Vitamin D Promotes Odontogenic Differentiation of Human Dental Pulp Cells via ERK Activation

Woo, Su-Mi; Lim, Hyo Soon; Jeong, Kyung-Yi; Kim, Seon-Mi; Kim, Wonjae; Jung, Ji‐Yeon

doi:10.14348/molcells.2015.2318

Cited by 31 publications

(30 citation statements)

References 30 publications

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“…Cell migration (chemotaxis) was also evaluated by using a Boyden chamber (membrane pore size = 8 µm) (Cytoselect ® , Cell Biolabs, San Diego, CA, USA) 65 . Rat primary pulp cells were seeded into the upper chamber in α-MEM containing 1% (v/v) FBS at a density of 2.0 × 10 4 cells, and MMP-treated DMCs or incubated DMCs without MMP (0.01–1.0 µg/ml) as an experimental control in α-MEM containing 1% FBS were added to the lower chamber.…”

Section: Methodsmentioning

confidence: 99%

Dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases

Okamoto

Takahashi

Komichi

et al. 2018

Sci Rep

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Dentin is primarily composed of hydroxyapatite crystals within a rich organic matrix. The organic matrix comprises collagenous structural components, within which a variety of bioactive molecules are sequestered. During caries progression, dentin is degraded by acids and enzymes derived from various sources, which can release bioactive molecules with potential reparative activity towards the dentin-pulp complex. While these molecules’ repair activities in other tissues are already known, their biological effects are unclear in relation to degradation events during disease in the dentin-pulp complex. This study was undertaken to investigate the effects of dentin matrix components (DMCs) that are partially digested by matrix metalloproteinases (MMPs) in vitro and in vivo during wound healing of the dentin-pulp complex. DMCs were initially isolated from healthy dentin and treated with recombinant MMPs. Subsequently, their effects on the behaviour of primary pulp cells were investigated in vitro and in vivo. Digested DMCs modulated a range of pulp cell functions in vitro. In addition, DMCs partially digested with MMP-20 stimulated tertiary dentin formation in vivo, which exhibited a more regular tubular structure than that induced by treatment with other MMPs. Our results indicate that MMP-20 may be especially effective in stimulating wound healing of the dentin-pulp complex.

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Section: Methodsmentioning

confidence: 99%

Dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases

Okamoto

Takahashi

Komichi

et al. 2018

Sci Rep

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“…The process of dentinal regeneration involves the proliferation and differentiation of DPCs into odontoblasts, dental pulp healing and reparative dentin formation (27). A high sucrose diet affects the dentin-pulp complex and reduces dentin formation, which contributes to caries in dentin (28).…”

Section: Discussionmentioning

confidence: 99%

Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions

Lü

Sun

Liu

2017

International Journal of Molecular Medicine

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Insulin-like growth factor-1 (IGF-1) promotes human dental pulp stem cell proliferation and osteogenic differentiation. However, the effects of IGF-1 on the proliferation, apoptosis and odontoblastic differentiation (mineralization) of dental pulp cells (DPCs) under high glucose (GLU) conditions remain unclear. In this study, isolated primary human DPCs were treated with various concentrations of high GLU. Cell proliferation and apoptosis were determined by Cell Counting Kit-8 and Annexin V-FITC/PI assays, respectively. The cells were cultured in odontoblastic induction medium containing various concentrations of high GLU. Odontoblastic differentiation was determined by alkaline phosphatase (ALP) activity assay. Mineralization formation was evaluated by von Kossa staining. The expression levels of IGF family members were measured by western blot analysis and RT-qPCR during proliferation and differentiation. The cells were then exposed to 25 mM GLU and various concentrations of IGF-1. Cell proliferation, apoptosis, ALP activity, mineralization formation and the levels of mineralization-related proteins were then evaluated. Our results revealed that high GLU significantly inhibited cell proliferation and promoted cell apoptosis. GLU (25 and 50 mM) markedly reduced ALP activity and mineralization on days 7 and 14 after differentiation. The levels of IGF family members were markedly decreased by high GLU during proliferation and differentiation. However, IGF-1 significantly reversed the effects of high GLU on cell proliferation and apoptosis. Additionally, IGF-1 markedly restored the reduction of ALP activity and mineralization induced by high GLU. Our findings thus indicate that IGF-1 attenuates the high GLU-induced inhibition of DPC proliferation, differentiation and mineralization.

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“…These data may thus help to decipher the physiological endocrine-mediated regulations of amelogenesis and enamel pathologies resulting from endocrine disruption. To date, only the vitamin D pathway has been investigated in dental cells (Berdal et al, 1993; Descroix et al, 2010; Woo et al, 2015). Dental endocrinology needs to be explored in depth to understand the pathways of hormones effects on dental growth and enamel quality.…”

Section: Introductionmentioning

confidence: 99%

Expression of Steroid Receptors in Ameloblasts during Amelogenesis in Rat Incisors

et al. 2016

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Endocrine disrupting chemicals (EDCs) play a part in the modern burst of diseases and interfere with the steroid hormone axis. Bisphenol A (BPA), one of the most active and widely used EDCs, affects ameloblast functions, leading to an enamel hypomineralization pattern similar to that of Molar Incisor Hypomineralization (MIH). In order to explore the molecular pathways stimulated by BPA during amelogenesis, we thoroughly investigated the receptors known to directly or indirectly mediate the effects of BPA. The expression patterns of high affinity BPA receptors (ERRγ, GPR30), of ketosteroid receptors (ERs, AR, PGR, GR, MR), of the retinoid receptor RXRα, and PPARγ were established using RT-qPCR analysis of RNAs extracted from microdissected enamel organ of adult rats. Their expression was dependent on the stage of ameloblast differentiation, except that of ERβ and PPARγ which remained undetectable. An additional large scale microarray analysis revealed three main groups of receptors according to their level of expression in maturation-stage ameloblasts. The expression level of RXRα was the highest, similar to the vitamin D receptor (VDR), whereas the others were 13 to 612-fold lower, with AR and GR being intermediate. Immunofluorescent analysis of VDR, ERα and AR confirmed their presence mainly in maturation- stage ameloblasts. These data provide further evidence that ameloblasts express a specific combination of hormonal receptors depending on their developmental stage. This study represents the first step toward understanding dental endocrinology as well as some of the effects of EDCs on the pathophysiology of amelogenesis.

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Vitamin D Promotes Odontogenic Differentiation of Human Dental Pulp Cells via ERK Activation

Cited by 31 publications

References 30 publications

Dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases

Dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases

Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions

Expression of Steroid Receptors in Ameloblasts during Amelogenesis in Rat Incisors

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