Background: Although vitamin D levels and underlying vitamin D receptor (VDR) genetic polymorphisms have been linked to many common diseases including obesity, the association remains unclear. We aimed to determine the genotypes & allele percentage frequency distribution of four polymorphisms FokI, BsmI, ApaI and TaqI in the VDR gene in healthy Emirati individuals and their association with vitamin D levels and chronic conditions including diabetes mellitus, hypertension and obesity Methods: 277 participants who were part of a randomized controlled trial had their assessment that included clinical and anthropometric data. Whole blood samples were taken for measurements of vitamin D [25(OH) D], four vitamin D receptors gene polymorphism SNPs including BsmI, FokI, TaqI and ApaI, metabolic and inflammatory markers and related biochemical variables. Multiple logistic regression analysis used to assess the influence of vitamin D receptors gene SNPs on vitamin D status after adjusting for clinical parameters known to influence vitamin status in the study population. Results: Overall, 277 participants with a mean (±SD) age of 41±12 and 204 (74%) female were included in the study. There were statistically significant differences in vitamin concentrations between different genotypes of the 4 VDR gene polymorphisms (p<0.05). There were however no statistically significant differences in vitamin concentrations between subjects with and those without the 4 VDR gene polymorphisms genotype and alleles except for AA and AG and allele G in Apal SNP (p<0.05). Multivariate analysis revealed no significant independent associations between vitamin D status and the 4 VDR gene polymorphisms after adjusting for dietary intakes, physical activity, sun exposure, smoking and body mass index. In addition, no significant differences found in the frequency of the genotypes & alleles of the 4 VDR genes among patients with obesity, diabetes and hypertension compared to those without these medical conditions. Conclusion: Although we found statistically significant differences in vitamin concentrations between different genotypes of the 4 VDR gene polymorphisms, however multivariate analysis revealed no association after adjusting for clinical parameters known to influence vitamin D status. Furthermore, no association found between obesity and related pathologies and the 4 VDR gene polymorphisms.