+: 19.66 ± 0.81 ng/mL; MD− rats: 3.59 ± 0.21 ng/mL [ 43 , 45 ]. Final body weight, weight gain and serum testosterone levels were not significantly influenced by vitamin D status in either gender [ 43 , 45 , 46 ].…”

Section: Methodsmentioning

confidence: 99%

Vitamin D Deficiency and Gender Alter Vasoconstrictor and Vasodilator Reactivity in Rat Carotid Artery

Sipos

Gerszi

Dalloul

et al. 2021

IJMS

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The vitamin-D-sensitivity of the cardiovascular system may show gender differences. The prevalence of vitamin D (VD) deficiency (VDD) is high, and it alters cardiovascular function and increases the risk of stroke. Our aim was to investigate the vascular reactivity and histological changes of isolated carotid artery of female and male rats in response to different VD supplies. A total of 48 male and female Wistar rats were divided into four groups: female VD supplemented, female VDD, male VD supplemented, male VDD. The vascular function of isolated carotid artery segments was examined by wire myography. Both vitamin D deficiency and male gender resulted in increased phenylephrine-induced contraction. Acetylcholine-induced relaxation decreased in male rats independently from VD status. Inhibition of prostanoid signaling by indomethacin reduced contraction in females, but increased relaxation ability in male rats. Functional changes were accompanied by VDD and gender-specific histological alterations. Elastic fiber density was significantly decreased by VDD in female rats, but not in males. Smooth muscle actin and endothelial nitric oxide synthase levels were significantly lowered, but the thromboxane receptor was elevated in VDD males. Decreased nitrative stress was detected in both male groups independently from VD supply. The observed interactions between vitamin D deficiency and sex may play a role in the gender difference of cardiovascular risk.

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Vitamin D Deficiency Induces Elevated Oxidative and Biomechanical Damage in Coronary Arterioles in Male Rats

Cited by 9 publications

References 55 publications

Vitamin D Deficiency Cause Gender Specific Alterations of Renal Arterial Function in a Rodent Model

Vitamin D Deficiency Cause Gender Specific Alterations of Renal Arterial Function in a Rodent Model

Vitamin D Deficiency Reduces Vascular Reactivity of Coronary Arterioles in Male Rats

Vitamin D Deficiency and Gender Alter Vasoconstrictor and Vasodilator Reactivity in Rat Carotid Artery

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