Vitamin D3: Detailed Characterization of the Substance and Analysis

Abstract

The article examines in detail the issues of standardization of the obtained vitamin D substance using its own original technological scheme, and the possibility of further use of the substance to create a standard sample (SS). The work traces the current problem associated with the lack of production of domestic vitamin D substance. The object of study is the vitamin D substance isolated from natural raw materials. To assess the quality of the obtained substance, quantitative and qualitative determination methods, high-performance liquid chromatography (HPLC) at a wavelength of 265 nm, IR spectroscopy in the emission range of the spectrum from 4000 cm-1 to 400 cm-1, organoleptic method, polarimetry, refractometry and other methods of analysis were used. The obtained vitamin D substance, according to its quality characteristics, can be confidently used not only as a material for the manufacture of SS, but also as a starting substance in the pharmaceutical, food and agricultural sectors of the national economy.