Vitamin D maintains E-cadherin intercellular junctions by downregulating MMP-9 production in human gingival keratinocytes treated by TNF-α

Oh, Changseok; Kim, Hyun Jung

doi:10.5051/jpis.2019.49.5.270

Cited by 29 publications

(26 citation statements)

References 39 publications

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“…Confirming the data of Oh et al [51], which showed that vitamin D downregulates MMP9 production in the presence of hypovitaminosis by blocking NFkB pathway activation, the increase of MMP9 in our PD patients is plausible. In relation to disease stage, MMP9 followed an increase (p < 0.05) in the presence of increased periodontal pocket depth, favoring tooth loss and the establishment of chronic inflammation [52].…”

Section: Discussionsupporting

confidence: 92%

Evaluation of Salivary Cytokines and Vitamin D Levels in Periodontopathic Patients

Costantini

Sinjari

Piscopo

et al. 2020

IJMS

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Periodontal disease (PD) is an inflammatory condition of the tissues supporting the teeth, which is widespread among the adult population. Evidence shows a relationship between PD and vitamin D levels, which is involved in the regulation of bone metabolism, mineral homeostasis, and inflammatory response. This study aimed to perform a simultaneous evaluation of inflammatory mediators and vitamin D levels in saliva in periodontopathic patients to better understand their role in periodontal disease. In this observational study, clinical periodontal parameter examination was performed for each patient. Moreover, the saliva levels of 25(OH)D3, TGFβ, IL-35, IL-17A, and MMP9 were evaluated using an ELISA assay. An increase in TGFβ, IL-35, MMP9, and IL-17A salivary levels and a reduction in 25(OH)D3 levels were observed in periodontopathic patients with respect to the healthy controls. The present study revealed significant positive correlation between cytokines and highly negative correlation between 25(OH)D3 and salivary cytokine levels. Further studies are needed to better understand if salivary cytokines and vitamin D evaluation may represent a new approach for detection and prevention of progressive diseases, such as PD.

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Section: Discussionsupporting

confidence: 92%

Evaluation of Salivary Cytokines and Vitamin D Levels in Periodontopathic Patients

Costantini

Sinjari

Piscopo

et al. 2020

IJMS

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“…Others supported the idea that patients with higher levels of vitamin D were related to less bleeding on probing (BoP) comparing to patients with lower levels [ 34 ]. In addition, in vitro studies demonstrated that vitamin D may decrease the number of Porphyromonas gingivalis through active autophagy [ 37 ] and might decrease the inflammatory burden of periodontitis in rodent models [ 38 , 39 , 40 , 41 ]. Furthermore, the association between vitamin D levels and periodontitis has been systematically investigated [ 42 , 43 , 44 , 45 ]; however, none of these studies were able to produce quantitative synthesis.…”

Section: Introductionmentioning

confidence: 99%

Vitamin D and Periodontitis: A Systematic Review and Meta-Analysis

Machado

Lobo

Proença³

et al. 2020

Nutrients

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To explore the vitamin D levels of periodontitis patients in comparison with periodontally healthy ones, and to assess the influence of vitamin D supplementation as an adjunctive during nonsurgical periodontal treatment (NSPT). Five databases (Pubmed, Embase, Scholar, Web of Sciences, and Cochrane Library) were searched until May 2020. Mean difference (MD) meta-analysis with corresponding 95% confidence interval (95% CI) and sensitivity tests via meta-regression were used. We followed Strength of Recommendation Taxonomy (SORT) to appraise the strength and quality of the evidence. Sixteen articles were included, fourteen case-control and two intervention studies, all reporting 25-hydroxyvitamin D (25(OH)D) levels. Compared with the healthy controls, the circulating 25(OH)D levels were significantly lower in chronic periodontitis patients (pooled MD = −6.80, 95% CI: −10.59 to −3.02). Subgroup analysis revealed differences among 25(OH)D measurements, with liquid chromatography-mass spectrometry being the most homogeneous method (pooled MD = −2.05, 95% CI: −3.40 to −0.71). Salivary levels of 25(OH)D showed no differences between groups. Due to the low number of studies, conclusions on aggressive periodontitis and in the effect of vitamin D supplementation after NSPT were not possible to ascribe. Compared with healthy controls, 25(OH)D serum levels are significantly lower in chronic periodontitis patients, with an overall SORT A recommendation. Future studies are needed to clarify the effect of vitamin D supplementation and the biological mechanisms linking vitamin D to the periodontium.

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“…In human bronchial epithelial cells (A549), vitamin D increased the expression of E-cadherin and thereby induced differentiation [ 31 ]. In gingival keratinocytes, vitamin D improved intercellular junctions by increasing E-cadherin expression [ 32 ]. The loss of VDR was associated with reduced expression of E-cadherin and epithelial cell differentiation in chronic kidney disease [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…Primary bronchial epithelial were isolated and characterized as described earlier [ 32 ]. Endo-bronchial tissue biopsies were obtained from six patients by endo-bronchoscopy for diagnostic reasons by the lung clinic (University Hospital Basel, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

Up-Regulated Vitamin D Receptor by Pelargonium sidoides Extract EPs® 7630 Contributes to Rhinovirus Defense in Bronchial Epithelial Cells

2021

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EPs®7630, extracted from Pelargonium sidoides, reduces the severity of viral upper respiratory tract infections. Vitamin D also improves anti-viral host defense through similar signaling pathways. This study assessed if EPs®7630 modifies vitamin D receptor (VDR) expression and function by human bronchial epithelial cells. Bronchial epithelial cells were incubated with EPs®7630 over 48 h before calcitriol stimulation and/or infection with Rhinovirus (RV)-16. Protein expression was determined by Western-blotting. Intracellular signaling of mitogen activated protein kinases (MAPK) was studied by chemical inhibitors. The anti-viral effect was assessed by immunofluorescence for RV-16 protein. EPs®7630 upregulated VDR expression through Erk1/2 MAPK and thereby increased the cell’s sensitivity to calcitriol. Compared ton untreated cells, the shift of the VDR into the nucleus at 5.3 times lower calcitriol concentration. EPs®7630 increased Erk1/2 MAPK signaling, but reduced p38 phosphorylation, and had no effect on Jun N-terminal kinase (JNK). EPs®7630 improved the anti-viral effect of vitamin D on RV-16 infection by 2.1 folds compared to vitamin D alone or to untreated cells. Furthermore, EPs®7630 improved the differentiation of epithelial cells by upregulating E-cadherin expression through Erk1/2. In conclusion, EPs®7630 increased host defense against Rhinovirus infection by upregulating the VDR and the differentiation of epithelial cells.

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Vitamin D maintains E-cadherin intercellular junctions by downregulating MMP-9 production in human gingival keratinocytes treated by TNF-α

Cited by 29 publications

References 39 publications

Evaluation of Salivary Cytokines and Vitamin D Levels in Periodontopathic Patients

Evaluation of Salivary Cytokines and Vitamin D Levels in Periodontopathic Patients

Vitamin D and Periodontitis: A Systematic Review and Meta-Analysis

Up-Regulated Vitamin D Receptor by Pelargonium sidoides Extract EPs® 7630 Contributes to Rhinovirus Defense in Bronchial Epithelial Cells

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