Vitamin D Receptor Gene Expression in Adipose Tissue of Obese Individuals is Regulated by miRNA and Correlates with the Pro-Inflammatory Cytokine Level

Jonas, Marta; Kuryłowicz, Alina; Bartoszewicz, Zbigniew; Lisik, Wojciech; Jonas, Maurycy; Koźniewski, Krzysztof; Puzianowska-Kuźnicka, Monika

doi:10.3390/ijms20215272

Cited by 32 publications

(31 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our finding that the gene for the Vitamin D receptor was the most up-regulated gene in ciliated cells in response to MCH was at first surprising to us, but upon further examination and when placed in the context of the complete transcriptome response in ciliated adipocytes, it becomes clearer. Vitamin D regulates the inflammatory response in adipocytes 36 , and modulation of receptor availability by MCH could be a means by which the inflammatory response is controlled. Expression of vdr is elevated in early adipogenesis and it is hypothesized that 1,25(OH)2D3 could exert an anti-adipogenic effect via Wnt/b-catenin.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome analysis of ciliary-dependent MCH signaling in differentiating 3T3-L1 pre-adipocytes

Cook

Ophardt

Shen

et al. 2021

Sci Rep

View full text Add to dashboard Cite

An understanding of adipocyte responsiveness to G-protein-coupled receptor-(GPCR) derived signals must take into consideration the role of membrane microenvironments; that individual sub-populations of proteins may vary significantly across different regions of the cell, and that cell differentiation alters those microenvironments. 3T3-L1 pre-adipocytes undergo a dramatic phenotypic transformation during differentiation into adipocytes, requiring the development of a transient primary cilium. We demonstrate that melanin-concentrating hormone (MCH) receptor 1, a GPCR that stimulates appetite, translocates to the transient primary cilium during early 3T3-L1 cell adipogenesis. Furthermore, we used RNA-Seq to investigate whether MCH signaling is influenced by its receptor localization and whether MCH can influence the transcriptome of early adipocyte development. We found that MCH signaling is sensitive to receptor localization to cilia, and this alters the adipogenic transcriptional program. Also, novel MCH signaling pathways in 3T3-L1 cells are identified, including those for circadian rhythm, the inflammatory response, and ciliary biogenesis. The presence of active MCH-signaling pathways in pre-adipocytes and the discovery that these pathways intersect with the early adipogenic program, among other newly-identified signaling pathways, suggests that the use of MCH receptor 1 antagonists for clinical interventions may have unintended consequences on adipose tissue development.

show abstract

Section: Resultsmentioning

confidence: 99%

Transcriptome analysis of ciliary-dependent MCH signaling in differentiating 3T3-L1 pre-adipocytes

Cook

Ophardt

Shen

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Moreover the above mechanisms would not explain why the differential release is only observed for 1,25(OH) 2 D 3 and not for 25(OH)D 3. Vitamin D 1,25(OH) 2 D 3 is a ligand for vitamin D receptor (VDR), and it has been shown that vitamin D receptor (VDR) expression is increased in SAT of individuals with obesity (Jonas et al, 2019). From the latter, it could be speculated that vitamin D 1,25(OH) 2 D 3 binds to a higher extent to VDR within SAT in individuals with obesity, resulting in less spillover of vitamin D 1,25(OH) 2 D 3 in the circulation in obese individuals but not in lean, which still needs further investigation.…”

Section: Discussionmentioning

confidence: 99%

Vitamin D release across abdominal adipose tissue in lean and obese men: The effect of ß‐adrenergic stimulation

et al. 2019

View full text Add to dashboard Cite

Obesity is characterized by a blunted lipolytic response in abdominal subcutaneous adipose tissue (SAT) and low circulating vitamin D levels. Here we investigated whether an impaired SAT lipolytic response coincides with an impaired SAT vitamin D release in eight lean and six obese men. 25‐hydroxyvitamin D3 [25(OH)D3] and 1,25‐dihydroxyvitamin D3 [1,25(OH)2D3] fluxes across SAT were measured using arterio‐venous blood sampling in combination with AT blood flow measurements after an overnight fast and during 1‐hr intravenous infusion of the non‐selective ß‐adrenergic agonist isoprenaline (20 ng·kg FFM−1·min−1). 1,25(OH)2D3 was released across abdominal SAT during isoprenaline infusion in lean [−0.01 (−0.04 to 0.00) pmol*100 g tissue−1*min−1, p = .017 vs. zero flux], but not in obese men [0.01 (0.00 to 0.02) pmol*100 g tissue−1*min−1, p = .116 vs. zero flux], and accompanied by an impaired isoprenaline‐induced lipolytic response in abdominal SAT of obese versus lean men. Isoprenaline had no significant effects on net 25(OH)D3 release across abdominal SAT and plasma vitamin D metabolites in lean and obese men. To conclude, a blunted isoprenaline‐mediated lipolysis is accompanied by reduced release of 1,25(OH)2D3 vitamin D across abdominal SAT in obesity.

show abstract

“…Регуляторные сети микроРНК особенно важны для сигнальных молекул, к которым относится витамин D, оказывающих плейотропное действие на различные органы и ткани организма. В недавних исследованиях показана роль 1,25(OH)D в эпигенетической регуляции генов, особенно в качестве модулятора функции микроРНК [36].…”

Section: эпигенетические механизмы регуляции рецептора ви тамина Dunclassified

Genetic and epigenetic factors regulating the expression and function of the vitamin D receptor in patients with coronary artery disease

2021

View full text Add to dashboard Cite

Coronary artery disease (CAD) remains the leading cause of death and disability in developed countries. Using traditional risk factors for CAD, it is possible to predict the likelihood of acute coronary events in no more than 50% of cases. Therefore, the study of influence of genetic and epigenetic factors on the development of CAD is extremely important. Research in recent years has shown that vitamin D deficiency is a new risk factor for atherosclerosis and immune inflammation. Vitamin D implements protective effects against immune inflammation through receptors in the vascular wall. A single nucleotide polymorphism of the vitamin D receptor (VDR) gene is a potential risk factor for CAD associated with low vitamin D levels. VDR expression correlates with the expression of pro-inflammatory cytokines and is regulated by microRNAs — microRNA-125a-5p, microRNA-125b-5p, microRNA-214-3p and microRNA-21 These microRNAs regulate the action, synthesis and metabolism of vitamin D and can themselves be influenced by VDR signals through dynamic feedback, which can lead to destabilization of mRNA and inhibition of translation. This literature review highlights the effect of a single nucleotide polymorphism of the VDR gene and microRNA on the pathogenetic mechanisms of CAD.

show abstract

Vitamin D Receptor Gene Expression in Adipose Tissue of Obese Individuals is Regulated by miRNA and Correlates with the Pro-Inflammatory Cytokine Level

Cited by 32 publications

References 36 publications

Transcriptome analysis of ciliary-dependent MCH signaling in differentiating 3T3-L1 pre-adipocytes

Transcriptome analysis of ciliary-dependent MCH signaling in differentiating 3T3-L1 pre-adipocytes

Vitamin D release across abdominal adipose tissue in lean and obese men: The effect of ß‐adrenergic stimulation

Genetic and epigenetic factors regulating the expression and function of the vitamin D receptor in patients with coronary artery disease

Contact Info

Product

Resources

About